Publications by authors named "Yasuhito Kosugi"
Super-resolution confocal live imaging microscopy (SCLIM) we developed provides high-speed, high-resolution, three- and four-dimensional, and multicolor simultaneous imaging. Using this technology, we are now able to observe the fine details of various dynamic events going on in living cells, such as membrane traffic and organelle dynamics. The retention using selective hooks (RUSH) system is a powerful tool to control synchronous release of natural cargo proteins of interest from the endoplasmic reticulum in mammalian cells.
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- Recent advancements in life sciences emphasize the need for real-time observation of biological processes in their natural environments, yet current 3D imaging techniques for thick specimens are limited in speed and resolution.
- The study improves spinning disk confocal microscopy by increasing interpinhole distance to reduce pinhole cross-talk and using two-photon excitation to prevent out-of-focus light, enhancing focal discrimination.
- These modifications allow for significantly better visualization of cellular structures in live model organisms, showcasing higher-resolution imaging capabilities.
We have developed a high-speed confocal laser microscope. A microlens-array disk set in front of a pinhole-array disk improved optical efficiency more than ten times compared with that of conventional Nipkow confocal microscopy. This new microscope achieves a high-speed measurement of 1 frame/ms.
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