METTL3/YTHDC1 mediates up-regulation of lncRNA OGRU in an m6A-dependent manner involving in oxidative stress and inflammation of HG-induced Müller cells.

Background: Diabetic retinopathy (DR) is a common complication of diabetes, which may cause visual disturbance and even loss of sight. Oxidative stress and inflammation are two crucial pathological factors of DR; however, their specific regulatory mechanisms in DR remain obscure.

Methods: DR models were established in streptozotocin-challenged rats and high glucose (HG)-stimulated Müller cells.

The Effect of TiC-TiB Dual-Phase Nanoparticles on the Microstructure and Mechanical Properties of Cast Ni-Fe-Based Superalloys.

TiC-TiB dual-phase nanoparticles were added into a Ni-Fe-based cast superalloy and their effects on the microstructure and mechanical properties were compared to those of a Ni-Fe-based superalloy with the addition of TiC nanoparticles. The addition of TiC nanoparticles led to the precipitation of a higher volume fraction of carbides. Compared to the addition of TiC, the addition of TiC-TiB nanoparticles not only led to the precipitation of carbides but also promoted the formation of flaky borides and a reduction in the precipitation of the Laves phase.

Müller Cells Harboring Exosomal lncRNA OGRU Modulate Microglia Polarization in Diabetic Retinopathy by Serving as miRNA Sponges.

Diabetic retinopathy (DR) is one of the most common complications of diabetes worldwide and is associated with visual loss and blindness. However, effective treatments for both early- and late-stage DR remain lacking. A streptozotocin-induced diabetic mouse model and high glucose (HG)-treated Müller cell model were established.

A red-emission fluorescence probe based on 1,4-addition reaction mechanism for the detection of biothiols in vitro and in vivo.

In this work, a new fluorescence probe (DC) with a donor-π-acceptor (D-π-A) structure was designed and synthesized for the detection of three kinds of biothiols (Cys, Hcy and GSH) in live cells and organisms. DC displayed an intense red-emission centered at 625 nm. In the presence of biothiols, the nucleophilic addition reaction between the C=C double bond of DC and the sulfhydryl group (-SH) of biothiols occurred, resulting in obvious fluorescence quenching responses.

A phenothiazine-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in food, live cells and animals.

In this work, a phenothiazine-based fluorescent probe (PR) has been developed for the selective detection of hydrogen sulfide (HS) in biosystems and monitoring HS produced in the food spoilage process. The nucleophilic attack of HS on the CC double bond of PR a Michael addition interdicted the ICT process to trigger 34-fold enhancement of the fluorescence emission. PR featured high selectivity and sensitivity (1.