Publications by authors named "Yasushi Konomi"
Article Synopsis
- - This study assessed the effectiveness of the ID NOW Influenza A & B 2 test, which provides rapid molecular testing for influenza within 13 minutes, comparing it to existing testing methods.
- - The analysis included 254 nasopharyngeal swabs and 271 aspirates from both children and adults, with ID NOW 2 showing excellent sensitivity (up to 100%) and specificity (100%) for detecting Influenza A and B.
- - Overall, ID NOW 2 proved to be a reliable diagnostic tool for influenza, maintaining performance regardless of the timing of illness onset or patient age, making it beneficial for clinical use and infection control.
Suspension Madin-Darby canine kidney (MDCK) cells (MDCK-N), adherent MDCK cells (MDCK-C), and adherent rhesus monkey kidney LLC-MK2 cells (LLC-MK2D) were systematically evaluated for the preparation of influenza vaccine seed viruses for humans on the basis of primary virus isolation efficiency, growth ability, genetic stability of the hemagglutinin (HA) and neuraminidase (NA) genes, and antigenic properties in hemagglutination inhibition (HI) test of each virus isolate upon further passages. All the subtypes/lineages of influenza viruses (A(H1N1), A(H1N1)pdm09, A(H3N2), B-Victoria, and B-Yamagata) were successfully isolated from clinical specimens by using MDCK-N and MDCK-C, whereas LLC-MK2D did not support virus replication well. Serial passages of A(H1N1) viruses in MDCK-N and MDCK-C induced genetic mutations of HA that resulted in moderate antigenic changes in the HI test.
View Article and Find Full Text PDFWe evaluated Clearline Influenza A/B/(H1N1)2009, a new multi-line immunochromatographic assay for rapid detection of antigens of influenza A (Flu A), B (Flu B), and A(H1N1)2009 viruses. Clearline detected Flu A, Flu B, and A(H1N1)2009 viruses with a detection limit of 4.6 × 10(3) to 7.
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