Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Imported From Africa to China.

surface-related antigen (SRA) is located on the surfaces of gametocyte and merozoite and has the structural and functional characteristics of potential targets for multistage vaccine development. However, little information is available regarding the genetic polymorphism of . To determine the extent of genetic variation about by characterizing the sequence, 74 samples were collected from migrant workers who returned to China from 12 countries of Africa between 2015 and 2019.

Classification of induced malaria case in an elimination setting: investigation of transfusion-transmitted malaria cases.

Background: Since the National Malaria Elimination Action Plan was launched in China in 2010, local malaria transmission has decreased rapidly. Zero indigenous cases were reported since 2017. However, after 2010, the proportion of imported cases in China increased from 45.

[Progress in researches on molecular markers of non- drug resistance].

With the long-term and widespread application of antimalarial drugs, has gradually produced resistance to antimalarial drugs. At present, there are more researches on the molecular markers of drug resistance, while less attention has been paid to the molecular markers of non- drug resistance. In order to provide the reference for rational drug use in clinical treatment and reference for molecular monitoring of antimalarial drug sensitivity of non-, this paper reviews the researches on the common molecular markers related to non drug resistance.

[Construction and application of malaria biobank].

Objective: To establish a systemized malaria biobank with well-rounded epidemiologic data and a computer-aid management system, thus to provide qualified sources for malaria elimination and human malaria research.

Methods: The malaria biobank was based on the platform of Jiangsu Institute of Parasitic Diseases. The blood smear samples, dried blood samples, whole blood samples and parasite strains isolated from patients (from both local and imported cases) were collected since 2011 according to a standardized operational procedure.

[Progress of malaria elimination of Jiangsu Province, China].

There were vivax malaria, falciparum malaria, and malariae malaria epidemic in Jiangsu Province, and vivax malaria was the dominant disease. and were malaria-transmitted vectors. Since the founding of People's Republic of China, there have been two major malaria outbreaks in Jiangsu Province, when the highest annual malaria incidence reached up to 24.

[Survey of schistosomiasis KAP and influencing factors of behaviors among residents in Jiangsu Province].

Objective: To understand the schistosomiasis control knowledge, attitude, and practice (KAP), and influencing factors of behaviors among residents in Jiangsu Province, so as to provide the evidence for making effective health education and health promotion models.

Methods: The probability proportionate to size sampling (PPS) and multi-stage sampling methods were adopted to sample the research objects. A questionnaire survey of schistosomiasis control KAP was conducted in the residents of 16 to 69 years old in schistosomiasis endemic areas of Jiangsu Province, and the results were statistically analyzed.

[Analysis of channels of going abroad of imported malaria patients in Jiangsu Province, China].

Objective: To analyze the channels of going abroad of imported malaria patients in Jiangsu Province, so as to provide the evidence for the control of imported malaria and health intervention for high-risk population.

Methods: The data of imported malaria cases in Jiangsu Province in 2015 were collected and the channels of going abroad were analyzed. The Quantum GIS 1.

Shrinking the malaria map in China: measuring the progress of the National Malaria Elimination Programme.

Background: Remarkable progress has been made towards the elimination of malaria in China since the National Malaria Elimination Programme (NMEP) was launched in 2010. The incidence of locally-acquired malaria cases has declined rapidly and endemic areas have also dramatically shrunk. In total, 3 078 malaria cases were reported in 2014, but only 56 cases were indigenous.

[Establishment of malaria early warning system in Jiangsu Province VII Eliminating malaria surveillance sentinels].

Objective: To understand the epidemic situation and influencing factors of malaria in Jiangsu Province and grasp the regularity and trend of the epidemic.

Methods: According to the provincial malaria transmission situation and characteristics, Sihong County of Suqian City, which had more original cases, and Baoying County of Yangzhou City, which had more import cases, were chosen as the malaria surveillance sentinels at the state level; six counties, Yixing County of Wuxi City, Suining County of Xuzhou City, Wujin District of Changzhou City, Haian county of Nantong City, Ganyu County of Lianyugang City, and Xuyi county of Huaian City were chosen as the malaria surveillance sentinels at the provincial level. The data of basic status, blood tests of feverish patients, case studies of malaria patients, investigations and disposals of epidemic focuses and others were collected and analyzed.

[Epidemiological analysis of malaria prevalence in Jiangsu Province in 2014].

Objective: To understand the malaria epidemic situation and characteristics in Jiangsu Province in 2014, so as to provide the evidence for formulating and adjusting appropriate strategies and measures for malaria elimination in this province.

Methods: The reported malaria cases from the Internet Reporting System and the epidemiological data of malaria in Jiangsu Province were collected and analyzed.

Results: A total of 355 malaria cases were reported in Jiangsu Province in 2014, which was increased by 4.

[Establishment of malaria early warning system in Jiangsu Province VI Regular surveillance of eliminating malaria and its effect].

Objective: To analyze the regular surveillance and evaluate its effect during the elimination stage of malaria in Jiangsu Province from 2012 to 2014 and discuss the best way for surveillance and management, so as to provide the evidence for formulating and adjusting the strategy and methods for malaria elimination.

Method: The data of network malaria reports, blood tests of feverish patients, epidemiology investigation forms of case study of malaria patients in the report system, and investigation and disposition forms of epidemic regions were collected and analyzed statistically.

Results: From 2012 to 2014, there were 894 malaria cases in total.

[Establishment of early warning system of malaria in Jiangsu Province V Es- tablishment of prevention and control system of imported falciparum malaria].

Objective: To establish a system of the prevention and control of imported falciparum malaria in Jiangsu Prov- ince and provide the new scientific basis for the prevention and control of imported falciparum malaria.

Methods: The data- bases for overseas labor companies and labors in Jiangsu Province were built and the health education was conducted to the overseas labors. The "1-3-7" elimination strategy was established.

[Analysis of epidemic situation of malaria in Jiangsu Province from 2005 to 2014].

Objective: To understand the epidemic situation and epidemiological characteristics of malaria in Jiangsu Province from 2005 to 2014, so as to provide the evidence for formulating and adjusting the strategies and measures on malaria elimination in this province.

Method: The epidemiological data of malaria in Jiangsu province from 2005 to 2014 were collected, and the prevalence situation, infection sources as well as the temporal, regional and population distribution of the cases were analyzed.

Result: A total of 5,069 malaria cases were reported in Jiangsu Province form 2005 to 2014, there were 3,422 cases (67.

[Establishment of malaria early warning system in Jiangsu Province IV Implementation of key measures to eliminate malaria in Jiangsu Province in 2013].

Objective: To promote the malaria control process in the malaria elimination stage in Jiangsu Province.

Methods: The data from the network reporting system of medical institutions, the disease prevention and control institutions at all levels, and the special reports system of parasitic diseases were collected and analyzed statistically.

Results: There were 341 malaria cases directly reported from the Jiangsu provincial network in 2013.

[Epidemiological analysis of malaria prevalence in Jiangsu Province in 2013].

Objective: To understand the malaria epidemic situation and characteristics in Jiangsu Province in 2013, so as to provide the evidence for formulating and adjusting effective malaria elimination strategies and measures.

Methods: The reported malaria cases from the Internet Reporting System and the epidemiological data of malaria in Jiangsu Province were collected and analyzed.

Results: A total of 341 malaria cases were reported in Jiangsu Province in 2013 with the incidence of 0.

[Surveillance of malaria in provincial surveillance sites in Jiangsu Province, 2013].

Objective: To understand the epidemic situation and influencing factors of malaria in Jiangsu Province and grasp its epidemic regularity and trend.

Methods: According to the malaria prevalence in Jiangsu Province, 6 counties (city, district) including Yixing, Suining, Wujin, Hai'an, Ganyu and Xuyi were selected as provincial surveillance sites to survey malaria epidemic conditions. The basic information, blood test results of fever patients, case investigation, information of malaria patients, monitoring data of investigation and disposition of the malaria focus were collected and analyzed.

[Establishment of malaria early warning system in Jiangsu Province III effect of automatic early warning information system on the response of malaria elimination].

Objective: To evaluate the effect of automatic early warning information system on the response of malaria elimination in Jiangsu Province through the operation of the national automatic early warning system of infectious diseases.

Methods: The malaria early warning information was collected from the automatic early warning information subsystem in the national information system for diseases control and prevention. Malaria early warning signals were analyzed from September 1 to December 31, 2012.

[Establishment of malaria early warning system in Jiangsu Province II application of digital earth system in malaria epidemic management and surveillance].

Objectives: To explore a new mode of malaria elimination through the application of digital earth system in malaria epidemic management and surveillance.

Methods: While we investigated the malaria cases and deal with the epidemic areas in Jiangsu Province in 2011, we used JISIBAO UniStrong G330 GIS data acquisition unit (GPS) to collect the latitude and longitude of the cases located, and then established a landmark library about early-warning areas and an image management system by using Google Earth Free 6.2 and its image processing software.

[Study on TaqMan-MGB real-time fluorescence quantitative PCR to detect gene mutation of kdr from Anopheles sinensis].

Objective: To establish a Real-time Fluorescence Quantitative PCR to detect the kdr gene mutation in Anopheles sinensis.

Methods: One pair of primers and three TaqMan-MGB probes were designed based on kdr gene and its L1014 locus mutations of A. sinensis.

[Analysis of overseas imported malaria situation and implication for control in Jiangsu Province, PR China].

Objective: To analyze the epidemiological characteristics of overseas imported malaria in Jiangsu Province and explore the strategies and priorities in prevention and control, so as to provide the evidence for improving the diagnosis, treatment and management of imported malaria.

Methods: The data of overseas imported malaria as well as the case epidemiological investigation in Jiangsu Province from July 18, 2011 to June 30, 2012 were collected and analyzed descriptively for the species composition, original countries, population distribution, regional distribution, onset time, diagnosis and treatment, channels to go abroad, and counterparts returned together with the patients.

Results: A total of 233 overseas imported malaria cases were reported, and 226 cases (97.

[Epidemiological analysis of malaria prevalence in Jiangsu Province in 2011].

Objective: To analyze the malaria prevalence and epidemic characteristics in Jiangsu Province in 2011 so as to provide a basis for drawing up and adjusting effective malaria elimination strategies and measures.

Methods: The reported malaria cases from the Internet reporting system and epidemiological data of malaria were collected and analyzed statistically in Jiangsu Province.

Results: A total of 374 malaria cases were reported in Jiangsu Province in 2011 with the incidence of 0.

[Establishment of early warning system of malaria epidemic situation in Jiangsu Province I impact of temperature on development of Plasmodium vivax in Anopheles sinensis in Jiangsu Province].

Objective: To observe the developmental threshold temperature and the effective accumulated temperature of Plasmodium vivax sporozoites in Anopheles sinensis in Jiangsu Province, and to analyze the impact of temperature on the development of Plasmodium vivax.

Methods: The Anopheles sinensis mosquitoes were maintained under different temperatures of 16 +/- 0.5, 19 +/- 0.

[Detection of Plasmodium sporozoites in mosquitoes by using fluorescent quantitative PCR].

Objective: To establish a fluorescent quantitative PCR (FQ-PCR) method for quantitative detection and species identification of Plasmodium sporozoites in Anopheles mosquitoes.

Methods: One pair of human Plasmodium genus-specific primers based on 18S rRNA genes were used and the reaction system and reaction condition of FQ-PCR were optimized by using the mixture of Plasmodium 18S rRNA gene recombinant plasmids and Anopheles DNA as a template. The specificity was verified by using four Plasmodium spp.

[Analysis of seasonal variability of vivax malaria by circular distribution method in Jiangsu Province from 1961 to 2010].

Objective: To study the law of seasonal variability of vivax malaria in Jiangsu Province from 1961 to 2010, so as to provide the reference for improving malaria control.

Methods: The data of vivax malaria incidence from the epidemic reporting system of Jiangsu Province were collected and analyzed to understand the month morbidity, peak prevalence time and mean durations, and the peak incidence durations every 10 years with the circular distribution method.

Results: Vivax malaria appeared obvious seasonal variability (P < 0.

[Field application of multiplex PCR to distinguish Plasmodium vivax and Plasmodium falciparum].

Objective: To explore the application value of multiplex PCR in the diagnosis of malaria in field.

Methods: The plasmodium genus-specific primer, Plasmodium vivax, P. falciparum species-specific primers were synthesis based on the specific target segments of small subunit of 18 S rRNA ribosomal.