Publications by authors named "Yasuhiro Kinugawa"
IgG4-related disease (IgG4-RD) is an autoimmune disease of unknown cause. is a transcription factor involved in immune responses, and its dysfunction leads to uncontrolled immune responses. We performed, to our knowledge, the first methylation analysis in type 1 autoimmune pancreatitis (denoted simply as AIP), a representative IgG4-RD.
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- The study focuses on ARL4C, a protein highly expressed in colorectal cancer (CRC), which is linked to increased cell mobility, invasion, and growth.
- Using a sensitive RNA detection method, researchers found that ARL4C was more prominently expressed at the invasion front of tumors and significantly associated with aggressive features like high-grade tumor budding and the epithelial-to-mesenchymal transition (EMT) phenotype.
- The findings suggest that higher levels of ARL4C in both cancer cells and stromal cells may indicate a poorer prognosis for CRC patients, highlighting the need for further research on ARL4C's role in cancer progression.
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- IgG4-positive plasma cells are found in increased numbers within the tumor microenvironment of various cancers, indicating a poor prognosis, but their role in intrahepatic cholangiocarcinoma (ICC) has not been thoroughly examined.
- This study analyzed 37 ICC patients who underwent surgery, assessing the presence of IgG and IgG4-positive plasma cells in different tumor regions, finding a strong correlation between high IgG4 presence in the invasion front and poor overall survival rates.
- The research concludes that high levels of IgG4 expression and the IgG4/IgG ratio serve as independent indicators of poor prognosis in ICC patients, suggesting that targeted IgG4 therapies could potentially enhance patient outcomes.
Article Synopsis
- LGR6 is a receptor linked to cancer progression, specifically studied in esophageal squamous cell carcinoma (ESCC) using RNAscope, a precise RNA detection method.
- In a study involving 41 ESCC cases, 37 showed LGR6 expression, with higher levels in aggressive tumors and those treated with neoadjuvant chemotherapy.
- High levels of LGR6 expression correlate with poorer patient prognosis, suggesting its potential as a prognostic indicator and a target for future therapies in ESCC.
Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is a known cancer stem cell marker. However, there are no reported analyses of LGR5 mRNA expression in normal liver and liver cancer tissues. Here, we evaluated LGR5 expression by RNAscope, a newly developed RNA in situ hybridization technique, using a tissue microarray consisting of 25 samples of intrahepatic cholangiocarcinoma (ICC) selected from the medical archives at our hospital.
View Article and Find Full Text PDFBackground: Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is a strong cancer stem cell marker in colorectal cancer; however, there are many unclear aspects of LGR5 expression in pancreatic cancer. It has been reported that the interaction between tumor cells and stroma at the fat infiltration site has a significant effect on pancreatic cancer prognosis. Therefore, we report a clinicopathological study of LGR5 expression at the fat invasion front in pancreatic cancer.
View Article and Find Full Text PDFBackground: Pancreaticobiliary maljunction (PBM) is a condition characterized by chronic inflammation due to refluxed pancreatic juice into the biliary tract that is associated with an elevated risk of biliary tract cancer. DNA double-strand breaks (DSBs) are considered the most serious form of DNA damage. DSBs are provoked by inflammatory cell damage and are recognized as an important oncogenic event in several cancers.
View Article and Find Full Text PDFBackground: It is difficult to distinguish between multicentric Castleman's disease (MCD) and IgG4-related lung disease (IgG4-LD), an IgG4-related disease (IgG4-RD) in the lung.
Methods: We focused on IL-6, which is elevated in MCD, to distinguish between MCD and IgG4-LD by RNAscope, a highly sensitive RNA in situ method. Six cases of MCD and four cases of IgG4-LD were selected.
Background: LGR5 is a promising stem cell marker in gastric pylorus, but there are few reports on its expression in human gastric corpus.
Aims: To investigate the involvement of LGR5 expression in gastric corpus ulcer regeneration in humans.
Methods: LGR5 expression was analyzed in five post-ESD ulcers during the healing process of regenerating epithelial cells of the gastric corpus.
Background: Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is an important cancer stem cell marker in gastric cancer. However, no detailed studies are available on LGR5 expression in poorly differentiated gastric adenocarcinoma (PD-AC). Therefore, we investigated the relationship between LGR5 expression and clinicopathological data in PD-AC.
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- Claudin 18 (CLDN18) is a cell surface protein involved in cell adhesion, with its isoform CLDN18.2 being highly expressed in gastric cancers, leading to the development of zolbetuximab for treating CLDN18.2-positive gastro-oesophageal adenocarcinoma.
- A study was conducted on 56 colitis-associated colorectal adenocarcinomas (CACs), revealing that 27% expressed CLDN18, which is significantly higher compared to only 5% in sporadic colorectal cancers, with a correlation to lymph node metastasis.
- The findings indicate that some CACs might be suitable for treatment with zolbetuximab, while only 4% showed HER2
LGR5 is expressed in various tumors and has been identified as a putative intestinal stem cell marker. Here we investigated LGR5 expression in colorectal neuroendocrine neoplasms and analyzed the correlation with pathological characteristics. We evaluated the clinicopathological features of 8 neuroendocrine tumor (NET) grade 1 (NET G1), 4 NET Grade 2 (NET G2), and 8 NET Grade 3 (NET G3; also termed neuroendocrine carcinoma, or NEC) cases.
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- The study investigates the connection between abnormal methylation of the SKI gene and autoimmune pancreatitis (AIP), a type of IgG4-related disease, using various analysis techniques.
- Findings show that SKI methylation is lower in AIP compared to cases of pancreatic ductal adenocarcinoma (PDA) and normal pancreas tissues, with higher expression levels in AIP.
- A significant correlation is noted between SKI methylation levels, IgG4 serum concentrations, and SKI expression, suggesting SKI may play a role in AIP pathogenesis, although its exact function remains unclear.
Objectives: Autoimmune pancreatitis (AIP) is a representative IgG4-related and inflammatory disease of unknown etiology. To clarify mechanisms of carcinogenesis resulting from AIP, we focused on methylation abnormalities and KRAS mutations in AIP.
Methods: Six tumor suppressor genes (NPTX2, Cyclin D2, FOXE1, TFPI2, ppENK, and p16) that exhibited hypermethylation in pancreatic carcinoma were selected for quantitative SYBR green methylation-specific polymerase chain reaction in 10 AIP specimens, 10 pancreatic adenocarcinoma cases without history of AIP containing carcinoma areas (CAs) and noncarcinoma areas (NCAs), and 11 normal pancreas (NP) samples.