Publications by authors named "Yasuhito Chida"
Article Synopsis
- Cryopreservation is the long-term storage of mammalian cells, typically using serum, which can introduce contamination and quality control issues.
- This study explores rakkyo fructan, derived from Japanese shallots, as a serum-free alternative cryoprotectant, showing its effectiveness in preserving mouse hybridoma cells.
- The combination of rakkyo fructan with DMSO improved protection against freezing stress, allowing mammalian cell lines to maintain functionality post-thawing, positioning rakkyo fructan as a promising replacement for fetal bovine serum.
Mammalian cells are extensively used for production of biopharmaceuticals. Most cells used in industry have infinite proliferative capacity, which provides a high number of cells and corresponding productivity. However, infinite cells will continue to multiply even after cell density reaches sufficient levels.
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