Publications by authors named "Yash Jawale"
Giant unilamellar vesicles (GUVs) are widely used as in vitro model membranes in biophysics and as cell-sized containers in synthetic biology. Despite their ubiquitous use, there is no one-size-fits-all method for their production. Numerous methods have been developed to meet the demanding requirements of reproducibility, reliability, and high yield while simultaneously achieving robust encapsulation.
View Article and Find Full Text PDFBackground: Rural India has a severe shortage of human resources for health (HRH). The National Rural Health Mission (NRHM) deploys HRH in the rural public health system to tackle shortages. Sanctioning under NRHM does not account for workload resulting in inadequate and inequitable HRH allocation.
View Article and Find Full Text PDFLabel-free imaging techniques such as differential interference contrast (DIC) allow the observation of cells and large subcellular structures in their native, unperturbed states with minimal exposure to light. The development of robust computational image-analysis routines is vital to quantitative label-free imaging. The reliability of quantitative analysis of time-series microscopy data based on single-particle tracking relies on accurately detecting objects as distinct from the background, i.
View Article and Find Full Text PDFArticle Synopsis
- Oscillatory gene circuits play key roles in biological processes like the cell cycle and circadian rhythms, and synthetic circuits are tested for understanding genetic network dynamics.
- The "repressilator" established the initial proof of concept, while a new dual-feedback oscillator combining lacI and araC loops offers enhanced control and robustness, modeled by a complex set of differential equations.
- A simplified model with six equations, including delay elements, helps explore the effects of gene expression asymmetry on oscillator behavior, confirming that variations in DNA copy number and translation rates significantly influence oscillation periods.