Chlorophyll dephytylase 1 and chlorophyll synthase: a chlorophyll salvage pathway for the turnover of photosystems I and II.

Chlorophyll (Chl) is made up of the tetrapyrrole chlorophyllide and phytol, a diterpenoid alcohol. The photosynthetic protein complexes utilize Chl for light harvesting to produce biochemical energy for plant development. However, excess light and adverse environmental conditions facilitate generation of reactive oxygen species, which damage photosystems I and II (PSI and PSII) and induce their turnover.

Chlorophyll dephytylation in chlorophyll metabolism: a simple reaction catalyzed by various enzymes.

Chlorophyll (Chl) is composed of a tetrapyrrole ring and a phytol tail, which facilitate light energy absorbance and assembly with photosynthetic protein complexes, respectively. Chl dephytylation, the hydrolytic removal of the phytol tail, is considered a pivotal step in diverse physiological processes, such as Chl salvage during repair of the photosystem, the Chl cycle in the adjustment of antenna size, and Chl breakdown in leaf senescence and fruit maturation. Moreover, phytol is a component of the tocopherols, a major form of vitamin E that is essential in the human diet.

The maize B chromosome is capable of expressing microRNAs and altering the expression of microRNAs derived from A chromosomes.

Supernumerary B chromosomes (Bs) are nonessential chromosomes that are considered genetically inert. However, the maize B carries control elements that direct its behavior, such as that of nondisjunction, during the second pollen mitosis, and affects normal A chromosomes during cell division. Recently, the maize B has been found to contain transcriptionally active sequences and to affect the transcription of genes on A chromosomes.

Supraoptimal activity of CHLOROPHYLL DEPHYTYLASE1 results in an increase in tocopherol level in mature arabidopsis seeds.

Tocopherols are synthesized in photosynthetic organisms, playing a role in plant stress tolerance. Recent studies showed that the phytol moiety of tocopherols comes from the salvaged phytol chain during chlorophyll degradation. However, the enzyme(s) responsible for chlorophyll dephytylation remains unclear.

Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis.

Chlorophyll turns over in green organs during photosystem repair and is salvaged via de- and rephytylation, but the enzyme involved in dephytylation is unknown. We have identified an Arabidopsis thaliana thylakoid protein with a putative hydrolase domain that can dephytylate chlorophyll in vitro and in vivo. The corresponding locus, CHLOROPHYLL DEPHYTYLASE1 (CLD1), was identified by mapping a semidominant, heat-sensitive, missense allele (cld1-1).

Cytomolecular characterization and origin of de novo formed maize B chromosome variants.

B chromosomes are dispensable elements that occur in many species, including maize. The maize B chromosome is acrocentric and highly heterochromatic and undergoes nondisjunction during the second pollen mitosis. In this study, we determined the genetic behavior and organization of two naturally occurring B chromosome variants (designated B(ta) and B(tb)).

Analysis of an Arabidopsis heat-sensitive mutant reveals that chlorophyll synthase is involved in reutilization of chlorophyllide during chlorophyll turnover.

Chlorophylls, the most abundant pigments in the photosynthetic apparatus, are constantly turned over as a result of the degradation and replacement of the damage-prone reaction center D1 protein of photosystem II. Results from isotope labeling experiments suggest that chlorophylls are recycled by reutilization of chlorophyllide and phytol, but the underlying mechanism is unclear. In this study, by characterization of a heat-sensitive Arabidopsis mutant we provide evidence of a salvage pathway for chlorophyllide a.

Characterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10L translocations.

Maize B chromosome sequences have been previously cloned by microdissection, and all are proven to be highly repetitive, to be homologous to the normal complement, and to show no similarity to any published gene other than mobile elements. In this study, we isolated sequences from defined B regions. The strategy involved identification and then mapping of AFLP-derived B fragments before cloning.