Frequency of positive donor rim cultures after penetrating keratoplasty using hypothermic and organ-cultured donor corneas.

Purpose: To compare the frequency of positive rim cultures after penetrating keratoplasty using corneas preserved by hypothermic and organ culture storage. To evaluate the influence of standard procurement techniques on the frequency of microbial donor rim contamination.

Methods: Six hundred four donor corneas stored at 31 degrees C and 214 at 4 degrees C were studied.