Isolation and differential transcriptome of vascular smooth muscle cells and mid-capillary pericytes from the rat brain.

Brain mural cells form a heterogeneous family which significantly contributes to the maintenance of the blood-brain barrier and regulation of the cerebral blood flow. Current procedures to isolate them cannot specifically separate their distinct subtypes, in particular vascular smooth muscle cells (VSMCs) and mid-capillary pericytes (mcPCs), which differ among others by their expression of smooth muscle actin (SMA). We herein describe an innovative method allowing SMA VSMCs and SMA mcPCs to be freshly isolated from the rat cerebral cortex.