Prevalence and molecular characterization of Acute hepatopancreatic necrosis disease (AHPND) in cultured white-leg shrimp Litopenaeus vannamei with the fungal bioactive control strategy.

Mass mortalities with clinical signs characteristic of acute hepatopancreatic necrosis disease (AHPND) were reported in cultured Litopenaeus vannamei from three Egyptian farms: Wadi-Mariote, Berket Ghalyoun, and Qarun Lake. During 4-month surveillance in 2023, shrimp samples were collected to investigate the prevalence of AHPND-causing Vibrio parahaemolyticus (Vp) based upon phenotypic identification, PCR, and DNA sequencing of pirA genes and pirB toxin genes followed by maximum likelihood phylogenetic analysis. In addition, the pathogenicity of V.