Machine-Learning Mental-Fatigue-Measuring μm-Thick Elastic Epidermal Electronics (MMMEEE).

Electrophysiological (EP) signals are key biomarkers for monitoring mental fatigue (MF) and general health, but state-of-the-art wearable EP-based MF monitoring systems are bulky and require user-specific, labeled data. Ultrathin epidermal electrodes with high performance are ideal for constructing imperceptive EP sensing systems; however, the lack of a simple and scalable fabrication delays their application in MF recognition. Here, we report a facile, scalable printing-welding-transferring strategy (PWT) for printing μm-thickness micropatterned silver nanowires (AgNWs)/sticky polydimethylsiloxane, welding the AgNWs via plasmonic effect, and transferring the electrode to skin as tattoos.

Polymorphisms of PHF11 and DPP10 are associated with asthma and related traits in a Chinese population.

Background: Initial studies by positional cloning have identified the genes encoding the plant homeodomain zinc finger protein 11 (PHF11) and dipeptidyl-peptidase 10 (DPP10) as asthma susceptibility genes. The variants in the two genes have been associated with asthma in several populations of European or Latin American origin.

Objective: The aim of this study was to assess the common PHF11 and DPP10 polymorphisms for associations to asthma and asthma-related traits in a Han Chinese population.

[T-bet gene modified dendritic cells abrogate and reverse the airway inflammation in allergic asthma: experiment with mice].

Objective: To explore the feasibility of the therapeutic strategy to use T-bet gene modified dendritic cells (DCs) to reverse the course of asthma.

Methods: (1) Mature DCs were derived from mononuclear cells obtained from femur of BALB/c mouse and divided into 3 groups, T-bet group transfected with recombinant adenovirus Ad-T-bet containing T-bet gene, LacZ group transfected with recombinant adenovirus Ad-LacZ containing LacZ gene, and control group. Seven days later ELISA was used to detect the interferon (IFN)-gamma level in the culture fluid.

[Vasoactive intestinal peptide expression and its clinical significance in gastric adenocarcinoma].

Objective: To investigate the expression of vasoactive intestinal peptide (VIP) in gastric adenocarcinoma, and to evaluate the correlation of VIP level with clinical pathologic parameters.

Methods: The level of VIP in sera from gastric adenocarcinoma patients and healthy people was investigated by ELISA. Moreover, the differential gene expression between gastric adenocarcinoma, gastric dysplasia, and the corresponding normal gastric mucosa were determined by RT-PCR.

[Continuous inhalation of allergen induces immunotolerance in a mouse asthma model].

Objective: To investigate the mechanism of immunotolerance caused by allergen immunotherapy in allergen-induced asthmatic airway inflammation.

Methods: Sixty ovalbumin (OVA)-sensitized BALB/c mice were randomly divided into two groups, 50 in the experimental group and 10 in the control group. The mice in the experimental group were treated with 3 injections of ovalbumin intraperitoneally (1 mg for each separated for two weeks) and challenged by ovalbumin inhalation 1 h/day for 10 successive days.

[The roles of dendritic cells in antigen presentation and the pathogenesis of asthma].

Objective: To investigate the roles of dendritic cells (DCs) in the pathogenesis of asthma.

Methods: (1) DCs were derived from peripheral blood monocytes of asthmatics with acute exacerbation (group A, n = 20), patients at remission (group B, n = 15) and healthy volunteers (group C, n = 10), and cultured using media supplemented with granulocyte-macrophage colony stimulation factors (GM-CSF), interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-alpha). The antigen-uptaking function of the monocyte-derived DCs was evaluated by phagocytosis of fluorescence labeled ovalbumin (OVA), and their antigen-presenting function was determined by expression of membrane markers (MHC-II) and co-stimulatory molecules (CD(80) and CD(86)).

Beta2-adrenergic receptor gene polymorphism in Chinese Northern asthmatics.

Objective: To investigate whether the polymorphisms of beta2-adrenergic receptor (beta2-AR) at position 16, 27, 164 are associated with asthma in Northern Chinese subjects.

Methods: Genomic DNA was collected from unrelated Northern Chinese population of Han ethnicity, including 125 unrelated asthmatic individuals and 96 healthy controls. Beta2-AR genes at position 16, 27, 164 were amplified by using restriction fragment length polymorphism (RFLP) and allelic specific polymerase chain reaction methods.

[The evolvement of Th1/Th2 imbalance accommodates to the progress of airway inflammation in asthmatic subjects and rat model].

Objective: To observe the production of IFN-gamma and IL-4 released in peripheral T lymphocytes in asthmatic patients and rat models and to clarify the dynamic changes of proliferation and differentiation of T lymphocytes during the progress of airway inflammation.

Methods: Twenty patients with moderate to severe acute exacerbation and 15 patients with remission of asthma were included in this study. Ten normal volunteers were also enrolled as control group.

[The evolvement of Th1/Th2 imbalance in the process of airway inflammation in a rat asthmatic model].

Objective: T lymphocytes play an important role in the development of asthmatic airway inflammation. By observing the production of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) released by peripheral T lymphocytes in asthmatic rat models, this study was designed to clarify the changes of T lymphocytes during the course of airway inflammation. The influence of dexamethasone pre-treatment on the production of IFN-gamma and IL-4 by T lymphocytes was also investigated in the study.

[A study on the active efflux mechanism in Staphylococcus aureus resistant to quinolone].

Objective: To explore the effect, the characteristic, and potential approaches to the active efflux mechanism in straphylococcus aureus (S. a) resistant to quinolones.

Methods: S.

[Association of polymorphism of human beta 2-adrenergic receptor gene and bronchial asthma].

Objective: To investigate whether beta 2-adrenergic receptor gene (beta 2AR) polymorphism at position 16, 27, 164 is in association with asthma susceptibility or asthmatic phenotype (including nocturnal asthma, serum IgE level, bronchial responsiveness, the status of asthmatics).

Methods: By using PCR-RFLP and allelic-specific PCR (ASP), the polymorphism of beta 2AR gene at position 16, 27, 164 in 125 Han origin asthmatics and 96 normal healthy controls with the same ethnic nearby Beijing region were genotyped. All patients had their serum total IgE (TIgE) measured by RAST, pulmonary ventilatory function assessed by FEV1% and FEV1/FVC, bronchial responsiveness challenged by methacholine (if FEV1% > 70%), and brocho-reversibity by inhaling beta 2-agonist.

Association between HLA-DQA1, -DQB1 gene polymorphisms and susceptibility to asthma in northern Chinese subjects.

Objective: Human leukocyte antigen (HLA) class II genes, especially HLA-DQ genes, which are highly polymorphic, have been thought to be candidate loci for the etiology of asthma, and shown to be involved in antigenic presentation. This study was conducted to investigate whether susceptibility or resistance to asthma is associated with HLA-DQA1 and DQB1 genes polymorphism.

Methods: Venous blood samples were collected from northern Chinese population with Han ethnic.

Effect of supplementing a high-fat, low-carbohydrate enteral formula in COPD patients.

Objective: One of the goals in treating patients with chronic obstructive pulmonary disease (COPD) who suffer from hypoxemia, hypercapnia, and malnutrition is to correct the malnutrition without increasing the respiratory quotient and minimize the production of carbon dioxide. This 3-wk study evaluated the efficacy of feeding a high-fat, low-carbohydrate (CHO) nutritional supplement as opposed to a high-carbohydrate diet in COPD patients on parameters of pulmonary function.S METHODS: Sixty COPD patients with low body weight (<90% ideal body weight) were randomized to the control group, which received dietary counseling for a high-CHO diet (15% protein, 20% to 30% fat, and 60% to 70% CHO), or the experimental group, which received two to three cans (237 mL/can) of a high-fat, low-CHO oral supplement (16.

[Epidemiological survey of the incidence of bronchial asthma in occupational populations in Beijing area].

Objective: To investigate the correlation of asthma incidence with environmental pollution.

Methods: We performed stratified-cluster-disproportional-random-sampling survey in the occupational populations from 10 professional systems by using an uniform protocol, questionnaire and procedure in this multicenter study. Original data were processed and statistically analyzed with SPSS 8.

[Primary pulmonary lymphoma: analysis of cases and review of the literature].

Objective: To study the clinical characteristics, diagnosis and treatment of primary pulmonary lymphoma.

Methods: Six cases of primary pulmonary lymphoma diagnosed from 1989 to 2000 were retrospectively analyzed, and the literature was reviewed with regard to its clinical manifestations, X-ray features, bronchoscopic findings, diagnosis and treatment.

Results: Primary pulmonary lymphoma was a rare form of lymphoma.

[Relationship between HLA-DQA1, -DQB1 genes polymorphism and susceptilibity to bronchial asthma among Northern Hans].

Objective: To investigate the relationship between human leukocyte antigen (HLA)- DQA1, -DQB1 genes polymorphism and susceptibility to bronchial asthma among the Hans in the northern China.

Method: Sequence-specific primer polymerase chain reaction was used to gene frequencies of HLA-DQA1 and HLA-DQB1 genes among 125 unrelated asthmatics, 12 of which being probands of asthmatic pedigrees, and 96 healthy controls, all of Han nationality and living in Beijing or nearby areas for a long time. Radioimmunosorbent test was used to examine the serum IgE and TigE.

[CT pulmonary functional imaging in emphysema: a correlative study of CT technique and pulmonary function test].

Objective: To compare the effectiveness of high resolution CT, CT pulmonary functional imaging, and pulmonary function test (PFT) in the quantitative evaluation of emphysema and obstructive pulmonary disease.

Methods: The study included 37 patients with obstructive ventilatory defect and 20 normal controls. CT and pulmonary function test were performed in all subjects in one week.