Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3.

Osteoarthritis (OA) affects approximately 12% of the aging Western population. The sirtuin/forkhead box O (SIRT/FOXO) signaling pathway plays essential roles in various biological processes. Despite it has been demonstrated that ubiquitin-specific protease 3 (USP3) inhibits chondrocyte apoptosis induced by interleukin (IL)-1β, the role of USP3/SIRT3/FOXO3 in the senescence of chondrocytes in OA is unclear.

MicroRNA-221 regulates osteosarcoma cell proliferation, apoptosis, migration, and invasion by targeting CDKN1B/p27.

MicroRNAs (miRNAs, miR) are of critical importance in growth and metastasis of cancer cells; however, the underlying functions of miRNAs in osteosarcoma (OS) remain largely unknown. This study was aimed to elucidate the role of miR-221 in regulating the biological behavior of OS cells. The proliferation ability was examined by cell counting kit-8 (CCK-8) and cell cycle assay.

MicroRNA-34a promotes cell cycle arrest and apoptosis and suppresses cell adhesion by targeting DUSP1 in osteosarcoma.

MicroRNAs are often deregulated in most cancer types and have important functions in carcinogenesis and cancer progression. Here, we studied the function of microRNA-34 (miR-34a) in osteosarcoma MG63 and U-2OS cells by expressed with pre-miR-34a, anti-miR-34a and corresponding negative controls, respectively. Cells proliferation, cell cycle and apoptosis was measured by MTT and flow cytometry assay.

Treatment of femoral neck fracture with percutaneous compression plate: preliminary results in 74 patients.

Objective: The purpose of this study was to present the surgical technique and clinical results of percutaneous compression plate (PCCP) for the treatment of femoral neck fractures.

Methods: Between December 2010 and April 2013, 74 consecutive patients with 74 femoral neck fractures were treated by closed reduction and PCCP implants in our university hospital. Their mean age was 51.

Cannabinoid receptor-2 selective antagonist negatively regulates receptor activator of nuclear factor kappa B ligand mediated osteoclastogenesis.

Background: The cannabinoid receptor-2 (CB2) is important for bone remodeling. In this study, we investigated the effects of CB2 selective antagonist (AM630) on receptor activator of nuclear factor kappa B (RANK) ligand (RANKL) induced osteoclast differentiation and the underlying signaling pathway using a monocyte-macrophage cell line-RAW264.7.

ACDF with the PCB cage-plate system versus laminoplasty for multilevel cervical spondylotic myelopathy.

Study Design: A nonrandomized controlled trial.

Objective: To compare the clinical outcomes, radiographic changes, and complications of patients with multilevel cervical spondylotic myelopathy who underwent ACDF with the plate cage benezech (PCB) implant system and laminoplasty.

Summary Of Background Data: Using anterior or posterior surgery for multilevel cervical spondylotic myelopathy continues to be the subject of considerable debate.

Kyphoplasty does not maintain all restored height postoperatively: a prospective, comparative study.

From January to December 2008, balloon kyphoplasty was performed on 45 consecutive female patients with primary single-segment vertebral compression fractures as an inpatient procedure. All of the treated vertebral bodies were located within the thora-columbar region (T11-L2). Demographic data such as age, body mass index, fracture age, hospital stay, lumbar spine bone mineral density, and amount of bone cement injected per vertebrae were recorded.

[Long-term outcomes of the revision open lumbar discectomy by fenestration: a follow-up study of more than 10 years].

Objective: To evaluate the long-term clinical results and the factors that influences the outcomes of the revision open lumbar discectomy by fenestration.

Methods: Fifty-one patients, who underwent the second open discectomy by fenestration from January 1 1988 to December 31 1994, were followed for an average of 146.8 months (range, 120 to 203 months).