Publications by authors named "Yaoli Shi"
Critical role of ROCK1 in AD pathogenesis via controlling lysosomal biogenesis and acidification.
Transl Neurodegener
November 2024
Article Synopsis
- Increased levels of ROCK1 in the brains of Alzheimer's disease (AD) patients correlate positively with lysosomal dysfunction and Aβ accumulation, suggesting its role in AD pathogenesis.
- The study demonstrates that knocking down ROCK1 enhances lysosomal function and promotes the clearance of Aβ by facilitating TFEB's nuclear distribution.
- Targeting ROCK1 may provide a potential therapeutic strategy to restore lysosomal homeostasis and mitigate cognitive decline in AD models.*
Article Synopsis
- Autophagy is a crucial process in cells that relates to aging and diseases, and real-time imaging of this process is important for identifying defects and developing treatments.
- A new fluorescent probe made from a thiacyanine dye called PTC was created to monitor autophagy in living cells, showing significant sensitivity to changes in viscosity and G-quadruplex structures.
- This probe enhances fluorescence detection by over 2000 times, providing a reliable and specific tool for autophagy research, with potential applications due to its high sensitivity, low cost, and ease of use.
Mitochondria are crucial powerhouses and central organelles for maintaining normal physiological activities in eukaryotic cells. The use of highly specific optical biosensors to monitor mitochondrial autophagy (mitophagy) is an important way for detecting mitochondrial abnormalities. Herein, we report a pH responsive G-quadruplex (G4) structure folded by the oligonucleotide named P24.
View Article and Find Full Text PDFPurpose: Despite the revealed role of immunological dysfunctions in the development and progression of Alzheimer's disease (AD) through animal and postmortem investigations, direct evidence regarding the impact of genetic factors on microglia response and amyloid-β (Aβ) deposition in AD individuals is lacking. This study aims to elucidate this mechanism by integrating transcriptomics and TSPO, Aβ PET imaging in clinical AD cohort.
Methods: We analyzed 85 patients with PET/MR imaging for microglial activation (TSPO, [F]DPA-714) and Aβ ([F]AV-45) within the prospective Alzheimer's Disease Immunization and Microbiota Initiative Study Cohort (ADIMIC).
Background: The gene encoding the E3 ubiquitin ligase substrate-binding adapter Speckle-type BTB/POZ protein (SPOP) is frequently mutated in prostate cancer (PCa) and endometrial cancer (EC); however, the molecular mechanisms underlying the contribution of SPOP mutations to tumorigenesis remain poorly understood.
Methods: BRAF harbors a potential SPOP-binding consensus motif (SBC) motif. Co-immunoprecipitation assays demonstrated that BRAF interacts with SPOP.
Background: The gene encoding the E3 ubiquitin ligase substrate-binding adaptor SPOP is frequently mutated in primary prostate cancer, but how SPOP mutations contribute to prostate cancer pathogenesis remains poorly understood. Stress granules (SG) assembly is an evolutionarily conserved strategy for survival of cells under stress, and often upregulated in human cancers. We investigated the role of SPOP mutations in aberrant activation of the SG in prostate cancer and explored the relevanve of the mechanism in therapy resistance.
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