Publications by authors named "Yaokun Liang"
The CRISPR/Cas9 systems have been developed as tools for genetic engineering and metabolic engineering in various organisms. In this review, various aspects of CRISPR/Cas9 in , from basic principles to practical applications, have been summarized. First, a comprehensive review has been conducted on the history of CRISPR/Cas9, successful cases of gene disruptions, and efficiencies of multiple DNA fragment insertions.
View Article and Find Full Text PDFArticle Synopsis
- Researchers focused on the β-xylosidase enzyme BH3683 to form microparticles with iron sulfate, discovering that biological molecules greatly influence their formation and enzymatic activity.
- The resulting FeSO-CE-MOF-BH3683 microparticles showed increased thermal stability, better tolerance to solvents and xylose, and maintained significant activity even after multiple uses, suggesting strong potential for applications in biocatalysis and related fields. *
Here a versatile fusion tag composed of His-tag, intein, and elastin-like polypeptide (ELP) tag was prepared for the first time to be fused with levansucrase SacB to construct a recombinant His-ELP-intein-SacB (HEIS) protein to realize nonchromatographic purification of SacB. The efficient biomimetic mineralization of CaHPO and HEIS-based hybrid-hydrangea (CaHPO-HEIS-HH) with good reusability, excellent storage stability and 254.3% improved relative levan yield was prepared with the biomimetic mineralization method.
View Article and Find Full Text PDF