Orthologous mammalian A3A-mediated single-nucleotide resolution sequencing of DNA epigenetic modification 5-hydroxymethylcytosine.

Epigenetic modifications in genomes play a crucial role in regulating gene expression in mammals. Among these modifications, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are recognized as the fifth and sixth nucleobases in genomes, respectively, and are the two most significant epigenetic marks in mammals. 5hmC serves as both an intermediate in active DNA demethylation and a stable epigenetic modification involved in various biological processes.

Comprehensive analysis of transplacental transfer of environmental pollutants detected in paired maternal and cord serums.

Prenatal exposure to hazardous environmental pollutants is a critical global concern due to their confirmed presence in umbilical cord blood, indicating the ability of pollutants to cross the placental barrier and expose the fetus to harmful compounds. However, the transplacental transfer efficiencies (TTEs) of many pollutants remain underexplored. Herein, we developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantitatively analyze 91 environmental pollutants, including 13 bisphenols (BPs), 18 organophosphorus flame retardants (OPFRs), 7 brominated and other flame retardants (BFRs), 34 phthalates (PAEs), and 19 per- and polyfluoroalkyl substances (PFASs), in paired maternal and cord serums.

Healthy aging: A bibliometric analysis of the literature.

Due to dramatic growth of the aging population worldwide, there has been an urgent call for a public health strategy to manage healthy aging, with the ultimate goal being advancement of aging research. Considerable progress has been made in uncovering the mystery of aging process using multidisciplinary methods. There is a growing consensus in the field that aging traits which were originally thought to be disparate are likely to be interconnected.

Chinese version of the clinical learning environment comparison survey: Assessment of reliability and validity.

Background: Simulation is recommended as a substitute for clinical practice among nursing students. No current guidelines exist regarding the accurate percentage of simulation hours versus clinical practice hours. Comparing simulation with clinical practice is needed so that both strategies can be optimally combined in nursing education.

[Comparison of the effect of eight kinds of volatile oil of Chinese material medica on percutaneous absorption of ibuprofen in vitro].

Objective: To compare the permeation-enhancing effect of the volatile oil of eight kinds of Chinese material medica including Fructus Evodia, Radix Saposhnikoviae, Rhizoma Atractylodes lancea Radix Aucklandiae, Radix Curcuma wenyujin, Rhizoma et Radix Notopterygii, Lignum Aquilariae Resinatum, Herba Schizonepetae on the model drug of ibuprofen in order to provide theoretic foundation for the selection and application of natural penetration enhancer.

Methods: The permeation flux of ibuprofen was determined using the penetration experiment apparatus in vitro. The content of ibuprofen was measured by HPLC.