[Jinshuibao capsule combined losartan potassium intervened early renal damage of hypertension patients of yin and yang deficiency: a clinical research].

Objective: To observe the effects of Jinshuibao Capsule (JC) combined losartan potassium on some indices of early renal damage of hypertension patients of yin and yang deficiency syndrome (YYDS), such as levels of serum cystatin C (Cys C), beta2-microglobulin (beta2-MG), hypersensitive C-reactive protein (hs-CRP), uric acid (UA), blood pressure, blood lipids, and fasting blood glucose (FBG), and to explore their protective effects on early renal damage of hypertension patients and on the metabolisms of blood lipids and blood glucose.

Methods: Totally 106 hypertension patients of YYDS were randomly assigned to two groups, 53 patients in the control group (treated by losartan potassium) and 53 patients in the treatment group (treated by JC + losartan potassium). The treatment lasted for 16 weeks.

Association between reversal of multidrug resistance by methyl jasmonate and P-glycoprotein ATPase activity in hepatocellular carcinoma.

Objective: To study the effects of methyl jasmonate on multidrug resistance in a mouse model of hepatocellular carcinoma.

Methods: Multidrug resistant H22 (H22/FAP) hepatocellular carcinoma cells were produced in vitro by continuous exposure to increasing doses of doxorubicin, cisplatin and 5-fluorouracil (FAP regimen). Cell toxicity was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolum bromide (MTT) assay.

Poly[[tetrachlorido{μ4-tetrakis[(pyridin-4-yl)oxymethyl]methane-κ4N:N':N'':N'''}dizinc(II)] dimethylformamide tetrasolvate].

A novel metal-organic framework, {[Zn2Cl4(C25H24N4O4)]·4C3H7NO}n, has been synthesized solvothermally by assembling the semi-rigid tetrahedral ligand tetrakis[(pyridin-4-yl)oxymethyl]methane (tpom) and zinc nitrate in dimethylformamide (DMF). The crystal structure is noncentrosymmetric (P42(1)c). Each Zn(II) cation has a tetrahedral coordination environment (C2 symmetry), which is formed by two chloride ligands and two pyridine N atoms from two tpom ligands.

Identification of the proteins in green cotton fiber using a proteomics-based approach.

The proteome of 21 DPA (days post-anthesis) of green cotton fiber was analyzed using two-dimensional gel electrophoresis to yield the first reference proteome map. Of 220 individual spots that were excised and analyzed by MALDI-TOF/TOF MS, 156 were identified and cataloged according to their functions. Twelve classes of proteins were identified.

The membrane fouling characteristics of MBRs with different aerobic granular sludges at high flux.

This experimental work investigated the property of membrane fouling for different sludges at high flux 20 L/(m(2)h). The MBR with good aerobic granular sludge performed the longest operation time 61 days, and TMP rose up in a steady overall rate, while only 10, 14 and 19 days for bulking, flocculent and small granular sludge, respectively, which clearly demonstrated the good and complete aerobic granules greatly retarded the membrane fouling. The pore blocking resistance 76.

Tumor necrosis factor-α, monocyte chemoattractant protein-1 and intercellular adhesion molecule-1 increase during the development of a 2,4-dinitrofluorobenzene-induced immediate-type dermatitis in rats.

Background: Due to the steadily increasing incidence of atopic dermatitis, there is a great medical need for new therapies and improved animal models.

Objective: To provide more detailed analysis of a Sprague-Dawley rat dermatitis model.

Methods: Sprague-Dawley rats were actively sensitized by intraperitoneal injections of dinitrophenylated ovalbumin (DNP-OVA) plus alum.

Selective fluorescence response and magnetic separation probe for 2,4,6-trinitrotoluene based on iron oxide magnetic nanoparticles.

Despite the rapid development of nanomaterials and nanotechnology, it is still desirable to develop novel nanoparticle-based techniques which are cost-effective, timesaving, and environment-friendly, and with ease of operation and procedural simplicity, for assay of target analytes. In the work discussed in this paper, the dye fluorescein isothiocyanate (FITC) was conjugated to 1,6-hexanediamine (HDA)-capped iron oxide magnetic nanoparticles (FITC-HDA Fe3O4 MNPs), and the product was characterized. HDA ligands on the surface of Fe3O4 MNPs can bind 2,4,6-trinitrotoluene (TNT) to form TNT anions by acid-base pairing interaction.

High-rate nitrogen removal and its behavior of granular sequence batch reactor under step-feed operational strategy.

Alternating anoxic/oxic (A/O) combined with the step-feed granular sequence batch reactor (step-feed SBR) was operated in laboratory scale to investigate nitrogen removal. The results showed that when the total inorganic nitrogen (TIN) and chemical oxygen demand (COD) levels were 55 and 320 mg/L in the influent, the TIN removal efficiencies were 89.7-92.

[Analyses of karyotypic characteristics and prognosis in pediatric acute myeloblastic leukemia].

Objective: Acute myeloblastic leukemia (AML) accounts for 15 to 25 percent of childhood acute leukemias. Cytogenetic information is important for diagnosis, classification and prognosis of AML. Our aim was to analyze the relationship between karyotypic characteristics and prognosis of childhood AML.

[Determination of patulin in juice by ultra performance liquid chromatography-tandem mass spectrometry].

A method was developed for the simultaneous determination of patulin in juice by solid phase extraction (SPE)-ultra performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). The cloudy juice was hydrolyzed by enzyme and extracted by ethyl acetate. The extract of cloudy juice was then enriched and purified by an HLB SPE cartridge.

[Analysis of DNMT3a mutation in childhood acute myeloid leukemia].

Within the past few years, the invention of next-generation sequencing has revealed several new genes associated with tumor formation and development, for example DNMT3a. This gene is an independent prognostic factor for acute myeloid leukemia (AML). The objective of this study was to analyze the DNMT3a mutation in childhood AML in a single center.

Evolutionary expansion and functional diversification of oligopeptide transporter gene family in rice.

Background: Oligopeptide transporters (OPTs) play important roles in the mobilization of organic nitrogenous compounds and usually associate with tissues that show signs of rapid protein hydrolysis, such as germinating seeds and senescing leaves. This study is to investigate rice OPT genes.

Results: A total of sixteen OsOPT genes (Os for Oryza sative L.

[Ovarian torsion after controlled ovarian hyperstimulation: 5 cases report and clinical analysis].

Objective: To evaluate the characteristics and treatment of ovary torsion after controlled ovarian hyperstimulation.

Methods: Between Jan.2008 and Dec.

[Diagnostic value of fluorescence in situ hybridization for children with acute myeloid leukemia].

This study was purposed to investigate the diagnostic value of fluorescence in situ hybridization (FISH) technique for the childhood acute myeloid leukemia (AML). The medical data of 179 children with AML (aged ≤ 16 years) were retrospectively studied, who were initially diagnosed in our hospital from April 2005 to April 2010. Through the analysis of the results of FISH, chromosome banding analysis and polymerase chain reaction, the difference and complementarity between FISH and other 2 methods for detecting the fusion genes were explored.

Characterization of sequence elements from Malvastrum yellow vein betasatellite regulating promoter activity and DNA replication.

Background: Many monopartite begomoviruses are associated with betasatellites, but only several promoters from which were isolated and studied. In this study, the βC1 promoter from Malvastrum yellow vein betasatellite (MYVB) was characterized and important sequence elements were identified to modulate promoter activity and replication of MYVB.

Results: A 991 nucleotide (nt) fragment upstream of the translation start site of the βC1 open reading frame of MYVB and a series of deletions within this fragment were constructed and fused to the β-glucuronidase (GUS) and green fluorescent protein (GFP) reporter genes, respectively.

Progesterone production requires activation of caspase-3 in preovulatory granulosa cells in a serum starvation model.

Granulosa cells proliferate, differentiate, and undergo apoptosis throughout follicular development. Previous studies have demonstrated that stimulation of progesterone production is accompanied by caspase-3 activation. Moreover, we previously reported that arsenic enhanced caspase-3 activity coupled with progesterone production.

Matrix solid-phase dispersion extraction for chromatographic analysis of labdane diterpenoids in Coleus forskohlii.

Introduction: The quality of Coleus forskohlii is often evaluated by high performance liquid chromatography (HPLC), using bioactive labdane diterpenoids as chemical markers. However, the existing sample preparation methods for the analysis of diterpenoids in C. forskohlii are generally labour-intensive, time-consuming and require large volumes of solvents.

[Analysis of clinical characteristics in 45 cases of Diamond-Blackfan anemia].

In order to explore the diagnosis and therapy of Diamond Blackfan anemia (DBA), the clinical data of 45 cases of DBA admitted in our hospital from February 1994 to July 2011 were analyzed retrospectively. The clinical characteristics, results of laboratory examination, treatment reaction and outcome of disease were investigated. The results indicated that out of 45 children diagnosed as DBA, 14 cases (31.

Increased platelet volume in a general population with prehypertension: a cross-sectional study of 80 545 participants from China.

Mean platelet volume (MPV), an indicator of platelet activation, has been shown to be elevated in patients with hypertension. However, data available on the association between MPV level and prehypertension are limited. Prehypertension is also associated with an increase in cardiovascular morbidity and mortality.

Safety and efficacy of adalimumab for moderate to severe Crohn's disease in children.

Background & Aims: The IMAgINE 1 study (NCT00409682) evaluated the safety and efficacy of adalimumab double-blind maintenance dosing regimens following open-label induction for pediatric patients with moderate to severe Crohn's disease (CD).

Methods: We studied 192 patients with Pediatric Crohn's Disease Activity Index (PCDAI) scores >30 for whom conventional treatment was unsuccessful. Patients received open-label induction therapy with subcutaneous adalimumab at weeks 0 and 2 (160 mg and 80 mg, or 80 mg and 40 mg, for body weight ≥40 kg or <40 kg).

Electrogenerated chemiluminescence biosensor incorporating ruthenium complex-labelled Concanavalin A as a probe for the detection of Escherichia coli.

A novel electrogenerated chemiluminescence (ECL) biosensor for highly sensitive detection of Escherichia coli (E. coli) was first developed by employing Concanavalin A (Con A) as a biological recognition element and bis(2,2'-bipyridine)-4'-methyl-4-carboxybipyridine ruthenium (II) (Ru1) complex as the detector. The ECL biosensor was fabricated by adsorbing carboxyl-functionalised single-wall carbon nanotubes (SWNTs) onto a paraffin-impregnated graphite electrode and further covalently coupling the Ru1-Con A probe onto the surface of the SWNT-modified electrode.

AtACDO1, an ABC1-like kinase gene, is involved in chlorophyll degradation and the response to photooxidative stress in Arabidopsis.

ABC1 (activity of bc1 complex) is a newly discovered atypical kinase in plants. Here, it is reported that an ABC1 protein kinase-encoded gene, AtACDO1 (ABC1-like kinase related to chlorophyll degradation and oxidative stress), located in chloroplasts, was up-regulated by methyl viologen (MV) treatment. AtACDO1 RNAi (RNA interference) plants showed developmental defects, including yellow-green leaves and reduced contents of carotenoids and chlorophyll; the chlorophyll reduction was associated with a change in the numbers of chlorophyll-binding proteins of the photosynthetic complexes.

Lower serum UA levels in Parkinson's disease patients in the Chinese population.

Parkinson's disease (PD) is the second most common neurodegenerative disease in the world, and oxidative stress plays an important role in its pathogenesis. Uric acid (UA) is a product of purine metabolism and is a natural antioxidant that can relieve the oxidative stress that occurs in PD. Recent studies have indicated that the serum UA level are associated with a risk of PD and PD progression of motor symptoms and have proposed UA as a possible biomarker of the underlying pathophysiology of PD.

OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice.

The microRNA miR393 has been shown to play a role in plant development and in the stress response by targeting mRNAs that code for the auxin receptors in Arabidopsis. In this study, we verified that two rice auxin receptor gene homologs (OsTIR1 and OsAFB2) could be targeted by OsmiR393 (Os for Oryza sativa). Two new phenotypes (increased tillers and early flowering) and two previously observed phenotypes (reduced tolerance to salt and drought and hyposensitivity to auxin) were observed in the OsmiR393-overexpressing rice plants.