Mater Sci Eng C Mater Biol Appl
July 2013
In order to develop the nutritional trace elements which could be absorbed and utilized effectively, protein chelates were adopted. Calcium, copper and manganese were considered based on their physiological functions, and the new chelates of HLC-Ca, HLC-Cu and HLC-Mn were formed in MOPS or MES buffer and purified by gel chromatography, and then freeze-dried. And they were detected and analyzed by atomic absorption spectrophotometry, ultraviolet-visible absorption (UV-vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, fluorescence quenching method, circular dichroism (CD) and differential scanning calorimetry (DSC).
View Article and Find Full Text PDFNext-generation sequencing is increasingly employed in biomedical investigations. Strong concordance between microarray and mRNA-seq levels has been reported in high quality specimens but information is lacking on formalin-fixed, paraffin-embedded (FFPE) tissues, and particularly for microRNA (miRNA) analysis. We conducted a preliminary examination of the concordance between miRNA-seq and cDNA-mediated annealing, selection, extension, and ligation (DASL) miRNA assays.
View Article and Find Full Text PDFCombining Co2 clusters with mixed H2bpdc and H2bpz ligands creates an unprecedented 3-fold interpenetrated 5-connected porous framework, which possesses the fascinating double-walled nanotubes structure assembled from nonuple and triple helices, and exhibits gas sorption properties.
View Article and Find Full Text PDFPurpose: To develop a standardized approach for molecular diagnostics, we used the gene expression ratio bioinformatic technique to design a molecular signature to diagnose malignant pleural mesothelioma (MPM) from among other potentially confounding diagnoses and differentiate the epithelioid from the sarcomatoid histologic subtype of MPM. In addition, we searched for pathways relevant in MPM in comparison with other related cancers to identify unique molecular features in MPM.
Experimental Design: We conducted microarray analysis on 113 specimens including MPMs and a spectrum of tumors and benign tissues comprising the differential diagnosis of MPM.
A Zn(II)-MOF [Zn(2)(tzba)(DMF)]·0.5DMF (1) (H(2)tzba = 4-(1H-tetrazol-5-yl) benzoic acid, DMF = N,N'-dimethylformamide) has been solvothermally synthesized and characterized. 1 is a three-dimensional (3D) microporous framework possessing an unusual multi-nodal (3,3,6)- or (4,4,4,4)-connected topological net.
View Article and Find Full Text PDFBackground: Short-term changes in preoperative nutrition can have profound effects on surgery-related outcomes such as ischemia/reperfusion injury in preclinical models. Dietary interventions that lend protection against stress in animal models (eg, fasting, dietary restriction [DR]) impact adipose tissue quality/quantity. Adipose tissue holds high surgical relevance because of its anatomic location and large tissue volume, and it is ubiquitously traumatized during surgery.
View Article and Find Full Text PDFTo investigate the conformation of cyclopentanetetracarboxylic acid, four new "brick-wall"-like metal-organic frameworks have been synthesized from hydrothermal reactions with different metal salts, (S,S,R,R)-1,2,3,4-cyclopentanetetracarboxylic acid (H(4)cptc) and auxiliary N-donor ligands, namely, Cu(2)(S,S,R,R-cptc)(bpe)(H(2)O)(2)·2H(2)O (1), Co(2)(S,S,R,R-cptc)(bpe)(0.5)(H(2)O)(2)·2H(2)O (2), Cd(4)(S,S,R,R-cptc)(2)(bpa)(2)(H(2)O)(5)·2H(2)O (3) and Co(2)(S,S,R,R-cptc)(bpy)(0.5)(H(2)O)(2)·2(H(2)O) (4) (bpe = 4-(2-(pyridine-4-yl)vinyl)pyridine, bpa = 4-(2-(pyridine-4-yl)ethyl)pyridine, bpy = 4-(pyridine-4-yl)pyridine).
View Article and Find Full Text PDFMolecular braids represent one of the most fascinating topological networks in the fields of supramolecular chemistry and crystal engineering. In this Tutorial Review we provide an overview of the sustainable exploration of those metal-organic frameworks (MOFs) with the topology of molecular braid. Since the first example of the triple-stranded molecular braid in MOFs was reported by our group in 2005, another five attractive molecular braids in MOFs have already been prepared through several different pathways by us and other groups.
View Article and Find Full Text PDFA novel and general procedure for Cu-catalyzed coupling of enamides with alkynes to synthesize substituted pyridines was developed. The chemistry was allowed to extend to the synthesis of substituted isoquinolines by coupling of enamides with arynes under transition-metal-free conditions.
View Article and Find Full Text PDFPurpose: The disease course of chronic lymphocytic leukemia (CLL) varies significantly within cytogenetic groups. We hypothesized that high-resolution genomic analysis of CLL would identify additional recurrent abnormalities associated with short time-to-first therapy (TTFT).
Experimental Design: We undertook high-resolution genomic analysis of 161 prospectively enrolled CLLs using Affymetrix 6.
Deep sequencing technologies have the potential to transform the study of highly variable viral pathogens by providing a rapid and cost-effective approach to sensitively characterize rapidly evolving viral quasispecies. Here, we report on a high-throughput whole HIV-1 genome deep sequencing platform that combines 454 pyrosequencing with novel assembly and variant detection algorithms. In one subject we combined these genetic data with detailed immunological analyses to comprehensively evaluate viral evolution and immune escape during the acute phase of HIV-1 infection.
View Article and Find Full Text PDFHigh-quality cobalt-doped ceria nanostructures with triangular column, triangular slab, and disklike shapes are synthesized by tuning the doping amount of cobalt nitrate in a facile hydrothermal reaction. The cobalt-doped ceria nanodisks display significantly enhanced catalytic activity in CO oxidation due to exposed highly active crystal planes and the presence of numerous surface defects.
View Article and Find Full Text PDFA new dynamic porous coordination polymer (PCP) [Ni(dcpy)(bipy)(0.5)(H(2)O)]·1.5H(2)O (1) was synthesized by assembly of 3-(2',5'-dicarboxylphenyl)pyridine (dcpy), 4,4'-bipyridine (bipy) and NiSO(4)via solvothermal, hydrothermal and microwave methods, displaying a wavelike 2D stacked layer framework.
View Article and Find Full Text PDFBackground: Oral pathology is a commonly reported extraintestinal manifestation of Crohn's disease (CD). The host-microbe interaction has been implicated in the pathogenesis of inflammatory bowel disease (IBD) in genetically susceptible hosts, yet limited information exists about oral microbes in IBD. We hypothesize that the microbiology of the oral cavity may differ in patients with IBD.
View Article and Find Full Text PDFCopper-catalyzed coupling of oxime acetates with aldehydes offers a new strategy for the synthesis of highly substituted pyridines. This novel method tolerates a wide range of functionality and allows for rapid elaboration of the oxime acetates into a variety of substituted pyridines.
View Article and Find Full Text PDFWhile human leukocyte antigen B57 (HLA-B57) is associated with the spontaneous clearance of hepatitis C virus (HCV), the mechanisms behind this control remain unclear. Immunodominant CD8(+) T cell responses against the B57-restricted epitopes comprised of residues 2629 to 2637 of nonstructural protein 5B (NS5B(2629-2637)) (KSKKTPMGF) and E2(541-549) (NTRPPLGNW) were recently shown to be crucial in the control of HCV infection. Here, we investigated whether the selection of deleterious cytotoxic T lymphocyte (CTL) escape mutations in the NS5B KSKKTPMGF epitope might impair viral replication and contribute to the B57-mediated control of HCV.
View Article and Find Full Text PDFCyclin D1 is a component of the core cell cycle machinery. Abnormally high levels of cyclin D1 are detected in many human cancer types. To elucidate the molecular functions of cyclin D1 in human cancers, we performed a proteomic screen for cyclin D1 protein partners in several types of human tumours.
View Article and Find Full Text PDFAn unprecedented ukv topological framework, based on a rod-like cadium-carboxylate chain, exhibits high CO(2) sorption heat and sorption selectivities of CO(2) over N(2) and CH(4).
View Article and Find Full Text PDFCuI-catalyzed reductive acylation of ketoximes for preparation of enamides was reported. A broad scope of enamides was obtained in high yields with NaHSO(3) used as the terminal reductant.
View Article and Find Full Text PDFUnder different situations, solvothermal reactions of 3,5-diethyl-4-(4-pyridyl)-pyrazole (HL) with CuX or CuX(2) (X = Cl, Br, I, and SCN) afforded five copper(I) coordination polymers, {CuX[CuL](3)·solvent}(n) (X = Cl, 1; Br, 2; I, 3; X = SCN and solvent = MeCN, 4) and {Cu(2)I(2)[CuL](3)}(n) (5). X-ray diffraction analyses show that all the complexes have trinuclear [CuL](3) (referred as Cu(3)) secondary building units featuring planar nine-membered Cu(3)N(6) metallocycles with three peripheral pyridyl groups as connectors, which are further linked by CuX or Cu(2)X(2) motifs to generate single- or double-strand chains. Interestingly, the Cu(I) atoms within the Cu(3) units in 1-5 behave as coordinatively unsaturated π-acid centers to contact soft halide/pseudohalide X atoms of CuX and Cu(2)X(2) motifs, which lead to novel sandwich substructures of [(Cu(3))(Cu(2)X(2))(Cu(3))] (X = Br, I, and SCN) in 2-4.
View Article and Find Full Text PDFSix new coordination polymers, namely {[Zn(btec)(0.5)(btmb)]·2H(2)O}(n) (1), {[Co(btec)(0.5)(btmb)(H(2)O)]·3H(2)O}(n) (2), {[Cu(btec)(0.
View Article and Find Full Text PDFTwo unique six-connected self-penetrating coordination polymers with a new (4(4).6(10).8) network topology, derived from the cross-linking of two 6(6)-dia subnets, were constructed from Ni(II) or Co(II) and two types of V-shaped tectons.
View Article and Find Full Text PDFA fascinating 3D entangled metal-organic framework, namely, {[Co(2)(bcp)(2)]·3H(2)O}(n) (1), was obtained through the solvothermal generation of flexible and long dicarboxylate (bcp) and metal salt. The crystal structure contains a 1D metal chain with bcp ligands wrapped around it in a wavy line and features an unusual entangled topological net. Furthermore, the magnetic behavior of 1 was also studied and indicated the existence of ferromagnetic interaction and long-range ordering character.
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