Discovery of Bioactive Terpenes Derived from a Polar Fungus.

Polar fungi play a vital role as prolific sources of unique chemical structures and diverse bioactive compounds. Eutypella sp. D-1 is a fungus isolated from the Arctic, and six compounds were extracted from the fermentation broth.

Macrolactin XY, a Macrolactin Antibiotic from Marine-Derived sp. 18.

Two new compounds, macrolactin XY () and (5, 9, 10)-5-(hydroxymethyl)-1,3,7-decatriene-9,10-diol (), together with nine known compounds (-) were isolated from the marine sp. 18 by the OSMAC strategy. These compounds were evaluated for antibacterial activity against six tested microorganisms.

Genome Mining Leads to Diverse Sesquiterpenes with Anti-inflammatory Activity from an Arctic-Derived Fungus.

With the advancement of bioinformatics, the integration of genome mining with efficient separation technology enables the discovery of a greater number of novel bioactive compounds. The deletion of the key gene responsible for triterpene cyclase biosynthesis in the polar strain sp. D-1 instigated metabolic shunting, resulting in the activation of dormant genes and the subsequent production of detectable, new compounds.

Pimarane-Type Diterpenes with Anti-Inflammatory Activity from Arctic-Derived Fungus sp. D-1.

The Arctic-derived fungus sp. D-1 can produce numerous secondary metabolites, and some compounds exhibit excellent biological activity. Seven pimarane-type diterpenes, including three new compounds eutypellenone F (), libertellenone Y (), and libertellenone Z (), and four known compounds (-), were isolated from fermentation broth of sp.

Saturated and Polyunsaturated Fatty Acids Production by PKU#Mn4 on Hydrolysate.

Thraustochytrids are unicellular marine heterotrophic protists, which have recently shown a promising ability to produce omega-3 fatty acids from lignocellulosic hydrolysates and wastewaters. Here we studied the biorefinery potential of the dilute acid-pretreated marine macroalgae () in comparison with glucose via fermentation using a previously isolated thraustochytrid strain ( PKU#Mn4). The total reducing sugars in the hydrolysate accounted for 43.

Application of Gene Knockout and Heterologous Expression Strategy in Fungal Secondary Metabolites Biosynthesis.

The in-depth study of fungal secondary metabolites (SMs) over the past few years has led to the discovery of a vast number of novel fungal SMs, some of which possess good biological activity. However, because of the limitations of the traditional natural product mining methods, the discovery of new SMs has become increasingly difficult. In recent years, with the rapid development of gene sequencing technology and bioinformatics, new breakthroughs have been made in the study of fungal SMs, and more fungal biosynthetic gene clusters of SMs have been discovered, which shows that the fungi still have a considerable potential to produce SMs.

Optimization of Protoplast Preparation and Establishment of Genetic Transformation System of an Arctic-Derived Fungus sp.

Arctic-derived fungus sp. D-1 has attracted wide attention due to its huge ability to synthesize secondary metabolites. However, current studies only focus on stimulating its production of new secondary metabolites by OSMAC strategies, and the relationship between secondary metabolites and biosynthetic gene clusters (BGCs) has not been explored.