Enhancing Immune Responses through Modulation of Innate Cell Microenvironments in Lymph Nodes with Virus-Mimetic Vaccines.

Nanovaccines hold significant promise for the prevention and treatment of infectious diseases. However, the efficacy of many nanovaccines is often limited by inadequate stimulation of both innate and adaptive immune responses. Herein, we explore a rational vaccine strategy aimed at modulating innate cell microenvironments within lymph nodes (LNs) to enhance the generation of effective immune responses.

Astragalin actives autophagy and inhibits apoptosis of astrocytes in AD mice via down-regulating Fas/Fasl-VDAC1 pathway.

Alzheimer's disease (AD) as a common neurodegenerative disease, which characterized by amyloid Aβ deposition and neurofibrillary tangles. Astragalin (AST), a natural flavonoid, has anti-inflammatory, antioxidant, anti-cancer, and other pharmacological effects. Astrocytes can phagocytize and degrade Aβ in their vicinity.

Astragalin relieves inflammatory pain and negative mood in CFA mice by down-regulating mGluR5 signaling pathway.

As a flavonoid compound, astragalin (AST) is found in a variety of medicinal plants. In clinical studies, AST has anti-inflammatory and analgesia effects on rheumatoid arthritis, bronchopneumonia diseases and so on, but its specific role and mechanism is still not clear. This study aimed to investigate the effect and molecular mechanism of AST on inflammatory pain and pain-related emotions in complete Freund's adjuvant (CFA) mice.

Molecular Subtyping and Therapeutic Targeting of IFNG-Driven Immunogenic Cell Death in Lung Adenocarcinoma.

Background: Immunogenic cell death (ICD) can be triggered by various therapies to induce anti-tumor immune responses, significantly enhancing treatment effectiveness, and is widely utilized in tumor immunotherapy.

Methods: LUAD data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) validated ICD-related molecular subtypes via consensus clustering. Clinical features, ICD genes, driver genes, mutations, tumor microenvironment, immune checkpoints, and drug sensitivity were compared.

Study on forensic diagnostic biomarker combination for acute ischemia heart disease based on postmortem biochemistry.

Sudden cardiac death (SCD) caused by ischemia heart disease (IHD) has traditionally been identified through autopsy and microscopic examination. However, these methods are limited in diagnosing cases of acute ischemia heart disease (AIHD) without typical myocardial necrosis and excessive reliance on the subjective experience of experts. Postmortem biochemistry is crucial in the assisted diagnosis of SCD in forensic medicine.

Astragalin inhibits neuronal excitability and activates neuronal autophagy in the ACC and LH of CFA mice to alleviate inflammatory pain and pain-related emotions.

Astragalin (AST), a natural flavonoid, exhibits anti-inflammatory, anti-cancer, and antioxidant properties. However, its effects and molecular mechanisms in inflammatory pain remain unclear. Therefore, this study aims to investigate the impact of AST on a Complete Freund's Adjuvant (CFA)-induced inflammatory pain mouse model and to elucidate its potential mechanisms.

Multidimensional Resonance Controlled by Critical Size in Printed Binary Colloidal Crystals for High-Contrast Imaging.

Colloidal crystal engineering enables the precise construction of structures with remarkable properties. However, the flexible and synergistic regulation of multiple properties of colloidal crystals remains a significant challenge. Here, we inspire from Brazilian opals to self-assemble polymer nanoparticles in the gaps of a single-layer opal substrate to fabricate large-scale binary colloidal crystals (BCCs).

The effects of interactive video games combined with LEGO game therapy on social anxiety in rural left-behind children.

Background: China has a significant population of left-behind children, defined as those whose parents have migrated away from their home for at least six months. This situation arises when one or both parents leave to seek work in urban areas. Among the various challenges faced by left-behind children, social anxiety has emerged as one of the most pressing issues.

DNAJC12 downregulation induces neuroblastoma progression via increased histone H4K5 lactylation.

Neuroblastoma (NB) is the most common extracranial solid tumor in children. Despite treatment advances, the survival rates of high-risk NB patients remain low. This highlights the urgent need for a deeper understanding of the molecular mechanisms driving NB progression to support the development of new therapeutic strategies.

GW117 induces anxiolytic effects by improving hippocampal functions.

GW117 functions as both an MT1/MT2 receptor agonist and a 5-HT2C receptor antagonist. This study aimed to investigate the anxiolytic effects of GW117 through behavioral assessments, including the open field test and novelty-suppressed feeding test (NSFT) within a chronic unpredictable mild stress (CUMS) model. GW117 was administered via oral gavage for 21 days to evaluate its sustained anxiolytic effects, with behavioral tests including the NSFT, the Vogel-conflict test, and the O-maze test.

Predictive cytokines of omalizumab in the treatment of chronic spontaneous urticaria.

Background: Omalizumab, an anti-IgE biological agent, is commonly prescribed as a second-line therapy for Chronic Spontaneous Urticaria (CSU). However, there is a lack of biomarkers to predict which CSU patients will respond favorably to omalizumab.

Objective: Our study aims to identify cytokine markers associated with the efficacy of omalizumab in treating CSU.

Ag@CDS SERS substrate coupled with lineshape correction algorithm and BP neural network to detect thiram in beverages.

Surface enhanced Raman scattering (SERS) has been proved an effective analytical technique due to its high sensitivity, however, how to identify and extract useful information from raw SERS spectra is still a problem that needs to be resolved. In this work, a composite SERS substrate was prepared by encapsulating Ag nanoparticles within dialdehyde starch (Ag@CDS) to obtain dense "hot spot", and then a novel spectral preprocessing algorithm namely lineshape correction algorithm (LCA) was developed to separate the characteristic peaks of analytes from the original SERS spectra. Based on Ag@CDS and LCA, thiram residues in different beverages were quantitatively detected using back propagation (BP) neural network regression model.

Adverse childhood experiences and multimorbidity among middle-aged and older adults: Evidence from China.

Background: Associations between adverse childhood experiences (ACEs) and multimorbidity among middle-aged and older Chinese individuals have not been well documented.

Objective: We aimed to examine the associations of ACEs and different multimorbidity measures among Chinese middle-aged and older adults.

Participants And Setting: The study population included 6428 participants aged 45 years or older who were obtained from the China Health and Retirement Longitudinal Study (CHARLS).

Effects of atropine on choroidal thickness in myopic children: a meta-analysis.

Background: Atropine is an effective medicine for myopia prevention and control. This meta-analysis was conducted to investigate the effects of atropine on choroidal thickness (ChT) in children with myopia.

Methods: Between its inception and 1 June 2023, Medline, Embase, and Web of Science were all searched, and only English literature was included.

Overexpression of FBP1 enhances dendritic cell activation and maturation by inhibiting glycolysis and promoting the secretion of IL33 in lung adenocarcinoma.

Fructose 1,6-diphosphatase 1 (FBP1) is an enzyme involved in gluconeogenesis and glycolysis inhibition. Dendritic cells (DCs) are antigen-presenting cells, and antigens presented to T cells activate the immune response. FBP1 inhibits the development of several tumors, and high FBP1 expression inhibits the proliferation, migration, and invasion of lung cancer cells.

Determining the sequence of intersecting lines formed by laser printer toner and seal ink based on confocal Raman spectroscopy.

In the field of questioned document examination, determining the sequence of intersecting lines is still a technical challenge. This study aims to validate whether confocal Raman spectroscopy can determine the sequence of intersecting lines created by laser prints toner and seal ink through empirical research. The study collected 110 varieties of seal ink and 1074 test pages from 66 models of laser printers available in the Chinese market.

Deubiquitinating enzyme USP28 inhibitor AZ1 alone and in combination with cisplatin for the treatment of non-small cell lung cancer.

Lung cancer is one of the most common malignant tumors. Despite decades of research, the treatment of lung cancer remains challenging. Non-small cell lung cancer (NSCLC) is the primary type of lung cancer and is a significant focus of research in lung cancer treatment.

Nanosized-laser-induced sub-20 nm homogenous alloy nanoparticles.

Alloy nanoparticles (NPs) have great potential in nanosized 3D-printing, surface coating, plasmonic enhancement, information coding, and so forth. However, chemical-pollution-free and homogeneous sub-20 nm NPs maintain still a challenge in preparation. Here we present a smart nanosecond laser scan strategy of alloy-NPs preparation on a bilayer metal film by using a nanosized focused beam, successfully realizing controllable fabrication of the sub-20 nm homogeneous alloy NPs without pollution.

2D/3D hierarchical porous structure of mNPC/SMOH@C to construct an electrochemical sensor for the simultaneous determination of p-acetylaminophenol and p-aminophenol.

Background: As persistent organic pollutants (POPs), the accumulation of p-acetylaminophenol (PAT) and p-aminophenol (PAP) in water can seriously damage the health of plants and animals, ultimately leading to threats to human health and safety. Electrochemical sensors have the advantages of being fast, inexpensive, and accurate compared to the complex, expensive, and cumbersome conventional analytical methods. In this study, we designed and synthesized composites with two-dimensional/three-dimensional (2D/3D) porous structures to construct an efficient electrochemical platform for the simultaneous detection of PAT and PAP.

Augmented type 2 inflammatory response in allergic rhinitis patients experiencing systemic reactions to house dust mite subcutaneous immunotherapy.

Background: Subcutaneous immunotherapy (SCIT) can induce systemic reactions (SRs) in certain patients, but the underlying mechanisms remain to be fully elucidated.

Methods: AR patients who were undergoing standardized HDM SCIT (Alutard, ALK) between 2018 and 2022 were screened. Those who experienced two consecutive SRs were included in the study group.

Ferroptosis: a novel mechanism of cell death in ophthalmic conditions.

Ferroptosis, a new type of programmed cell death proposed in recent years, is characterized mainly by reactive oxygen species and iron-mediated lipid peroxidation and differs from programmed cell death, such as apoptosis, necrosis, and autophagy. Ferroptosis is associated with a variety of physiological and pathophysiological processes. Recent studies have shown that ferroptosis can aggravate or reduce the occurrence and development of diseases by targeting metabolic pathways and signaling pathways in tumors, ischemic organ damage, and other degenerative diseases related to lipid peroxidation.

Peptide-TLR7/8a-Coordinated DNA Vaccines Elicit Enhanced Immune Responses against Infectious Diseases.

DNA vaccines represent an innovative approach for the immunization of diverse diseases. However, their clinical trial outcomes are constrained by suboptimal transfection efficiency and immunogenicity. In this work, we present a universal methodology involving the codelivery of Toll-like receptor 7/8 agonists (TLR7/8a) and antigen gene using TLR7/8a-conjugated peptide-coated poly(β-amino ester) (PBAE) nanoparticles (NPs) to augment delivery efficiency and immune response.