High-speed atomic force microscope combined with single-molecule fluorescence microscope.

High-speed atomic force microscopy (HS-AFM) and total internal reflection fluorescence microscopy (TIRFM) have mutually complementary capabilities. Here, we report techniques to combine these microscopy systems so that both microscopy capabilities can be simultaneously used in the full extent. To combine the two systems, we have developed a tip-scan type HS-AFM instrument equipped with a device by which the laser beam from the optical lever detector can track the cantilever motion in the X- and Y-directions.

Organization of color-selective neurons in macaque visual area V4.

Cortical area V4 in monkeys contains neurons that respond selectively to particular colors. It has been controversial how these color-selective neurons are spatially organized in V4. One view asserts that color-selective neurons are organized in columns with different colors orderly mapped across the cortex, whereas other studies have found no evidence for columnar organization or any other clustered structure.