Characterization of Klebsiella pneumoniae Carbapenemase (KPC)-14, a KPC Variant Conferring Resistance to Ceftazidime-Avibactam in the Extensively Drug-resistant ST463 Pseudomonas aeruginosa Clinical Isolate.

Objectives: We studied two Klebsiella pneumoniae carbapenemase (KPC)-14 variants from clinical Pseudomonas aeruginosa isolates (C137 and C159) to better understand the genomic diversity, mechanisms, and genes that confer antibiotic resistance and pathogenicity.

Methods: Genomic DNA from C137/159 was subjected to Illumina and Oxford Nanopore sequencing. Horizontal transmission of the plasmid was evaluated using cloning experiments.

Viral load dynamics and disease severity in patients infected with SARS-CoV-2 in Zhejiang province, China, January-March 2020: retrospective cohort study.

Objective: To evaluate viral loads at different stages of disease progression in patients infected with the 2019 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during the first four months of the epidemic in Zhejiang province, China.

Design: Retrospective cohort study.

Setting: A designated hospital for patients with covid-19 in Zhejiang province, China.

Tunable shape transformation of freezing liquid water marbles.

Liquid water marbles coated with fumed silica nanoparticles exhibit various shape transformations upon freezing which are dependent on the hydrophobicity of the nanoparticles. The shape can be recovered during re-melting. For marbles coated with the most hydrophobic particles, a vertically prolonged morphology with a pointed protrusion on the top is formed on freezing.