Effect of sandblasting and/or priming treatment on the shear bond strength of self-adhesive resin cement to CAD/CAM blocks.

The aim of this study was to investigate the effect of two different priming agents and/or sandblasting on the shear bond strength of self-adhesive resin cement to the resin composite for core build-up to CAD/CAM blocks. A CAD/CAM ceramic block (GN I CERAMIC BLOCK, GC) and a CAD/CAM resin composite block (CERASMART 270, GC), a self-adhesive resin cement (G-CEM ONE, GC) and two different primers, i.e.

Effect of surface treatment of CAD/CAM resin composites on the shear bond strength of self-adhesive resin cement.

This study examined the effects of sandblasting, hydrofluoric acid etching and priming on the shear bond strength of self-adhesive resin cement between seven different CAD/CAM resin composites and a resin composite core material at 24-h after cement mixing. Five surface treatments [control (C), sandblasting (S), priming (P), sandblasting with priming (SP), and 9% HF etching with priming (HFP)] were performed respectively for disc specimens of CAD/CAM blocks. There were no significant differences in bond strength among the C, S, and P, except for one block (p>0.

Technique for simple apatite coating on a dental resin composite with light-curing through a micro-rough apatite layer.

Tooth root surfaces restored with dental resin composites exhibit inferior biocompatibility. The objective of this study was to develop a simple technique for coating apatite onto a resin composite to improve its surface biocompatibility. First, we fabricated a polymer film coated with a micro-rough apatite layer and pressed it (coating-side down) onto a viscous resin composite precursor.

Biological Properties of the Aggregated Form of Chitosan Magnetic Nanoparticle.

Background/aim: Chitosan-coated iron oxide nanoparticles (Chi-NP) have gained attention because of their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. In this study, we investigated various biological properties of Chi-NP.

Materials And Methods: Chi-NP was prepared by mixing magnetic NP with chitosan FL-80.

Effect of storage conditions on mechanical properties of resin composite blanks for CAD/CAM crowns.

The objective of this study was to examine the effects of storage conditions on the flexural strengths of resin composite materials for CAD/CAM restorations. Seven commercially available resin composite CAD/CAM blanks were examined. Rectangular specimens (4.

Characteristics of a new dental stone mixed by shaking.

This study evaluated the physical and mechanical properties of a dental stone mixed by shaking. A shake-mix dental stone (Shake! Mix STONE; SM) was characterized in comparison with three conventional dental stones. The fluidity at pouring time, setting time, density, powder particle distributions, linear setting expansion, compressive strength and surface reproduction of detail for dental stones were investigated.

Effects of surface microtopography of titanium disks on cell proliferation and differentiation of osteoblast-like cells isolated from rat calvariae.

The surface topography of implant fixture is an important factor affecting the osseointegration. We herein demonstrated the effects of surface microtopography of titanium disks on proliferation and differentiation of osteoblast-like cells isolated from rat calvariae. Titanium disks with machine surface (MS), rough surface (R1) and rough surface combined with small cavities (R2) were used in an in vitro culture system.

Influence of monomer content on the viscoelasticity, water sorption and solubility of experimental fluorinated soft lining materials.

The influence of monomer content on the viscoelasticity, water sorption and solubility of experimental fluorinated soft lining materials was investigated. Changes in the viscoelastic properties of the materials were also examined after thermal cycling. Four fluorinated soft lining materials containing different amounts of methoxy diethylene glycol methacrylate (MDGMA) and tridecafluorooctyl methacrylate (13FMA) were prepared.

Retention of crowns cemented on implant abutments with temporary cements.

This study was to examine the retentive force of crowns to implant abutments with commercial temporary cements. Six different temporary cements were investigated. Cast crowns were cemented to the abutments using each cement and their retentive forces to abutments were determined 7 or 28 days after cementing (n=10).

Effect of composition of experimental fluorinated soft lining materials on bond strength to denture base resin.

The purpose of the present study was to investigate the effect of the composition of experimental fluorinated soft lining materials on bond strength to denture base resin. Vinylidene fluoride/hexafluoro propylene copolymer (2-6F), tridecafluorooctyl methacrylate (13FMA), methoxy diethylene glycol methacrylate (MDGMA), and silica (as filler) were used for fabrication of the experimental soft lining materials. Nine experimental soft lining materials having various compositions of 2-6F, 13FMA, and MDGMA were prepared.

Effects of TiO2 nanoparticles on cytotoxic action of chemotherapeutic drugs against a human oral squamous cell carcinoma cell line.

Background: Despite the rapid development of nanotechnology, the biological significance of TiO2 nanoparticles (NPs), possibly released from dental materials, is not well-understood. We investigated the effect of TiO2 NPs on the sensitivity of human oral squamous cell carcinoma (OSCC) cell line (HSC-2) to five popular chemotherapeutic agents.

Materials And Methods: Viable cell number was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method.

Protection against copper-induced cytotoxicity by inclusion of gold.

Background: We previously reported that contact with copper (Cu) induced immediate cell death via an oxidation-involved mechanism, and the Cu-induced oxidation and cell death were effectively alleviated under hypoxic conditions. In order to explore alternative strategies for the protection from the Cu-induced cytotoxicity, we investigated whether the inclusion of gold (Au) in the Cu plate, as alloy,has a protective effect.

Materials And Methods: Human gingival fibroblast (HGF) cells, established from periodontal tissues, were inoculated on Au/Cu alloy of different Au ratios.

Quantification of enhanced osteoblastic adhesion to ultraviolet-treated titanium plate.

Although the advantage of ultraviolet (UV) irradiation of titanium plates for the attachment of osteoblast is known, the details of the experimental conditions have not been described in previous literature. We established optimal conditions of UV irradiation of titanium plate for the adhesion of mouse osteoblast MC3T3-E1 cells. The viable cell number was determined by MTT method.

Type of cell death induced by seven metals in cultured mouse osteoblastic cells.

The use of dental metal alloys in the daily clinic makes it necessary to evaluate the cytotoxicity of eluted metal components against oral cells. However, the cytotoxic mechanism and the type of cell death induced by dental metals in osteoblasts have not been well characterized. This study investigated the cytotoxicity of seven metals against the mouse osteoblastic cell line MC3T3-E1.

Effect of contact with titanium alloys on the proliferation of mouse osteoblastic cells in culture.

This study was aimed at studying the effect of contact with titanium alloy plates of different surface textures on the proliferative capability of mouse osteoblastic MC3T3-E1 cells. First, the proliferation characteristics of MC3T3-E1 cells were investigated. MC3T3-E1 cells showed a high capacity for proliferation and survived for a long period even under nutritionally starved conditions.

Effect of water on shear strength of glass ionomer cements for luting.

This study examined the effect of ambient water on the shear strength of glass ionomer cements for luting. Disk specimens were fabricated from four commercially available glass ionomer cements with different setting modes. At one hour after the start of mixing, the specimens were stored at 37 degrees C for 24 and 168 hours in dry condition or in deionized water.

Disruption of amino acid metabolism in human myelogenous leukemic cell lines destined to die after contact with metal plates.

Changes in amino acid metabolism during cell death of human myelogenous leukemic cell lines (HL-1, ML-1, KG-1) induced by contact with gold (Au), silver (Ag) or palladium (Pd) were investigated. All three leukemic cell lines consumed glutamine and serine at the highest rate (amounting to 50%-58% and 12%-16% of the total amino acid consumption, respectively). HL-60 cell growth was slightly stimulated by contact with any metal plate.

Rapid changes in amino acid and polyamine metabolism during copper-induced cell death of human gingival fibroblast.

There are very few studies on the interaction between dental alloys and oral tissues. The effect of direct contact with copper (Cu) on the cellular function of human gingival fibroblast (HGF) derived from the periodontal tissues was investigated. When HGF cells were inoculated onto a Cu plate, the viability of HGF cells immediately declined.

Effect of saliva, epigallocatechin gallate and hypoxia on Cu-induced oxidation and cytotoxicity.

We have previously reported that contact with copper (Cu) induced immediate cell death via an oxidation-involved mechanism in human promyelocytic leukemic HL-60 cells, whereas contact with other metals (Au, Ag, Pd) produced no discernible effect. In the present study, we investigated the conditions under which Cu-induced oxidative stress can be reduced. Contact with a Cu plate in the absence of cells enhanced the rate of consumption of cystine to the greatest extent, followed by that of methionine and histidine.

Biological impact of contact with metals on cells.

In order to investigate the in vivo effect of metals used in dentistry, we investigated the effect of direct contact with metal plates (20 x 20 x 0.5 mm3) made of gold (Au), silver (Ag), copper (Cu) or palladium (Pd) on human promyelocytic leukemic HL-60 cells grown in RPMI1640 medium supplemented with 10% fetal bovine serum. When 0.

Correlation between the strength of glass ionomer cements and their bond strength to bovine teeth.

This study examined the possible correlation between the strength of glass ionomers and their adhesive strength to bovine teeth. The shear bond strengths of three different brands of glass ionomer mixed at four different P/L ratios to bovine teeth were measured 24 hours after the cement specimens were prepared. The correlation between shear bond strength and mechanical strength reported in our previous study was also examined.

Relationship between the strength of glass ionomers and their adhesive strength to metals.

Objectives: The objective of this study was to examine the possible correlation between the strength of glass ionomers and their adhesive strength to metals.

Methods: Three different brands of glass ionomers mixed at four different P/L ratios were evaluated. The compressive and diametral tensile strengths of the cements were determined.