Publications by authors named "Yaozhou Wu"
Article Synopsis
- This study combines CRISPR/Cas12a technology with Multiply-primed-RCA (MRCA) for improved point-of-care testing (POCT) for tuberculosis, addressing limitations in current diagnostic methods.
- Researchers optimized the method using different DNA ligases and primers, ultimately creating two effective testing methods (T4-MRCA-Cas12a and Taq-MRCA-Cas12a) that can be performed in a single tube.
- The testing methods showed high accuracy and specificity, effectively detecting tuberculosis with no interference from other bacterial DNA, allowing for results to be observed visually.
Article Synopsis
- * By optimizing primer combinations and ratios, the research enhances the amplification efficiency of the newly developed nested Multi-AS-PCR-Cas12a method, achieving high sensitivity and specificity in mutation detection.
- * This innovative technique could be widely applicable for identifying drug-resistant mutations in infectious diseases and cancers, as well as for screening genetic disorders.
Background: The increasing prevalence of fungal infections necessitates broader use of antifungal medications. However, the prevalence of adverse drug events (ADEs) restricts their clinical application. This study aimed to develop a reliable ADEs trigger for antifungals to enable proactive ADEs monitoring, serving as a reference for ADEs prevention and control.
View Article and Find Full Text PDFPurpose: Accurate detection and identification of pathogens and their associated resistance mechanisms are essential prerequisites for implementing precision medicine in the management of Carbapenem-resistant (CRE). Among the various resistance mechanisms, the production of KPC carbapenemase is the most prevalent worldwide. Consequently, this study aims to develop a convenient and precise nucleic acid detection platform specifically for the gene.
View Article and Find Full Text PDFThe clinical characteristics and risk factors of infusion reactions (IRs) are inadequately described in clinical practice due to underreported cases. In the present study, we reported the current status of IRs based on an in-hospital pharmacovigilance database of a tertiary care hospital. Our study conducted a retrospective analysis of drug-induced IRs recorded at an in-hospital pharmacovigilance center between January 2015 to December 2019.
View Article and Find Full Text PDFThe use of MV can easily lead to VAP especially in ICU patients. SUP, sedatives, statin and insulin have been proved to prevent VAP and improve the prognosis of patients. Our aim was to analyze the effects of SUP, sedative, statin, and insulin on patients with MV.
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