The Effect of Bovine Trypsin on the Adhesion and pH of Dental Plaque Biofilms: An In Vitro Study.

Objective: The aim of this study was to investigate the effect of bovine trypsin on the adhesion and pH of dental plaque biofilms.

Methods: A multispecies dental plaque biofilm model and a single-species dental plaque biofilm model were established in vitro. Three groups were tested: (1) blank control group (aseptic ultrapure water); (2) negative control group (1M Tris-HCl buffer, pH = 7.

Expert consensus on irrigation and intracanal medication in root canal therapy.

Chemical cleaning and disinfection are crucial steps for eliminating infection in root canal treatment. However, irrigant selection or irrigation procedures are far from clear. The vapor lock effect in the apical region has yet to be solved, impeding irrigation efficacy and resulting in residual infections and compromised treatment outcomes.

The effect of bovine trypsin on dental biofilm dispersion: an in vitro study.

To investigate the degradation effect of bovine trypsin on multispecies biofilm of caries-related bacteria and provide an experimental foundation for the prevention of dental caries. Standard strains of S. mutans, S.

An study on the degradation of multispecies biofilm of periodontitis-related microorganisms by bovine trypsin.

To investigate the degradation effect of bovine trypsin on multispecies biofilm of periodontitis-related bacteria and to provide an experimental reference for exploring new methods for controlling biofilms of periodontitis-related microorganisms, the multispecies biofilm of periodontitis-related microorganisms was established. Standard strains of subsp. , , and were co-cultured to form the biofilm.

[Effect of 17% ethylenediaminetetraacetic acid irrigation on the dentin adhesion of Enterococcus faecalis].

Objective: This work aims to uncover the promoting effect of 17% ethylenediaminetetraacetic acid (EDTA) irrigation on the dentin adhesion of Enterococcus faecalis (E. faecalis).

Methods: Forty-eight half split samples and twelve dentin slices were randomly divided into three experimental groups and one control group.

Roles of Wnt3a and Dkk1 in experimental periodontitis.

Background/purpose: Periodontitis is an inflammatory, destructive disease caused by periodontal bacteria, and its molecular mechanism remains unclear. The aims of this study are to evaluate the expressions of Wnt3a and Dkk1 in experimental periodontitis (EP) and preliminarily explore their roles in periodontal diseases.

Materials And Methods: A total of 64 six-week-old male Sprague-Dawley rats were randomly divided into a normal group and an EP group.

[An animal experiment study on the effect of periodontitis on atherosclerosis].

Objective: To make an animal periodontitis and atherosclerosis compound model, and to study the effects of periodontitis on atherosclerosis.

Methods: 36 Japan rabbits were randomly divided into four groups: Including periodontitis model group, periodontitis and atherosclerosis compound model group, atherosclerosis model group and control group. Periodontitis model was initiated by ligating floss around teeth cervical and oral inoculating with Porphyromonas gingivalis (P.

[Metabonomics study of oral cancer using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry].

Oral cancer is the sixth common cancer in the world, and precisely distinguishing health control, benign and malignant oral tumors is important for the proper and timely selection of therapeutic treatment. In the current study, the metabolic profiles of the plasma, urine and saliva of three groups, consisting of malignant oral tumor patients, benign oral tumor patients and healthy controls, were analyzed using liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Utilizing a partial least squares-discriminant analysis with orthogonal signal correction data filter, the three groups were discriminated based on their plasma, urine and saliva metabolic profiles.

The intervention research on understanding of the AIDS prevention and occupational safety of the dentist in Kunming and west part of Yunnan.

Objective: To investigate the knowledge, attitude of the AIDS prevention and occupational safety in dentist in Kunming and west part of Yunnan and evaluate the effect of education and intervention.

Method: 165 dentists in Yunnan were tested by KABP questionnaire before education. All of the 165 dentists participated a course on AIDS prevention.