Enzyme-induced Cu/Cu conversion as the electrochemical signal for sensitive detection of ethyl carbamate.

Traditional enzyme-linked immunosorbent assay (t-ELISA) method suffers from its relatively low sensitivity or accuracy in the detection of trace level of analyte in complicated samples. In this work, to extend the application of ELISA in practical samples, a newly electrochemical immunoassay (ECIA) was developed based on an enzyme-induced Cu/Cu conversion for the determination of ethyl carbamate (EC). Wherein, three rounds of signal transformation-the catalysis of ALP enzyme, the conversion of Cu/Cu and signal output of square wave voltammetry (SWV), can be realized to obtain higher sensitivity as compared to t-ELISA.

Nanozyme and aptamer- based immunosorbent assay for aflatoxin B1.

Traditional enzyme-linked immunosorbent assay (ELISA) suffers from the limitations of relatively low sensitivity and stability, and enzyme-labelled antibodies are hard to be prepared and purified. Based on a nanozyme, an aptamer and FeO magnetic nanoparticles (MNP), a nanozyme and aptamer-based immunosorbent assay (NAISA) was developed for aflatoxin B1 (AFB1) detection with simpler operation and separation. In this work, mesoporous SiO/Au-Pt (m-SAP) were prepared to act as signal labels, which showed high catalase-like activity and was denoted as nanozyme.

[Rapid Coal Classification Based on Confidence Machine and Near Infrared Spectroscopy].

Near-infrared reflectance spectroscopy (NIRS) is a simple, convenient and safe technology which is widely used in many industries. NIRS was employed to the rapid classification of coal in this study. The new method can be a replacement of the chemical analysis which is laborious and time consuming.