Publications by authors named "Yasemin Sucu"
Article Synopsis
- Current methods for diagnosing central nervous system (CNS) neoplasms using cerebrospinal fluid (CSF) cytology and flow cytometry are often inadequate, as these conditions can resemble infections or autoimmune diseases.
- The study aimed to evaluate the effectiveness of metagenomic next-generation sequencing (mNGS) for identifying aneuploidy in challenging cases of CNS malignant tumors.
- Two case-control studies at UCSF included a total of 130 participants, assessing mNGS performance compared to traditional testing, and sought to determine the sensitivity and specificity of detecting chromosomal abnormalities in patients with suspected neuroinflammatory conditions that turned out to be neoplasms.
We developed a metagenomic next-generation sequencing (mNGS) test using cell-free DNA from body fluids to identify pathogens. The performance of mNGS testing of 182 body fluids from 160 patients with acute illness was evaluated using two sequencing platforms in comparison to microbiological testing using culture, 16S bacterial PCR and/or 28S-internal transcribed ribosomal gene spacer (28S-ITS) fungal PCR. Test sensitivity and specificity of detection were 79 and 91% for bacteria and 91 and 89% for fungi, respectively, by Illumina sequencing; and 75 and 81% for bacteria and 91 and 100% for fungi, respectively, by nanopore sequencing.
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