genomes recovered from Baltic Sea samples in Denmark.

We report the genomic characteristics of the human pathogen isolated from seawater and green algae in the Baltic Sea. Initially misidentified as through culture and MALDI-TOF, whole-genome sequencing (WGS) confirmed them as , highlighting the importance of WGS analysis in accurate classification of emerging pathogens.

Genomic Insights into : Molecular Epidemiology and Antimicrobial Resistance in Striped Catfish () Aquaculture in Vietnam.

is responsible for causing bacillary necrosis (BNP) in striped catfish () in Vietnam. This study offers a comprehensive genomic characterization of to enhance understanding of the molecular epidemiology, virulence, and antimicrobial resistance. isolates were collected from diseased striped catfish in the Mekong Delta.

Spontaneous fermentation mitigates the frequency of genes encoding antimicrobial resistance spreading from the phyllosphere reservoir to the diet.

The phyllosphere microbiome of vegetable products constitutes an important reservoir for multidrug resistant bacteria and Antibiotic Resistance Genes (ARG). Vegetable products including fermented products such as Paocai therefore may serve as a shuttle for extrinsic microorganisms with ARGs into the gut of consumers. Here we study the effect of fermentation on Paocai ARG dissemination by metagenomic analysis.

Nanopore sequencing for identification and characterization of antimicrobial-resistant and spp. from tilapia and shrimp sold at wet markets in Dhaka, Bangladesh.

Wet markets in low-and middle-income countries are often reported to have inadequate sanitation resulting in fecal contamination of sold produce. Consumption of contaminated wet market-sourced foods has been linked to individual illness and disease outbreaks. This pilot study, conducted in two major wet markets in Dhaka city, Bangladesh during a 4-month period in 2021 aimed to assess the occurrence and characteristics of and non-typhoidal spp.

Comparative genomic analysis of and from diseased striped catfish fingerlings cultured in Vietnam.

Introduction: Motile septicemia (MAS) is a burden for striped catfish () farmers in Vietnam. MAS can be caused by several species of but is seen as the leading cause of MAS in aquaculture, but recent reports suggest that is also causing MAS.

Methods: Here we investigated the bacterial etiology of MAS and compared the genomic features of and .

Distribution and ecology of the generalist lactic acid bacterium Carnobacterium maltaromaticum in different freshwater habitats: Metabolic and antagonistic abilities.

We explored the distribution, metabolic and antagonistic activities of Carnobacterium maltaromaticum, isolated from freshwater locations in Denmark during winter or early spring. This species was widely distributed in such habitats although it was relatively rare in low pH locations. Isolates possessed a diverse metabolism, potentially enabling functional capacities independent of habitat.

Whole strains vs MGEs in short and longterm transmission of ESBL genes between healthcare and community settings in Uganda.

Multidrug-resistant ESBL-producing Escherichia coli are a leading cause of infections in hospital and community settings. Based on samples from two hospitals in Uganda and households of inpatients we tested the hypothesis that ESBL E. coli and/or their resistance determinants could spread within the healthcare and community settings through discharged patients that were still colonized.

Salmonella Heidelberg and Salmonella Minnesota in Brazilian broilers: Genomic characterization of third-generation cephalosporin and fluoroquinolone-resistant strains.

Salmonella serovars Heidelberg and Minnesota encoding antimicrobial resistance to third-generation cephalosporins and fluoroquinolones are often detected in poultry/poultry meat. We analysed the genomes of 10 Salmonella Heidelberg (SH) and 4 Salmonella Minnesota (SM) from faecal isolates of Brazilian poultry. These featured virulent and multidrug-resistant characteristics, with AmpC beta-lactamase (bla ) predominance (9/14), for all SM (4/4) and some SH (3/10) located on IncC plasmid replicons.

Characterization of and other bacteria isolated from condemned broilers at a Danish abattoir.

Meat inspection is important to ensure food safety and protect public health. Visual inspection of slaughtered carcasses for pathological changes should be supported by bacteriological analysis to determine whether the entire carcass or parts of it should be condemned. The aim of this study was to determine the bacterial species present in different sample types from condemned broiler carcasses.

Fecal Shedding of Multidrug Resistant Isolates in Dogs Fed with Raw Meat-Based Diets in Brazil.

The practice of feeding dogs raw meat-based diets (RMBDs) is growing in several countries, and the risks associated with the ingestion of pathogenic and antimicrobial-resistant in dogs fed these diets are largely unknown. We characterized strains isolated from dogs fed either an RMBD or a conventional dry feed, according to the phylogroup, virulence genes, and antimicrobial susceptibility profiles of the bacteria. Two hundred and sixteen strains were isolated.

Salmonella Salamae and S. Waycross isolated from Nile perch in Lake Victoria show limited human pathogenic potential.

Non-enterica subspecies of Salmonella enterica are rarely associated with human infections. Paradoxically, food safety legislations consider the entire genus Salmonella as pathogenic to humans. Globally, large amounts of seafoods are rejected and wasted due to findings of Salmonella.

Genomic and Phenotypic Insights for Toxigenic Clinical Vibrio cholerae O141.

Vibrio cholerae remains a major public health threat worldwide, causing millions of cholera cases each year. Although much is known about the evolution and pathogenicity of the O1/O139 serogroups of V. cholerae, information is lacking on the molecular epidemiology of non‒O1/O139 strains isolated from patients who have diarrheal illnesses.

Genetic Comparison of ESBL-Producing from Workers and Pigs at Vietnamese Pig Farms.

We analyzed and compared genomes of Extended Spectrum Beta-Lactamase (ESBL)-producing from pigs and pig farm workers at 116 farms in Vietnam. Analyses revealed the presence of , , , , , , , , and in both hosts. Most strains from pigs contained quinolones () and colistin resistance genes (-1 and -3).

ESBL and C β-Lactamase Encoding Genes in From Pig and Pig Farm Workers in Vietnam and Their Association With Mobile Genetic Elements.

Animals are considered important sources of ESBL/AmpC-producing bacteria in humans. We analyzed indications of transfer of ESBL/AmpC genes between pigs and pig farmers in Vietnam by analyzing whole genome sequences of 114 ESBL/AmpC-producing isolated from the two hosts, and performed conjugation experiments and plasmid profiling to confirm that such transfer could have happened. ESBL-encoding genes detected in pigs and pig farmers included , , , , , , , and , and AmpC β-lactamases included , , and .

Molecular Characteristics and Zoonotic Potential of Weltevreden From Cultured Shrimp and Tilapia in Vietnam and China.

Weltevreden is increasingly reported from aquatic environments, seafood, and patients in several Southeast Asian countries. Using genome-wide analysis, we characterized . Weltevreden isolated from cultured shrimp and tilapia from Vietnam and China to study their genetic characteristics and relatedness to clinical isolates of .

Prevalence and genomic characterization of Salmonella Weltevreden in commercial pig feed.

Viet Nam is the world's fifth largest producer of pork meat. Salmonella is frequently found at farm level; however, risk factors for Salmonella infection in pigs have not been thoroughly investigated in the production system. In the current study, 123 commercial feed samples were obtained from 103 small, medium and large-scale pig farms in Viet Nam and investigated for the presence of Salmonella in 25 g of feed using the ISO 6579:2002/Cor 1:2004 method.

Genome-Based Analysis of Extended-Spectrum β-Lactamase-Producing in the Aquatic Environment and Nile Perch () of Lake Victoria, Tanzania.

Extended-spectrum β-lactamase (ESBL)-producing bacteria constitute an emerging global health issue with food products being vehicles of transmission and the aquatic environments serving as potential reservoirs. This study aimed to characterize ESBL-producing in Nile perch and water from Lake Victoria in Tanzania. A total of 180 samples of Nile perch and 60 water samples were screened for ESBL-producing on MacConkey agar supplemented with 2 μg/ml of cefotaxime and confirmed by and PCR.

Corrigendum: Surveillance and Genomics of Toxigenic O1 From Fish, Phytoplankton and Water in Lake Victoria, Tanzania.

[This corrects the article DOI: 10.3389/fmicb.2019.

Genomic insights into Vibrio cholerae O1 responsible for cholera epidemics in Tanzania between 1993 and 2017.

Background: Tanzania is one of seven countries with the highest disease burden caused by cholera in Africa. We studied the evolution of Vibrio cholerae O1 isolated in Tanzania during the past three decades.

Methodology/principal Findings: Genome-wide analysis was performed to characterize V.

Cholera hotspots and surveillance constraints contributing to recurrent epidemics in Tanzania.

Objective: We described the dynamics of cholera in Tanzania between 2007 and 2017 and assessed the weaknesses of the current surveillance system in providing necessary data in achieving the global roadmap to 2030 for cholera control.

Results: The Poisson-based spatial scan identified cholera hotspots in mainland Tanzania. A zero-inflated Poisson regression investigated the relationship between the incidence of cholera and available demographic, socio-economic and climatic exposure variables.

Tilapia () as a Putative Reservoir Host for Survival and Transmission of O1 Biotype El Tor in the Aquatic Environment.

Studies have reported the occurrence of in fish but little is known about the interaction between fish and toxigenic as opposed to phytoplankton, which are well-established aquatic reservoirs for . The present study determined the role of tilapia () as a reservoir host for survival and transmission of in aquatic environments. Three experiments were performed with one repetition each, where (∼2 g) kept in beakers were inoculated with four strains (5 × 10 cfu/mL).

Surveillance and Genomics of Toxigenic O1 From Fish, Phytoplankton and Water in Lake Victoria, Tanzania.

The occurrence of toxigenic O1 during a non- outbreak period in Lake Victoria was studied and genetic characteristics for environmental persistence and relatedness to pandemic strains were assessed. We analyzed 360 samples of carps, phytoplankton and water collected in 2017 during dry and rainy seasons in the Tanzanian basin of Lake Victoria. Samples were tested using PCR (W and A) with DNA extracted from bacterial isolates and samples enriched in alkaline peptone water.