Methane mitigation via the nitrite-DAMO process induced by nitrate dosing in sewers.

Nitrate or nitrite-dependent anaerobic methane oxidation (n-DAMO) is a microbial process that links carbon and nitrogen cycles as a methane sink in many natural environments. This study demonstrates, for the first time, that the nitrite-dependent anaerobic methane oxidation (nitrite-DAMO) process can be stimulated in sewer systems under continuous nitrate dosing for sulfide control. In a laboratory sewer system, continuous nitrate dosing not only achieved complete sulfide removal, but also significantly decreased dissolved methane concentration by ∼50 %.

Enhanced mechanistic insights and performance optimization: Controlling methane and sulfide in sewers using nitrate dosing strategies.

Nitrate has been used for nearly 80 years as a chemical to control problematic gases in the sewer system. However, few studies have explored simultaneous control in biofilm and sediment using different strategies. Here, we introduced a nitrate dosing method involving an initial high shock followed by low level dosing, tested at two distinct frequencies in a lab-scale sewer reactor <110 days.

In-situ advanced oxidation of sediment iron for sulfide control in sewers.

Sulfide control is a significant problem in urban sewer management. Although in-sewer dosing of chemicals has been widely applied, it is prone to high chemical consumption and cost. A new approach is proposed in this study for effective sulfide control in sewers.

The advantage of a two-stage nitrification method for fertilizer recovery from human urine.

Recycling nutrients (nitrogen, phosphorus, and potassium) from human urine can potentially offset more than 13% of global agricultural fertilizer demand. Biological nitrification is a promising method for converting volatile ammonia in high-strength human urine into stable ammonium nitrate (a typical fertilizer), but it is usually terminated in the intermediate production of nitrite due to the inhibition of nitrite-oxidizing bacteria by free nitrous acid (FNA). This study aimed to develop a stable nitrification process in a unique two-stage bioreactor by removing critical barriers associated with FNA inhibition.

Reducing sulfide and methane production in gravity sewer sediments through urine separation, collection and intermittent dosing.

Sulfide and methane production are a major concern in sewer management. Many solutions with the use of chemicals have been proposed yet incurring huge costs. Here, this study reports an alternative solution to reduce sulfide and methane production in sewer sediments.

Changes in the Mitochondria-Related Nuclear Gene Expression Profile during Human Oocyte Maturation by the IVM Technique.

To address which mitochondria-related nuclear differentially expressed genes (DEGs) and related pathways are altered during human oocyte maturation, single-cell analysis was performed in three oocyte states: in vivo matured (M-IVO), in vitro matured (M-IVT), and failed to mature in vitro (IM-IVT). There were 691 DEGs and 16 mitochondria-related DEGs in the comparison of M-IVT vs. IM-IVT oocytes, and 2281 DEGs and 160 mitochondria-related DEGs in the comparison of M-IVT vs.

Simultaneous control of sulfide and methane in sewers achieved by a physical approach targeting dominant active zone in sediments.

Sewer sediments not only induce sewer blockages, but also contributes to significant sulfide and methane productions in gravity sewer systems. Chemical control of sulfide and methane production is extremely expensive. This study aims to propose a novel physical control approach-intermittent surface sediment flushing to synchronously address sediment-induced multiple issues.

The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro.

Background: Lysophosphatidic acid-supplemented culture medium significantly increases the oocyte maturation rate in vitro. However, potential targets and pathways involved remain unknown.

Methods: A total of 43 women, who underwent cesarean section and aged between 18 and 35 years with good health, were included in this study.

Single-cell multiomic analysis of in vivo and in vitro matured human oocytes.

Study Question: Are there any differences between in vivo (IVV) and in vitro (IVT) matured metaphase II (MII) oocytes at the molecular level?

Summary Answer: Between IVV and IVT oocytes, 507 differentially expressed genes (DEGs) were identified; the non-CpG methylomes were significantly different, but the CpG methylomes and genomic copy number variations (CNVs) were similar.

What Is Known Already: A previous study using microarray and single-cell RNA-seq analysis revealed that numerous genes were differentially expressed between IVV and IVT oocytes. Independent studies of DNA methylation profiling in human oocytes have revealed negative correlations between gene transcription and the DNA methylation level at gene promoter regions.

Exploring the reciprocal relations between mothers' and fathers' use and attitudes of corporal punishment in China: A cross-lagged analysis.

Background: Corporal punishment is a commonly used form of disciplinary technique. Sanctified parental attitudes of corporal punishment have been found to be a significant predictor of parental use of corporal punishment in previous research, while little is currently known about the reciprocal relations between parental use and their attitudes of corporal punishment.

Objective: This research aimed to examine the reciprocal relations between mothers' and fathers' use and attitudes of corporal punishment in China.