Expanding the utilization of binding pockets proves to be effective for noncovalent small molecule inhibitors against SARS-CoV-2 M.

The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in millions of deaths and continues to pose serious threats to global public health. The main protease (M) of SARS-CoV-2 is crucial for viral replication and its conservation, making it an attractive drug target. Here, we employed a structure-based drug design strategy to develop and optimize novel inhibitors targeting SARS-CoV-2 M.

The Application of 3D-Printed Microporous Laminae in Kyphosis Correction Surgery for Ankylosing Spondylitis: Minimum Two-Year Follow-Up.

Study Design: A retrospective study.

Objective: This study designed an innovative 3D-printed microporous lamina and aimed to evaluate the feasibility and effectiveness in kyphosis correction surgery for patients with ankylosing spondylitis (AS).

Summary Of Background Data: Spinal osteotomy is a widely employed treatment for severe kyphotic deformities in AS.

Bacterial volatiles from aphid honeydew mediate ladybird beetles oviposition site choice.

Background: The cotton-melon aphid Aphis gossypii Glover is a destructive pest worldwide that causes substantial damage to diverse crops. The ladybird beetle Propylea japonica Thunberg is the dominant predatory natural enemy of A. gossypii.

AMPK activation by hepatitis E virus infection inhibits viral replication through attenuation of autophagosomes and promotion of innate immunity.

Hepatitis E virus (HEV) infection is generally asymptomatic or leads to acute and self-limiting hepatitis. The mechanisms orchestrating such an infection course remain to be elucidated. AMP-activated protein kinase (AMPK) is a pivotal cellular sensor for maintaining metabolic homeostasis.

Frozen CLIP-DINO: a Strong Backbone for Weakly Supervised Semantic Segmentation.

Weakly supervised semantic segmentation has witnessed great achievements with image-level labels. Several recent approaches use the CLIP model to generate pseudo labels for training an individual segmentation model, while there is no attempt to apply the CLIP model as the backbone to directly segment objects with image-level labels. In this paper, we propose WeCLIP and its advanced version WeCLIP+, to build the single-stage pipeline for weakly supervised semantic segmentation.

E2F1 activates USP19 to affect the stability of c-Myc to facilitate the progression of hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) is the most common malignant tumor worldwide with a high mortality rate. Herein, this study aims to explore the molecular mechanisms of E2F transcription factor 1 (E2F1), ubiquitin specific peptidase 19 (USP19) and c-Myc in regulating HCC progression.

Methods: RT-qPCR and western blotting were utilized to assess mRNA and protein expression, respectively.

The Flexible Role of Social Experience in the Processing of Abstract Concepts.

Multiple representation theories propose that social experience plays an important role in grounding abstract concepts. However, it is less clear how social experience influences the processing of abstract concepts, especially whether this influence is modulated by emotional experience and task demands. To address this question, we orthogonally manipulated the socialness (high vs.

Genetic structure and designing a preliminary core collection of in China based on 12 microsatellites markers.

The genetic diversity and structure of wild crop relatives are crucial for their conservation and utilization in breeding programs. This study presents a comprehensive survey and collection of across its natural distribution range in China. Using 12 microsatellite markers, the genetic diversity of 357 wild accessions from 25 populations was evaluated, revealing a high genetic diversity ( = 0.

Knowledge, attitudes, and practices regarding food-medicine dual-purpose substances among adults in China: a cross-sectional online survey.

Background: Food medicine homology originated in ancient China and has been officially named food-medicine dual-purpose substances (FMS). However, the relationship between residents' knowledge, attitudes, practices (KAP) and FMS among adults in China remains unclear. This study evaluated the current situation of FMS-related knowledge, awareness and actual consumption behaviors and practices of Chinese residents to provide a basis for both perfect related regulations and scientific advice on FMS consumption.

Compatibility Studies of Different Crystal Forms of Imatinib Mesylate with Pharmaceutical Excipients.

Imatinib mesylate has been identified as a tyrosine kinase inhibitor that selectively inhibits Abl tyrosine kinases, including Bcr-Abl. It exhibits many polymorphic forms, with the most stable and commercialized polymorphs known as the α and β forms. In the present study, the compatibility between the polymorphs of Imatinib mesylate and selected excipients (MCC, HPMC, PVPP, SiO, MS, and CaHPO) was investigated by differential scanning calorimetry (DSC), Raman spectra, X-ray diffraction (XRD) technique, and isothermal stress testing (IST).

A Flexible-Integrated Multimodal Hydrogel-Based Sensing Patch.

Sleep monitoring is an important part of health management because sleep quality is crucial for restoration of human health. However, current commercial products of polysomnography are cumbersome with connecting wires and state-of-the-art flexible sensors are still interferential for being attached to the body. Herein, we develop a flexible-integrated multimodal sensing patch based on hydrogel and its application in unconstraint sleep monitoring.

p23 protects against ferroptosis of brain microvascular endothelial cells in ischemic stroke.

Ferroptosis is a type of iron‑dependent regulated cell death that differs from apoptosis, autophagy or necrosis. p23 serves as a co‑chaperone and performs a unique biological function in various diseases by binding to client proteins to modulate their biological functions; however, its effect on ferroptosis remains largely unknown. In the present study, the effects of cerebral ischemia/reperfusion (I/R) injury (CIRI) or oxygen‑glucose deprivation/reoxygenation on the blood‑brain barrier (BBB) and ferroptosis in brain microvascular endothelial cells (BMECs), as well as the expression of p23, were examined.

Iconic gestures facilitate language comprehension in consecutive interpreting.

Previous studies have shown that iconic gestures can be integrated with speech for better comprehension in both first and second language contexts. Yet it remains unclear whether such speech-gesture integration is found in consecutive interpreting (CI) and influenced by language tasks (passive viewing or CI) and interpreting experience (individuals with or without CI experience). To this end, we conducted two experiments through a modified cued-recall task in which Chinese-English bilinguals were exposed to utterances accompanied by three gesture conditions: semantically related gesture, semantically unrelated gesture, and no gesture.

Advanced External Beam Stereotactic Radiotherapy for Skull Base Reirradiation.

Background/objectives: Stereotactic body radiation therapy (SBRT) for skull base reirradiation is particularly challenging, as patients have already received substantial radiation doses to the region, and nearby normal organs may have approached their tolerance limit from prior treatments. In this study, we reviewed the characteristics and capabilities of four advanced external beam radiation delivery systems and four modern treatment planning systems and evaluated the treatment plan quality of each technique using skull base reirradiation patient cases.

Methods: SBRT plans were generated for sixteen skull base reirradiation patients using four modalities: the GK plan for the Elekta Leksell Gamma Knife Perfexion/ICON, the CyberKnife (CK) plan for the Accuray CyberKnife, the intensity-modulated proton therapy (IMPT) plan for the Hitachi ProBeat-FR proton therapy machine, and the volumetric-modulated arc therapy (VMAT) plan for the Varian TrueBeam STx.

A frequency attention-embedded network for polyp segmentation.

Gastrointestinal polyps are observed and treated under endoscopy, so there presents significant challenges to advance endoscopy imaging segmentation of polyps. Current methodologies often falter in distinguishing complex polyp structures within diverse (mucosal) tissue environments. In this paper, we propose the Frequency Attention-Embedded Network (FAENet), a novel approach leveraging frequency-based attention mechanisms to enhance polyp segmentation accuracy significantly.

Characterization and Identification of the Metabolites of Tuxobertinib in Rat, Dog, Monkey, and Human Liver Microsomes by Liquid Chromatography Combined With High Resolution Mass Spectrometry.

Tuxobertinib is an EGFR tyrosine kinase inhibitor, which was undergoing clinical development for the treatment of non-small cell lung cancer (NSCLC). The aim of this study was to characterize the metabolites of tuxobertinib in liver microsomes and recombinant cytochrome P450 enzymes and to propose the metabolic pathways. The metabolites were generated by individually incubating tuxobertinib (5 μM) with rat, dog, monkey, and human liver microsomes, NADPH, and at 37°C for 60 min.

Mitigation of stimulated Brillouin scattering in inhomogeneous plasmas by broadband lasers.

A theoretical model of stimulated Brillouin scattering (SBS) developed by a broadband laser in inhomogeneous plasmas is proposed and further investigated through a series of numerical simulations using the low-coherence laser model of the Kunwu facility. Polychromatic beamlets are coupled to develop a scattering mode, when all the poles of the beamlets are on the integration path of SBS dispersion relations. The coupled beamlets can be regarded as a monochromatic beam with the same incident energy, owing to their nearly identical amplification coefficients.

Strain Release via Glass Transition Temperature Regulation for Efficient and Stable Perovskite Solar Cells.

Thermally induced tensile strain that remains in perovskite films after annealing is one of the key reasons for diminishing the performance and operational stability of perovskite solar cells (PSCs). Herein, a glass transition temperature (T) regulation (TR) strategy is developed by introducing two polymerizable monomers, 2-(N-3-Sulfopropyl-N,N-dimethyl ammonium)ethyl methacrylate (SBMA) and 2-Hydroxyethyl acrylate (HEA), into the perovskite layer. SBMA and HEA undergo in situ polymerization, which regulates the nucleation and crystal growth of the perovskite film.

The complete chloroplast genome and phylogenetic analysis of (Meisn.) Migo (Polygonaceae).

is a plant distributed at meadow or wetland. Our study reports the complete chloroplast genome. The chloroplast genome of is a typical tetrameric structure with a total length of 159,843 bp, containing a large single-copy (LSC) region of 84,350 bp, a small single-copy (SSC) region of 13,151 bp, and two inverted repeat regions (IRs) of 31,171 bp.

Multifunctional Graphdiyne Enables Efficient Perovskite Solar Cells via Anti-Solvent Additive Engineering.

Finding ways to produce dense and smooth perovskite films with negligible defects is vital for achieving high-efficiency perovskite solar cells (PSCs). Herein, we aim to enhance the quality of the perovskite films through the utilization of a multifunctional additive in the perovskite anti-solvent, a strategy referred to as anti-solvent additive engineering. Specifically, we introduce ortho-substituted-4'-(4,4″-di-tert-butyl-1,1':3',1″-terphenyl)-graphdiyne (o-TB-GDY) as an AAE additive, characterized by its sp/sp-cohybridized and highly π-conjugated structure, into the anti-solvent.

Study on Fermentation Preparation, Stability, and Angiotensin-Converting Enzyme Inhibitory Activity of Tomato Pomace Peptide.

The substantial quantity of discarded tomato pomace (TP) results in the waste of valuable resources. This study utilizes these tomato by-products by mixing them with water in a specific proportion and fermenting the mixture in two stages: first with yeast, and then with lactic acid bacteria. The most suitable microbial strains for TP fermentation were identified by evaluating parameters such as peptide content, degree of hydrolysis, and gel electrophoresis analysis.

Composite Graphene for the Dimension- and Pore-Size-Mediated Stem Cell Differentiation to Bone Regenerative Medicine.

As one of the most promising means to repair diseased tissues, stem cell therapy with immense potential to differentiate into mature specialized cells has been rapidly developed. However, the clinical application of stem-cell-dominated regenerative medicine was heavily hindered by the loss of pluripotency during the long-term in vitro expansion. Here, a composite three-dimensional (3D) graphene-based biomaterial, denoted as GO-Por-CMP@CaP, with hierarchical pore structure (micro- to macropore), was developed to guide the directional differentiation of human umbilical cord MSCs (hucMSCs) into osteoblasts.

Descriptive epidemiology and prognostic factors of atypical teratoid/rhabdoid tumors in the United States, 2001-2021.

Atypical teratoid rhabdoid tumor (AT/RT) is a rare embryonal central nervous system tumor with a dismal prognosis that occurs mostly in early childhood. Since recent epidemiological and prognostic information is limited, we aimed to describe and analyze AT/RT-related incidences, temporal trends and prognostic factors. Incidence and survival data between 2001 and 2021 were extracted from the Surveillance, Epidemiology, and End Results (SEER) database.

Impact of triclocarban exposure on development and gene expression in the wolf spider, Pardosa pseudoannulata (Araneae: Lycosidae).

Triclocarban (TCC), an emerging organic contaminant, poses a potential threat to water resources and ecosystems. The wolf spider Pardosa pseudoannulata (Araneae: Lycosidae) is a dominant predator typically inhabiting rice fields or wet habitats near water sources. However, little is known about the effects of TCC on the wolf spiders.