In vitro reconstitution of meiotic DNA double-strand-break formation.

The Spo11 complex catalyses the formation of DNA double-strand breaks (DSBs), initiating meiotic recombination-a process that is essential for fertility and genetic diversity. Although the function of Spo11 has been known for 27 years, previous efforts to reconstitute DSB formation in vitro have been unsuccessful. Here we biochemically characterize the mouse SPO11-TOP6BL protein complex, and show that this complex cleaves DNA and covalently attaches to the 5' terminus of DNA breaks in vitro.

DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells.

Ribosome biogenesis plays a pivotal role in maintaining stem cell homeostasis, yet the precise regulatory mechanisms governing this process in mouse embryonic stem cells (mESCs) remain largely unknown. In this investigation, we ascertain that DEAD-box RNA helicase 10 (DDX10) is indispensable for upholding cellular homeostasis and the viability of mESCs. Positioned predominantly at the nucleolar dense fibrillar component (DFC) and granular component (GC), DDX10 predominantly binds to 45S ribosomal RNA (rRNA) and orchestrates ribosome biogenesis.

Developing a city-wide, community-engaged cancer disparities research agenda.

Introduction: In response to high levels of cancer disparities in Philadelphia, PA, three NCI-designated clinical cancer centers formed Philadelphia Communities Conquering Cancer (PC3) to bring stakeholders together and establish infrastructure for future cancer reducing initiatives. The PC3 coalition aimed to develop a prioritized cancer disparities research agenda in order to align cancer center resources and research interests with the concerns of the community about cancer, and to ensure that initiatives were patient- and community-centered.

Methods: Agenda development activities culminated in a city-wide cancer disparities conference.

TEAD2 initiates ground-state pluripotency by mediating chromatin looping.

The transition of mouse embryonic stem cells (ESCs) between serum/LIF and 2i(MEK and GSK3 kinase inhibitor)/LIF culture conditions serves as a valuable model for exploring the mechanisms underlying ground and confused pluripotent states. Regulatory networks comprising core and ancillary pluripotency factors drive the gene expression programs defining stable naïve pluripotency. In our study, we systematically screened factors essential for ESC pluripotency, identifying TEAD2 as an ancillary factor maintaining ground-state pluripotency in 2i/LIF ESCs and facilitating the transition from serum/LIF to 2i/LIF ESCs.

Functional dissection of PRC1 subunits RYBP and YAF2 during neural differentiation of embryonic stem cells.

Polycomb repressive complex 1 (PRC1) comprises two different complexes: CBX-containing canonical PRC1 (cPRC1) and RYBP/YAF2-containing variant PRC1 (vPRC1). RYBP-vPRC1 or YAF2-vPRC1 catalyzes H2AK119ub through a positive-feedback model; however, whether RYBP and YAF2 have different regulatory functions is still unclear. Here, we show that the expression of RYBP and YAF2 decreases and increases, respectively, during neural differentiation of embryonic stem cells (ESCs).

DNA Damage Atlas: an atlas of DNA damage and repair.

DNA damage and its improper repair are the major source of genomic alterations responsible for many human diseases, particularly cancer. To aid researchers in understanding the underlying mechanisms of genome instability, a number of genome-wide profiling approaches have been developed to monitor DNA damage and repair events. The rapid accumulation of published datasets underscores the critical necessity of a comprehensive database to curate sequencing data on DNA damage and repair intermediates.

Community Outreach and Engagement at U.S. Cancer Centers: Notes from the Third Cancer Center Community Impact Forum.

Community outreach and engagement (COE) is a fundamental activity of cancer centers as they aim to reduce cancer disparities in their geographic catchment areas. As part of COE, NCI-Designated Cancer Centers must monitor the burden of cancer in their catchment area, implement and evaluate evidence-based strategies, stimulate catchment area relevant research, support clinical trial enrollment, and participate in policy and advocacy initiatives, in addition to other responsibilities. The Cancer Center Community Impact Forum (CCCIF) is a national annual meeting of COE professionals who work at or with cancer centers across the country.

Effects of blood flow restriction training on bone turnover markers, microstructure, and biomechanics in rats.

Objective: The present study aimed to investigate the effects of blood flow restriction training on muscle strength, bone tissue structure material, and biomechanical properties in rats applying various exercise interventions and to analyze the process by identifying the bone turnover markers, it provides a theoretical basis for the application of BFRT in clinical rehabilitation.

Methods: A total of 24, 3-month-old male SD (Sprague Dawley) rats were randomly divided into pressurized control group (CON, n=6), low-intensity training group (LIRT, n=6), high-intensity training group (HIRT, n=6), and blood flow restriction training group (LIBFR, n=6) for 8-week ladder-climbing exercises. The pressured control group were given only ischemia treatments and did not undertake any burden.

Heart Rate Estimation from Incomplete Electrocardiography Signals.

As one of the most remarkable indicators of physiological health, heart rate (HR) has become an unfailing investigation for researchers. Unlike many existing methods, this article proposes an approach to implement short-time HR estimation from electrocardiography in time series missing patterns. Benefiting from the rapid development of deep learning, we adopted a bidirectional long short-term memory model (Bi-LSTM) and temporal convolution network (TCN) to recover complete heartbeat signals from those with durations are less than one cardiac cycle, and the estimated HR from recovered segment combining the input and the predicted output.

CTCF functions as an insulator for somatic genes and a chromatin remodeler for pluripotency genes during reprogramming.

CTCF mediates chromatin insulation and long-distance enhancer-promoter (EP) interactions; however, little is known about how these regulatory functions are partitioned among target genes in key biological processes. Here, we show that Ctcf expression is progressively increased during induced pluripotency. In this process, CTCF first functions as a chromatin insulator responsible for direct silencing of the somatic gene expression program and, interestingly, elevated Ctcf expression next ensures chromatin accessibility and contributes to increased EP interactions for a fraction of pluripotency-associated genes.

Controlling the Spreading of Nanoliter-Scale Droplets on the Fibers of Fabrics for Enhancing Image Quality and Ink Utilization.

Electronic and traditional textiles have been widely manufactured through inkjet printing. However, nanoliter-scale ink droplets tend to excessively spread along the fiber direction, which results in poor image quality and low ink utilization. Here, hydroxyethyl cellulose (HEC) and hydroxypropyl methyl cellulose (HPMC) were introduced to control the spreading of nanoliter-scale droplets on cotton fabrics.

Effects of standing and walking on plantar pressure distribution in recreational runners before and after long-distance running.

With marathon-running grew in popularity, the effect of long-distance running on plantar pressure has been more attractive. It has been proposed that long-distance running influences the deviation in the center of pressure (COP) during standing and the changes to plantar pressure during walking. The objective of this study was to observe the effects on the COP motion amplitude of static standing and the plantar pressure distribution of walking after long-distance running.

Hierarchical BiMoO microsphere photocatalysts modified with polypyrrole conjugated polymer for efficient decontamination of organic pollutants.

To effectively degrade organic pollutants in wastewater, visible-light-driven BiMoO/PPy hierarchical heterogeneous photocatalysts were prepared through a solvothermal method and the following in-situ chemical oxidation polymerization. Compared with pristine BiMoO photocatalyst, the composite photocatalysts exhibited dramatically improved photocatalytic activity and photostability towards the degradation of methylene blue dye and tetracycline antibiotic. BiMoO/PPy-80 sample achieved the highest photocatalytic degradation rates for methylene blue dye (93.

Green and Efficient Inkjet Printing of Cotton Fabrics Using Reactive Dye@Copolymer Nanospheres.

Digital inkjet printing of textiles possesses great advantages like high efficiency and flexible production, but the challenges like the risk of causing serious environmental problems due to the large usage of dyes and chemicals still remain a matter of concern. In response to this problem, herein, a novel kind of reactive dye@copolymer nanosphere was prepared through the adsorption of C. I.

Effects of Viscosity and Surface Tension of a Reactive Dye Ink on Droplet Formation.

In textile inkjet printing, understanding the effect of viscosity and surface tension of a reactive dye ink on droplet formation is of great significance. As an organic ecofriendly solvent, polyethylene glycol with a molecular weight of -400 g/mol (PEG400) was used to prepare reactive dye inks with or without Surfynol 465 (S465) to explain separately how viscosity and surface tension affect the droplet formation of a reactive dye ink. The intermolecular interactions in the ink and physical properties of the ink were investigated by measuring the visible absorption spectra, hydrodynamic radius, viscosity, and surface tension.

R-loops coordinate with SOX2 in regulating reprogramming to pluripotency.

R-loops modulate genome stability and regulate gene expression, but the functions and the regulatory mechanisms of R-loops in stem cell biology are still unclear. Here, we profiled R-loops during somatic cell reprogramming and found that dynamic changes in R-loops are essential for reprogramming and occurred before changes in gene expression. Disrupting the homeostasis of R-loops by depleting RNaseH1 or catalytic inactivation of RNaseH1 at D209 (RNaseH1) blocks reprogramming.

Jetting Performance of Polyethylene Glycol and Reactive Dye Solutions.

The jetting performance of dye inks determines the image quality, production efficiency, and lifetime of the print head. In the present study, we explored the jetting performance of mixed solutions of polyethylene glycol (PEG) and reactive dye by testing the visible absorption spectra, rheological properties, and surface tension, in addition to the observation of droplet formation. The results indicate that PEG macromolecules could change the aggregate groups of Red 218 molecules into smaller ones through hydrophobic interactions and separation effect.

Inkjet Printable and Self-Curable Disperse Dyes/P(St-BA-MAA) Nanosphere Inks for Both Hydrophilic and Hydrophobic Fabrics.

Low-water-soluble disperse dyes possess a broad color gamut and good durability, but they need chemical or physical modification before being used in inks and can only be applied to several kinds of hydrophobic fabrics. In this work, disperse dyes/P(St-BA-MAA) nanospheres (known as DPN) absorbed by sodium nitrilotriacetate (known as NTA@DPN) were prepared and applied into ink formulations, which exhibited high dye fixation, long-term stability and self-curable ability without addition of any binder. Transmission electron microscopy (TEM) images showed the nanospheres have homogeneous core-shell spherical shape and the average diameter increased by 20.

Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs.

Induced pluripotent stem cells can be derived from somatic cells through ectopic expression of transcription factors or chemical cocktails. Chemical iPSCs (C-iPSCs) and OSKM-iPSCs (4F-iPSCs) have been suggested to have similar characteristics to mouse embryonic stem cells (mESCs). However, their epigenetic equivalence remains incompletely understood throughout the genome.

Effects of long-term high-load exercise on the anatomy of the hip joints: a preliminary report.

The aim of this study was to assess the impact of different sports on hip joint anatomic parameters in athletes. This was a cross-sectional study of eight male trampolinists, eight male weightlifters, and eight male volunteers enrolled in July 2014. Hip joint anatomic parameters were measured from plain radiographs taken at the level of the lesser trochanter center.

RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions.

RNA-binding proteins (RBPs), in addition to their functions in cellular homeostasis, play important roles in lineage specification and maintaining cellular identity. Despite their diverse and essential functions, which touch on nearly all aspects of RNA metabolism, the roles of RBPs in somatic cell reprogramming are poorly understood. Here we show that the DEAD-box RBP DDX5 inhibits reprogramming by repressing the expression and function of the non-canonical polycomb complex 1 (PRC1) subunit RYBP.

A Comparison of a Multi-body Model and 3D Kinematics and EMG ofDouble-leg Circle on Pommel Horse.

The purpose of this study was to establish a multi-segment dynamic model in the LifeMOD to examine kinematics of the center of mass and foot, and muscle forces of selected upper extremity muslces during a double-leg circle (DLC) movement on pommel horse in gymnastics and compared with three-dimensional kinematics of the movement and surface electromyographic (sEMG) activity of the muscles. The DLC movement of one elite male gymnast was collected. The three-dimensional (3D) data was imported in the Lifemod to create a full-body human model.