End-To-End Automated Intact Protein Mass Spectrometry for High-Throughput Screening and Characterization of Bispecific and Multispecific Antibodies.

Bispecific antibodies (bsAbs) and multispecific antibodies (msAbs) represent a promising frontier in therapeutic antibody development, offering unique capabilities not achievable with traditional monoclonal antibodies. Despite their potential, significant challenges remain due to their increased molecular complexity. One prominent challenge is the correct assembly of light and heavy chains, as improper pairing leads to mispaired or incompletely assembled species that lack therapeutic efficacy and possess undesired properties, impairing the developability, manufacturability, and safety.

Composition and Bioactivity of Chlorogenic Acids in Vegetable and Conventional Sweet Potato Vine Tips.

Sweet potato vine tips are abundant in chlorogenic acid (CGA). In this study, CGA was extracted from vegetable and conventional sweet potato vine tips using ethanol, followed by subsequent purification of the extract through a series of sequential steps. Over 4 g of the purified product was obtained from 100 g of sweet potato vine tip powder, producing more than 85% of purified CGA.

Observation of Heavy-Chain C-Terminal Des-GK Truncation in Recombinant and Human Endogenous IgG4.

Therapeutic IgG mAbs have shown presence of three variations of their heavy chain C-termini, including the unprocessed C-terminal lysine, the processed C-terminal lysine, and C-terminal amidation. These variants are also present in endogenous human IgGs, although the level of unprocessed C-terminal lysine is very low. Here we report a new heavy-chain C-terminal variant, i.

Vertically aligned NiS/CoS/MoS nanosheet array as an efficient and low-cost electrocatalyst for hydrogen evolution reaction in alkaline media.

Recently, the rational design of non-precious metal electrocatalysts for highly efficient hydrogen evolution reaction (HER) in alkaline media has received considerable interests in sustainable and renewable energy researches. Herein, vertically aligned and interconnected NiS/CoS/MoS nanosheet arrays on Ni foam were prepared by a two-step procedure that conducted by the hydrothermal synthesis of Ni-Co molybdate nanosheet array as the precursor and followed by the vapor phase sulfurization to achieve in situ conversion. Basing on the scanning electron microscopy (SEM) and transmission electron microscopy (TEM) characterizations, it can be found that the honeycomb-like structure of the Ni-Co molybdate nanosheet array was well preserved after the sulfurization process.

A flower-like CoS/MoS heteronanosheet array as an active and stable electrocatalyst toward the hydrogen evolution reaction in alkaline media.

CoS/MoS heteronanosheet arrays (HNSAs) with vertically aligned flower-like architectures are fabricated through topotactic sulfurization of CoMoO nanosheet array (NSA) precursors on conductive Ni foam. CoMoO NSAs are prepared by a self-template hydrothermal method without using any hard template and surfactant. Benefiting from a 3D flower-like architecture constituted by ultrathin nanosheets with abundant exposed heterointerfaces as highly active sites and predesigned void spaces, the as-synthesized CoS/MoS HNSAs exhibit an excellent hydrogen evolution reaction (HER) performance with a low overpotential of 50 mV at 10 mA cm, and a small Tafel slope of 76 mV dec in 1.

Nitrogen-Doped Porous Carbon Nanosheets Strongly Coupled with MoC Nanoparticles for Efficient Electrocatalytic Hydrogen Evolution.

Exploring earth-abundant and noble metal-free catalysts for water electrolysis is pivotal in renewable hydrogen production. Herein, a highly active electrocatalyst of nitrogen-doped porous carbon nanosheets coupled with MoC nanoparticles (MoC/NPC) was synthesized by a novel method with high BET surface area of 1380 m g using KOH to activate carbon composite materials. The KOH plays a key role in etching out MoS to produce Mo precursor; simultaneously, it corrodes carbon to form porous structure and produce reducing gas such as H and CO.

Design of Bionic Cochlear Basilar Membrane Acoustic Sensor for Frequency Selectivity Based on Film Triboelectric Nanogenerator.

Sensorineural hearing loss tops the list of most suffering disease for the sake of its chronic, spirit pressing, and handicapped features, which can happen to all age groups, from newborns to old folks. Laggard technical design as well as external power dependence of conventional cochlear implant cumbers agonized patients and restrict its wider practical application, driving researchers to seek for fundamental improvement. In this paper, we successfully proposed a novel bionic cochlear basilar membrane acoustic sensor in conjugation with triboelectric nanogenerator.