Simulation, Design, and Test of a Dual-Differential D-Dot Overvoltage Sensor Based on the Field-Circuit Coupling Method.

Accurate measurement of overvoltage in power grids is of great significance to study the characteristics of overvoltage and design of insulation coordination. Based on the research of D-dot voltage sensor, we designed a Dual-Differential D-dot overvoltage sensor. In order to quantify the structural parameters of the sensor, improve the performance and measurement accuracy of the sensor.

Inhibition of HMGB1 improves necrotizing enterocolitis by inhibiting NLRP3 via TLR4 and NF-κB signaling pathways.

Objective: To explore the relationship between high-mobility group box 1 (HMGB1) and NLR pyrin domain containing 3 (NLRP3) in the development of necrotizing enterocolitis (NEC).

Methods: NEC rat models were constructed and treated with HMGB1 inhibitor glycyrrhizin (GL) with different concentration. An inflammatory condition of intestinal tissue in newborn NEC rats was observed by hematoxylin and eosin staining.

Dynamic sandwich-type electrochemical assay for protein quantification and protein-protein interaction.

The study of protein-protein interactions (PPIs) plays an important role in the understanding of biological systems; however, the established methods for PPI analysis often involve cumbersome sample preparation, multiple detecting steps, and costly instruments. Here we report a versatile and sensitive electrochemical method based on PPI-induced distinctive migration behavior of DNA deoxyribozyme (DNAzyme) on an electrode surface. In this method, the cleavage activity of DNAzyme toward the substrate DNA modified on the electrode surface is inversely correlated with the hydrodynamic diameter of the macromolecule attached to it.

Flexible regulation of DNA displacement reaction through nucleic acid-recognition enzyme and its application in keypad lock system and biosensing.

Toehold-mediated DNA strand displacement reaction (SDR) plays pivotal roles for the construction of diverse dynamic DNA nanodevices. To date, many elements have been introduced into SDR system to achieve controllable activation and fine regulation. However, as the most relevant stimuli for nucleic acid involved reaction, nucleic acid-recognizing enzymes (NAEs) have received nearly no attention so far despite SDR often takes place in NAEs-enriched environment (i.

In Vitro Analysis of DNA-Protein Interactions in Gene Transcription Using DNAzyme-Based Electrochemical Assay.

The interaction between protein and DNA elements controls a variety of functions of genomes. The development of a convenient and cost-effective method for investigating the sequence specificity of DNA-binding proteins represents an important challenge. In response, we have introduced an electrochemical assay in this work for specific and sensitive analysis of interaction between protein and nucleic acid in nucleic extracts, based on the protein-induced distinctive motion behavior of DNA deoxyribozyme (DNAzyme) on an electrode surface.

Simple and fast screening of G-quadruplex ligands with electrochemical detection system.

Small molecules that may facilitate and stabilize the formation of G-quadruplexes can be used for cancer treatments, because the G-quadruplex structure can inhibit the activity of telomerase, an enzyme over-expressed in many cancer cells. Therefore, there is considerable interest in developing a simple and high-performance method for screening small molecules binding to G-quadruplex. Here, we have designed a simple electrochemical approach to screen such ligands based on the fact that the formation and stabilization of G-quadruplex by ligand may inhibit electron transfer of redox species to electrode surface.

Electrochemical detection of Nanog in cell extracts via target-induced resolution of an electrode-bound DNA pseudoknot.

Nanog is among the most important indicators of cell pluripotency and self-renew, so detection of Nanog is critical for tumor assessment and monitoring of clinical prognosis. In this work, a novel method for Nanog detection is proposed by using electrochemical technique based on target-induced conformational change of an electrode-bound DNA pseudoknot. In the absence of Nanog, the rigid structure of the pseudoknot will minimize the connection between the redox tag and the electrode, thus reducing the obtained faradaic current.

Dynamic light scattering (DLS)-based immunoassay for ultra-sensitive detection of tumor marker protein.

A novel dynamic light scattering (DLS)-based immunoassay that utilizes manganese dioxide nanosheet-modified gold nanoparticles (MnO2-GNPs) as an activatable nanoprobe has been developed to detect tumor markers down to femtomolar levels.

Inertial measurements of upper limb motion.

We present an inertial sensor based monitoring system for measuring upper limb movements in real time. The purpose of this study is to develop a motion tracking device that can be integrated within a home-based rehabilitation system for stroke patients. Human upper limbs are represented by a kinematic chain in which there are four joint variables to be considered: three for the shoulder joint and one for the elbow joint.