[Research progress in biological activities and oocyte aging-regulating effect of EGCG].

Epigallocatechin gallate (EGCG), the predominant polyphenol in green tea, exerts a spectrum of physiological activities, including antioxidant, anticancer, and anti-inflammatory effects. Emerging research underscores the significance of EGCG in modulating oocyte aging. EGCG can enhance antioxidant defenses, improve mitochondrial functions, and inhibit apoptotic pathways, thereby retarding the aging of oocytes.

Cloning, bioinformatics analysis and expression of the cysteine dioxygenase type 1 (CDO1) gene in domestic yak.

Introduction: The CDO1 gene is an important gene in the taurine synthesis pathway and has been observed to have high expression in ovaries of female mammals. This study aims to explore the conservation of CDO1 gene in domestic yaks, as well as to examine the fundamental characteristics of CDO1 gene and its expression in female yaks.

Methods: Ovarian samples were collected from yaks in the follicular phase, luteal phase and gestation period in this experiment, and their total RNA and protein were extracted.

Characteristics of Nocturnal Boundary Layer over a Subtropical Forest: Implications for the Dispersion and Fate of Atmospheric Species.

The nocturnal boundary layer (NBL) significantly influences the dispersion and fate of atmospheric species at night. Subtropical forests are crucial in carbon and water exchange between the biosphere and the atmosphere. However, the NBL characteristics and their impact on atmospheric species over these forests remain unknown.

Integrated physiological, intestinal microbiota, and metabolomic responses of adult zebrafish (Danio rerio) to subacute exposure to antimony at environmentally relevant concentrations.

The available information regarding the impact of antimony (Sb), a novel environmental pollutant, on the intestinal microbiota and host health is limited. In this study, we conducted physiological characterizations to investigate the response of adult zebrafish to different environmental concentrations (0, 30, 300, and 3000 µg/L) of Sb over a period of 14 days. Biochemical and pathological changes demonstrated that Sb effectively compromised the integrity of the intestinal physical barrier and induced inflammatory responses as well as oxidative stress.

CIRBP Increases the synthesis and secretion of steroid hormones by in yak granulaso cells.

As a regulatory protein that upregulates transcription in response to various stresses, cold-induced RNA-binding protein (CIRBP) is involved in a variety of physiological pathological processes in cells. However, little is known about the role of CIRBP in regulating autophagy and the synthesis and secretion of ovarian steroid hormones (estradiol E2 and progesterone P4). This study aimed to explore whether the synthetic secretion of ovarian steroid hormones is related to CIRBP-regulated autophagy.

During Water Stress, Fertility Modulated by ROS Scavengers Abundant in Arabidopsis Pistils.

Hours after watering plants with 75 mM NaCl, the water potential of reproductive structures precipitously decreases. In flowers with mature gametes, this change in water potential did not alter the rate of fertilization but caused 37% of the fertilized ovules to abort. We hypothesize that the accumulation of reactive oxygen species (ROS) in ovules is an early physiological manifestation associated with seed failure.

LncRNA MEG3 regulates ASK1/JNK axis-mediated apoptosis and autophagy via sponging miR-23a in granulosa cells of yak tertiary follicles.

Apoptosis and autophagy in granulosa cells (GCs) are highly related to follicular development and atresia. It has also been reported that they are related to LncRNA MEG3, miR-23a and apoptosis signal-regulating kinase 1 (ASK-1). However, their relationship to follicular development and the extent to which follicle stimulating hormone (FSH) or luteinizing hormone (LH) can regulate this process remain unknown.

Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro.

After mammalian ovulation, oocytes enter the oviduct, causing oocyte and oviduct changes. Some studies have shown that follicular fluid exosomes (FEVs) play an important role in this regulatory process, but the specific mechanism is remains unclear. Here, we investigate the effect of FEVs on autophagy and on the synthesis and secretion of oviductal glycoprotein 1 (OVGP1) in yak oviduct epithelial cells (OECs).

A multifunctional polymeric additive with a synergistic effect for high-performance lithium-ion batteries.

A new type of tailor-made polymeric additive, poly(ethylene phosphate acrylonitrile), has been proposed as a multifunctional polymeric additive for endowing the carbonate electrolyte with synergistically regulated properties of good flame-retardance, enhanced Li-ion dissociation/transportation behavior and a stable LiFePO cathode electrolyte interphase (CEI) layer. Thus, an ultrahigh stable operation of 1500 cycles at the 20C-rate with a good discharge capacity is obtained.

Modification Effect of Nano-Clay on Mechanical Behavior of Composite Geomaterials: Cement, Nano-Silica and Coastal Soft Soil.

To study the modification effect of nano-clay and nano-SiO on cement-reinforced coastal soft soil, the effects of the nano-SiO and nano-clay on the mechanical properties of cement soil were studied through unconfined compressive and unconsolidated undrained shear tests, and the Duncan-Chang model was used to fit the test results. Results show that adding nano-clay and nano-SiO to cement soil improved its compressive and shear strength. The compressive strength and shear strength increased by 18-57% and 3-32%, respectively, with the increase in nano-clay content in a content range of 0-10%.

Ligand-modified nanocarriers for oral drug delivery: Challenges, rational design, and applications.

Ligand-modified nanocarriers (LMNCs) specific to their targets have attracted increasing interest for enhanced oral drug delivery in recent decades. Although the design of LMNCs for enhanced endocytosis and improved exposure of the loaded drugs through the oral route has received abundant attention, it remains unclear how the design influences their transcellular process, especially the key factors affecting their functions. This review discusses the extracellular and cellular barriers to orally administered LMNCs in the gastrointestinal (GI) tract and new discoveries regarding the GI protein corona and the sequential transport barriers that impede the preplanned movements of LMNCs after oral administration.

Leukemia inhibitory factor enhances the development and subsequent blastocysts quality of yak oocytes .

Leukemia inhibitory factor (LIF) is a multipotent cytokine of the IL-6 family which plays a critical role in the maturation and development of oocytes. This study evaluated the influence of LIF on the maturation and development ability of yak oocytes, and the quality of subsequent blastocysts under culture settings. Different concentrations of LIF (0, 25, 50, and 100 ng/mL) were added during the culture of oocytes to detect the maturation rate of oocytes, levels of mitochondria, reactive oxygen species (ROS), actin, and apoptosis in oocytes, mRNA transcription levels of apoptosis and antioxidant-related genes in oocytes, and total cell number and apoptosis levels in subsequent blastocysts.

Effect of rapamycin treatment on oocyte in vitro maturation and embryonic development after parthenogenesis in yaks.

Autophagy plays an important role in mammalian oocyte maturation and early embryonic development and rapamycin is well known for inducing autophagy. Although previous studies have reported the effects of rapamycin on oocytes in vitro maturation (IVM) in different species, few studies have been reported on the role of rapamycin in yak oocytes IVM and embryonic development. Therefore, the objective of this study was to examine the effect of rapamycin treatment on yak oocytes IVM and early embryonic development.

Transcriptome sequencing reveals differences between leydig cells and sertoli cells of yak.

In this study, we detected the expression of mRNAs, lncRNAs, and miRNAs in primary cultured leydig cells (LCs) and sertoli cells (SCs) of yak by RNA sequencing technology. A total of 84 differently expression mRNAs (DEmRNAs) (LCs vs. SCs: 15 up and 69 down), 172 differently expression lncRNAs (DElncRNAs) (LCs vs.

Integrated analysis of the expression profiles of the lncRNA-miRNA-mRNA ceRNA network in granulosa and cumulus cells from yak ovaries.

Background: Growing oocytes acquire the ability to mature through two-way communication between gametes and surrounding somatic cumulus cells (CCs). Granulosa cells (GCs) support oocyte growth, regulate meiosis progression, and modulate global oocyte transcription activity. However, the proliferation and differentiation of the yak ovary in GCs and CCs remain unclear.

QNE1 is a key flowering regulator determining the length of the vegetative period in soybean cultivars.

The soybean E1 gene is a major regulator that plays an important role in flowering time and maturity. However, it remains unclear how cultivars carrying the dominant E1 allele adapt to the higher latitudinal areas of northern China. We mapped the novel quantitative trait locus QNE1 (QTL near E1) for flowering time to the region proximal to E1 on chromosome 6 in two mapping populations.

Effect of different forms and components of EPS on sludge aggregation during granulation process of aerobic granular sludge.

The characteristics of three forms of extracellular polymeric substances (EPS) and their component proteins (PN) and polysaccharides (PS) during the granulation process of aerobic granular sludge (AGS) and their effects on the aggregation performance of AGS were studied. Meanwhile, Pearson correlation analysis was conducted to explore the correlations between different forms of EPS and their components and sludge properties in the granulation process. The results showed that the AGS reactor could be started within 40 days by using the strategy of decreasing sedimentation time, and the sludge granulation degree was 90.

Effect of EPS and its forms of aerobic granular sludge on sludge aggregation performance during granulation process based on XDLVO theory.

Different forms of extracellular polymeric substances (EPS) play different roles in the formation process of aerobic granular sludge (AGS). This work focused on the contribution of loosely bound EPS (LB-EPS), tightly bound EPS (TB-EPS) and EPS to the aggregation between sludge cells during the start-up of aerobic granular sludge in a sequencing batch reactor. By analyzing the changes of sludge surface characteristics before and after the extraction of each layer of EPS, the contribution of LB-EPS, TB-EPS and EPS to the adhesion and aggregation of sludge cells in the granulation was calculated by surface thermodynamics and the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory.

The nucleolar protein SAHY1 is involved in pre-rRNA processing and normal plant growth.

Although the nucleolus is involved in ribosome biogenesis, the functions of numerous nucleolus-localized proteins remain unclear. In this study, we genetically isolated Arabidopsis thaliana salt hypersensitive mutant 1 (sahy1), which exhibits slow growth, short roots, pointed leaves, and sterility. SAHY1 encodes an uncharacterized protein that is predominantly expressed in root tips, early developing seeds, and mature pollen grains and is mainly restricted to the nucleolus.

Interactions between fish isolates Pseudomonas fluorescens and Staphylococcus aureus in dual-species biofilms and sensitivity to carvacrol.

Biofilm formation is a frequent source of contamination of food products, which results in significant economic losses through microbial spoilage and poses serious health concerns. Little is known about the fate of Staphylococcus aureus in the dual-species biofilms with Pseudomonas fluorescens an important spoiler commonly found in aquatic products. This study evaluates the interactions between mono- or dual-species biofilms formed by P.

Genotyping of Soybean Cultivars With Medium-Density Array Reveals the Population Structure and QTNs Underlying Maturity and Seed Traits.

Soybean was domesticated about 5,000 to 6,000 years ago in China. Although genotyping technologies such as genotyping by sequencing (GBS) and high-density array are available, it is convenient and economical to genotype cultivars or populations using medium-density SNP array in genetic study as well as in molecular breeding. In this study, 235 cultivars, collected from China, Japan, USA, Canada and some other countries, were genotyped using SoySNP8k iSelect BeadChip with 7,189 single nucleotide polymorphisms (SNPs).

Composition and Bioactivity of Essential Oil from Citrus grandis (L.) Osbeck 'Mato Peiyu' Leaf.

'Mato Peiyu' pomelo ( (L.) Osbeck 'Mato Peiyu') leaves from pruning are currently an agricultural waste. The aim of this study was to isolate essential oils from these leaves through steam distillation (SD) and solvent-free microwave extraction (SFME) and to evaluate their applicability to skin care by analyzing their antimicrobial, antioxidant (diphenyl-1-picrylhydrazyl scavenging assay, β-carotene/linoleic acid assay, and nitric oxide scavenging assay), anti-inflammatory (5-lipoxygenase inhibition assay), and antityrosinase activities.

Functional conservation and diversification of the soybean maturity gene E1 and its homologs in legumes.

Gene regulatory networks involved in flowering time and photoperiodic responses in legumes remain unknown. Although the major maturity gene E1 has been successfully deciphered in soybean, knowledge on the functional conservation of this gene is limited to a certain extent to E1 homologs in legumes. The ectopic expression of Phvul.