Validating Multicenter Cohort Circular RNA Model for Early Screening and Diagnosis of Gestational Diabetes Mellitus.

Background: Gestational diabetes mellitus (GDM) is a metabolic disorder posing significant risks to maternal and infant health, with a lack of effective early screening markers. Therefore, identifying early screening biomarkers for GDM with higher sensitivity and specificity is urgently needed.

Methods: High-throughput sequencing was employed to screen for key circular RNAs (circRNAs), which were then evaluated using reverse transcription quantitative polymerase chain reaction.

Freshwater fungi in the karst plateau wetlands from Guizhou Province, China: taxonomic novelties in Melanommataceae (Pleosporales).

Three isolates of interest were collected during an investigation of freshwater fungi from wetlands in the karst regions of Guizhou Province, Southwest China. Phylogenetic analyses using ITS, LSU, SSU, and gene regions have revealed the placements of these isolates in Melanommataceae (Pleosporales, Dothideomycetes). Based on the morphological and phylogenetic evidence, three new species are introduced: , , and is sister to and forms a basal branch of within Melanommataceae.

Rapid and facile detection of Mycoplasma pneumoniae 16S rRNA based on CHA-FICA system.

Mycoplasma pneumoniae (MP) is a major a rapid, sensitive and reliable method for the detection of 16S ribosomal RNA (16S rRNA) was developed based on catalytic hairpin assembly (CHA) technology and digoxine-biotin double-labeled fluorescence immunochromatography (FICA) strip. The signal amplification achieved by CHA avoids the dependence on expensive thermal cycling equipment. The method can obtain the results within 1 h in the absence of an enzyme, and the minimum detection limit can reach 1 pM.

Tapping line detection and rubber tapping pose estimation for natural rubber trees based on improved YOLOv8 and RGB-D information fusion.

Tapping line detection and rubber tapping pose estimation are challenging tasks in rubber plantation environments for rubber tapping robots. This study proposed a method for tapping line detection and rubber tapping pose estimation based on improved YOLOv8 and RGB-D information fusion. Firstly, YOLOv8n was improved by introducing the CFB module into the backbone, adding an output layer into the neck, fusing the EMA attention mechanism into the neck, and modifying the loss function as NWD to realize multi-object detection and segmentation.

Warming reduces mid-summer flowering plant reproductive success through advancing fruiting phenology in an alpine meadow.

Changes in reproductive phenology induced by warming are happening across the globe, with significant implications for plant sexual reproduction. However, the changes in plant reproductive output (number of flowers and fruits) and success (successful fruits/total flowers) in response to climate change have not been well characterized. Here, we conducted a warming and altered precipitation experiment in an alpine meadow on the eastern Tibetan Plateau to investigate the effects of climate change on the reproductive phenology and success of six common species belonging to two flowering functional groups.

Advancements in the preparation technology of small molecule artificial antigens and their specific antibodies: a comprehensive review.

Small molecules find extensive application in medicine, food safety, and environmental studies, particularly in biomedicine. Immunoassay technology, leveraging the specific recognition between antigens and antibodies, offers a superior alternative to traditional physical and chemical analysis methods. This approach allows for the rapid and accurate detection of small molecular compounds, owing to its high sensitivity, specificity, and swift analytical capabilities.

Phylogeny and taxonomy of two new species in Dictyosporiaceae (Pleosporales, Dothideomycetes) from Guizhou, China.

Two novel species within the family Dictyosporiaceae are described and illustrated from terrestrial habitats on dead culms of bamboo and an unidentified plant, respectively. Through morphological comparisons and the multi-locus phylogenetic analyses of combined LSU, ITS, SSU, and sequence dataset, two species, , are identified. Phylogenetically, both species clustered into a monophyletic clade with strong bootstrap support.

Process optimization and character evaluation of Bletilla striata polysaccharide (BSP) and chitosan (CS) composite hemostatic sponge (BSP-CS).

Bletilla striata polysaccharide (BSP) and chitosan (CS) were chemically cross-linked using oxalyl chloride to prepare a composite hemostatic sponge (BSP-CS), and the process parameters were optimized using the Box-Behnken design (BBD) with response surface methodology. To optimize the performance of the hemostatic sponge, we adjusted the ratio of independent variables, the amount of oxalyl chloride added, and the freeze-dried volume. A series of evaluations were conducted on the hemostatic applicability of BSP-CS.

Three novel species of (Distoseptisporales, Aquapteridosporaceae) from freshwater habitats in Tibetan Plateau, China.

During an investigation of lignicolous freshwater fungi in the Tibetan Plateau, three taxa were collected from freshwater habitats in Xizang, China. The new species possess polyblastic, sympodial, denticles conidiogenous cells and fusiform, septate, with or without sheath conidial, that fit within the generic concept of , and multi-gene phylogeny placed these species within . Detailed morphological observations clearly demarcate three of these from extant species and are hence described as new taxa.

Comprehensive evaluation of serum circHAS2 as a novel diagnostic and prognostic biomarker for gastric cancer.

The diagnosis and screening indicators for gastric cancer (GC) are not satisfactory, resulting in a large number of GC patients being missed and missing the best treatment time. Due to the special structure of circular RNAs (circRNAs), they have a more accurate and powerful ability to detect tumor occurrence. In addition, circHAS2 has been found to promote the proliferation, migration, and invasion of GC cells.

A colloidal gold test strip based on catalytic hairpin assembly for the clinical detection of influenza a virus nucleic acid.

Influenza A epidemics, which occur annually in varying degrees worldwide, is a global challenge to healthcare facilities owing to several limitations of the current detection methods. Therefore, the development of a rapid, convenient, and economical method for the early diagnosis of influenza A will aid clinical treatment and epidemic control. Currently, most of the commonly used clinical rapid tests utilize colloidal gold test strips that detect specific influenza virus antigens but are limited by low sensitivity.

Dual-targeted and dual-sensitive self-assembled protein nanocarrier delivering hVEGI-192 for triple-negative breast cancer.

Breast cancer is a highly prevalent malignancy worldwide among women with an increasing incidence in recent years. Triple-negative breast cancer (TNBC), a specific type of breast cancer, occurs primarily in young women and exhibits large tumor size, high clinical stage, and extremely poor prognosis with a high rate of lymph node, liver, and lung metastases. TNBC is insensitive to endocrine therapy and trastuzumab treatment, and there is an urgent need for effective therapeutics and treatment guidelines.

Thirst in heart failure: A scoping review.

Aim: The aim of this study was to summarise the overall picture of thirst-related research in patients with heart failure.

Design: We conducted a scoping review following the Arskey and O'Malley methodological framework along with the PAGER framework.

Methods: PubMed, CINAHL, Web of Science, Embase, The Cochrane Library, Jonna Briggs Institute, ProQuest Database, Google Scholar, PsycINFO, PQDT, CNKI, Wan Fang, VIP and CBM.

Addition of dNTPs can improve the detection sensitivity of catalytic hairpin assembly.

Ever since the catalytic hairpin assembly (CHA) circuit has been highlighted as a powerful nucleic acid detection tool, nucleic acid detection methods based on CHA have been widely studied. However, the detection sensitivity of CHA-based methods is insufficient. The relatively high background signals resulting from the spontaneous reaction between the two hairpin probes is one of the major reasons for limiting the sensitivity of CHA.

Tapped area detection and new tapping line location for natural rubber trees based on improved mask region convolutional neural network.

Aiming at the problem that the rubber tapping robot finds it difficult to accurately detect the tapped area and locate the new tapping line for natural rubber trees due to the influence of the rubber plantation environment during the rubber tapping operation, this study proposes a method for detecting the tapped area and locating the new tapping line for natural rubber trees based on the improved mask region convolutional neural network (Mask RCNN). First, Mask RCNN was improved by fusing the attention mechanism into the ResNeXt, modifying the anchor box parameters, and adding a tiny fully connected layer branch into the mask branch to realize the detection and rough segmentation of the tapped area. Then, the fine segmentation of the existing tapping line was realized by combining edge detection and logic operation.

A simple and programmable dual-mode aptasensor for the ultrasensitive detection of multidrug-resistant bacteria.

Accurately identifying multidrug-resistant (MDR) bacteria from clinical samples has long been a challenge. Herein, we report a simple and programmable dual-mode aptasensor called DAPT to reliably detect MDR bacteria. The DAPT method comprises two elements, namely the mode of dynamic light scattering (Mode-DLS) for ultrasensitive detection and the mode of fluorescence (Mode-Flu) for reliable quantification as a potent complement.

Occurrence and Morpho-Molecular Identification of Species from Guizhou Province, China.

is an important order of diverse fungal pathogens, saprobes, and endophytes distributed worldwide. Recent studies of in China have discovered a broad range of species, some of which have not been formerly described. In this study, 60 saprobic isolates were obtained from decaying woody hosts in southwestern China.

Additions to the Genus (Apiosporaceae) From Bamboos in China.

has a widespread distribution occurring in various substrates (e.g., air, soil debris, plants, lichens, marine algae and even human tissues).

Unravelling Species Associated with Woody Hosts from Karst Formations (Guizhou) in China.

Though several species have been reported in China, little is known about the species associated with nature reserves in Guizhou province. During a survey of fungi in six nature reserves in Guizhou province of China, thirty-one isolates were collected from different woody hosts. Based on morphology, culture characteristics and molecular phylogenetic analysis, these isolates were characterized and identified.

Ultrasound-guided fine-needle aspiration biopsy of thyroid nodules <10 mm in the maximum diameter: does size matter?

Objective: Ultrasound-guided fine-needle aspiration biopsy (US-FNAB) is a safe and effective method of screening malignant thyroid nodules such as papillary thyroid carcinoma. However, not much data are available regarding the diagnostic efficacy of US-FNAB for papillary thyroid microcarcinoma (≤10 mm in diameter). We aim to compare the diagnostic efficacy of US-FNAB on thyroid nodules between two groups divided by a diameter of 10 mm by correlating the cytological results of US-FNAB with the histopathologic diagnoses in selected patients.

[Comparison of the structures of the medical staff and the operation situations of the departments of critical care medicine between the provincial and county level hospitals of Guizhou Province in 2017].

Objective: To understand the situations of departments of intensive care units (ICUs) of different level hospitals in Guizhou Province, and to provide directions and evidences for improving quality control in critical care medicine.

Methods: A county-level hospital and a provincial-level hospital's comprehensive ICU in Guizhou Province were selected to record and analyze and compare the structural indicators, patient admission and transfer, disease distribution, ventilator associated pneumonia (VAP), intravascular catheter related blood stream infection (CRBSI) and catheter-associated urinary tract infection (CAUTI) of the two hospitals' comprehensive ICU in 2017.

Results: The ICU of the People's Hospital of Suiyang County (county hospital) was found in 2012, and the ICU of the Affiliated Hospital of Guizhou Medical University (provincial hospital) was found in 1994.