Epithelial FETUB-mediated the inhibition of NEP activity aggravates asthma.

Background: Neuropeptide accumulation exacerbates asthma, with reduced neprilysin (NEP) activity implicated. However, this regulatory mechanism remains unexplored.

Objective: To identify and characterize epithelial-derived modulators of NEP activity and their role in asthma pathogenesis.

Characterization and Mechanism Study of a Novel Ethanol Acetyltransferase from (EatH) with Good Thermostability, pH Stability, and Broad Alcohol Substrate Specificity.

Ethyl acetate, one of the most essential industrial compounds, has a broad range of applications, including flavors, fragrances, pharmaceuticals, cosmetics, and green solvents. Eat1 is accountable for bulk ethyl acetate production in yeasts, yet its properties and molecular mechanism are not well characterized. In this study, an gene from was obtained through gene mining.

Chrysin ameliorates dextran sulfate-induced ulcerative colitis in mice by modulating inflammation and gut microbiota.

Background: Inflammatory bowel disease (IBD) encompasses chronic inflammation of the colon and rectum, posing significant health challenges. Previous studies have shown potential therapeutic effects of natural compounds on IBD. Chrysin, a naturally occurring flavonoid, has been suggested to modulate inflammatory pathways and gut microbiota, but its comprehensive impact on ulcerative colitis remains inadequately explored.

MOTS-c Promotes Glycolysis via AMPK-HIF-1α-PFKFB3 Pathway to Ameliorate CPB-induced Lung Injury.

Cardiopulmonary bypass (CPB) is essential during cardiac surgeries but frequently leads to lung ischemia-reperfusion injury (LIRI), a significant contributor to postoperative complications. This study investigated the protective effects of MOTS-c, a mitochondrial-derived peptide, against LIRI-induced acute lung injury (ALI), emphasizing glycolytic reprogramming and ferroptosis in pulmonary microvascular endothelial cells (PMVECs). We hypothesized that MOTS-c exerts its protective effects by regulating glycolysis and suppressing ferroptosis via metabolic signaling pathways.

Adaptive Bit Selection for Scalable Deep Hashing.

Deep Hashing is one of the most important methods for generating compact feature representation in content-based image retrieval. However, in various application scenarios, it requires training different models with diversified memory and computational resource costs. To address this problem, in this paper, we propose a new scalable deep hashing framework, which aims to generate binary codes with different code lengths by adaptive bit selection.

Clinicopathological characteristics and prognostic significance of HER2 status evaluation in patients with urothelial carcinoma: a retrospective single-center experience in China.

Due to the lack of an in-depth evaluation of urothelial carcinoma (UC) HER2 expression in both primary and recurrent UC, we examined possible clinical and pathological variables associated with altered UC HER2 expression. The HER2 status and clinicopathological features of primary UCs and matched recurrences from 2015 to 2023 (n = 1352) were compared by evolution category (stable or altered). In 1352 UC patients, HER2 protein expression was linked with age, histological grade, and recurrence.

Electrochemical reduction boosted Luminol cathodic electrochemiluminescence for trace chiral recognition of alanine enantiomers.

Electrochemiluminescence (ECL) is highly recommended in chiral recognition. ECL-based chiral sensors highly desire a sensitive sensing interface for signal conversion and absolute chiral discrimination. The ECL emission based on a luminol-dissolved O system received much attention due to its nontoxicity and stability.

The Molecular Mechanism of a Complex1-Induced Apoptosis in Cancer Cells of the Esophagus.

Background: Esophageal Cancer (EC) is a commonly occurring cancer of the digestive tract. The bismuth compounds from thiosemicarbazones have been observed to be active against cancer cells. However, a synthetic nine-coordinate bismuth (III) complex (complex 1) has never been assessed so far for its anticancer in the esophageal squamous cell carcinoma cell line (EC109).

Induction of enhanced stem-directed neutralizing antibodies by HA2-16 ferritin nanoparticles with H3 influenza virus boost.

Current seasonal influenza vaccines offer limited protection against influenza viruses due to genetic drift. The urgent need for a universal influenza vaccine to combat highly mutated strains is evident. This study utilized the conserved HA2 subunit of hemagglutinin (HA) and a short linear epitope of HA2 (HA2-16) from the H3 influenza virus to conjugate with ferritin, resulting in the construction of recombinant immunogens termed HA2-F and HA2-16-F, respectively.

Prevalence and clinical correlates of chronic obstructive pulmonary disease in heart failure patients: a cross-sectional study in China.

Background: Despite chronic obstructive pulmonary disease's (COPD)'s prevalence in the general populace, its incidence in heart failure (HF) patients is understudied. This study aimed to assess COPD prevalence and clinical associations in Chinese HF patients.

Methods: From the Chinese Heart Failure Study, demographic and clinical details of 2008 HF patients were analyzed.

Effect of cavity absorption on treatment outcomes of Mycobacterium kansasii pulmonary disease.

Background: The incidence of Mycobacterium kansasii pulmonary disease (MK-PD) was increasing worldwide. However, limited research was conducted on how to improve the cure rate and evaluate treatment efficacy for patients. We aimed to identify key factors that could improve the cure rate in patients with MK-PD.

Elderly vulnerability to temperature-related mortality risks in China.

The elderly face elevated mortality risk due to rising temperature. Previous assessments of temperature-related mortality, however, lack a comprehensive analysis of distinct impacts of temperature change across different timescales and characteristics. Using a longitudinal survey of 27,233 elderly Chinese citizens from 2005 to 2018, we establish connections between rising temperatures, temperature variability, and extreme heat with increased mortality risk, assessed through four annual metrics that combine temperature and humidity.

Incompatible Geometry Regulation of Nanowire Assemblies Enabled Light-Driven Shape Morphing and Motions.

Photoresponsive shape-changing materials have significant applications in miniaturized smart robotics and biomedicine powered in a remote and wireless manner. Existing light-fuelled soft materials suffer from limited continuous shape manipulation and constrained mobility and locomotive modes. One promising solution is developing a hierarchical structure design approach to integrate rapid, reversible photoactive molecular alignment and mechanically incompatible geometry in a macroscopic system.

Preparation of protein-resistant magnetic molecularly imprinted polymers as solid-phase extraction adsorbents via a one-stone-two-birds strategy for selective enrichment of tetracycline in milk.

The widespread presence of antibiotic residues in animal-derived foods poses serious risks to public health and the environment, emphasizing the need for urgent action. This study presented a novel lightweight, popcorn-like magnetic molecularly imprinted polymer (LPR-MMIPs) with protein-resistant properties, for the selective enrichment of tetracycline (TC) antibiotics in milk. During the preparation of LPR-MMIPs, bovine serum albumin (BSA) was used as the functional monomer, and tris(2-carboxyethyl)phosphine (TCEP) served as the reducing agent.

Risk assessment and management strategy of two new NDSRIs in a pharmaceutical drug product for the treatment of a rare disease: From prediction to control.

N-nitrosamines are a class of compounds belonging to the "cohort of concern" and characterized by the linkage of a nitroso group (-N=O) to an amine functional group (-NR). Some of these compounds are mutagenic, genotoxic, and potentially carcinogenic agents in humans, which necessitates control at acceptable safe levels. The current work presents a comprehensive risk assessment and mitigation strategy for two complex diastereomeric nitrosamines as New Drug Substance Related Impurities (NDSRIs) for miglustat 65mg capsules.

A phase 1 trial of prizloncabtagene autoleucel, a CD19/CD20 CAR T-cell therapy for relapsed/refractory B-cell non-Hodgkin lymphoma.

Prizloncabtagene autoleucel (prizlon-cel), a novel bispecific chimeric antigen receptor (CAR) T-cell, targets and eliminates CD19/CD20 positive tumor cells. This phase 1, open-label study investigated the safety and efficacy of prizlon-cel in patients with relapsed/refractory B-cell non-Hodgkin Lymphoma (r/r B-NHL). Patients with CD19 and/or CD20-positive r/r B-NHL received a 3-day lymphodepletion (cyclophosphamide: 300 mg/m2/d; fludarabine: 30 mg/m2/d) followed by an intravenous dose of prizlon-cel.

Genetically predict the association between 91 human blood cell perturbation phenotypes and IBD: A Mendelian randomization study.

Inflammatory bowel disease (IBD), encompassing Crohn disease and ulcerative colitis, is a group of persistent and recurrent gastrointestinal disorders. Despite the prevalence of these conditions, no studies have been conducted to examine the connection between altered human blood cell phenotypes and the underlying mechanisms of IBD pathogenesis. By utilizing summary statistics from genome-wide association studies, we executed a systematic two-sample Mendelian randomization (MR) investigation on 91 genetically determined blood cell perturbation traits in relation to 3 separate IBD phenotypes.

Fruquintinib Plus Sintilimab in Patients with Treatment-Naive and Previously Treated Advanced Renal Cell Carcinoma: Results from a Phase Ib/II Clinical Trial.

Background: Antiangiogenic inhibitors plus immune checkpoint inhibitors have synergistic antitumor activity and have improved treatment outcomes in patients with renal cell carcinoma (RCC).

Objective: We report the RCC cohort from a phase Ib/II study in Chinese patients evaluating the efficacy and safety of fruquintinib plus sintilimab in treating advanced clear cell RCC (ccRCC).

Patients And Methods: Eligible patients had pathologically confirmed advanced ccRCC.

The Dual Role of Cellular Senescence in Macrophages: Unveiling the Hidden Driver of Age-Related Inflammation in Kidney Disease.

Aging is a complex biological process that involves the gradual decline of cellular, tissue, and organ functions. In kidney, aging manifests as tubular atrophy, glomerulosclerosis, and progressive renal function decline. The critical role of senescence-associated macrophage in diseases, particularly kidney diseases, is increasingly recognized.

TLE1 corepressor promotes gefitinib resistance in lung cancer A549 cells via E‑cadherin silencing.

As a putative lung specific oncogene, the transducin-like enhancer of split 1 (TLE1) corepressor drives an anti-apoptotic and pro-epithelial-mesenchymal transition (EMT) gene transcriptional programs in human lung adenocarcinoma (LUAD) cells, thereby promoting anoikis resistance and tumor aggressiveness. Through its survival- and EMT-promoting gene regulatory programs, TLE1 may impact drug sensitivity and resistance in lung cancer cells. In the present study, a novel function of TLE1 was uncovered as an inhibitor of the antitumor effects of the epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) gefitinib in the human LUAD cell line A549, which exhibits moderate sensitivity to EGFR-TKI.

Hierarchically aligned heterogeneous core-sheath hydrogels.

Natural materials with highly oriented heterogeneous structures are often lightweight but strong, stiff but tough and durable. Such an integration of diverse incompatible mechanical properties is highly desired for man-made materials, especially weak hydrogels which are lack of high-precision structural design. Herein, we demonstrate the fabrication of hierarchically aligned heterogeneous hydrogels consisting of a compactly crosslinked sheath and an aligned porous core with alignments of nanofibrils at multi-scales by a sequential self-assembly assisted salting out method.

Genome-wide identification and gene expression pattern analysis of the glycoside hydrolase family 1 in Fagopyrum tataricum.

Background: The β-glucosidases (BGLU) of glycoside hydrolase family 1 hydrolyze the glycosidic bond to release β-D-glucose and related ligands, which are widely involved in important physiological processes in plants. Genome-wide analysis of the BGLU genes in the model crops Arabidopsis thaliana and Oryza sativa revealed that they are functionally diverse. In contrast, the BGLU gene family in Tartary buckwheat remains unclear.

Network pharmacology and anticancer mechanism study of Dendrobium nobile dendrobine in the treatment of colorectal cancer.

Objective: This study aims to explore the potential targets and anticancer mechanisms of dendrobine from Dendrobium nobile in the treatment of colorectal cancer through network pharmacology, and to experimentally validate its specific effects.

Methods: Initially, potential targets of dendrobine were identified using the ITCM Traditional Chinese Medicine database, while colorectal cancer-related genes were obtained from the NCBI Gene database, with the intersection of these datasets taken for further analysis. Functional enrichment analysis was conducted using the Metascape database, and a protein-protein interaction (PPI) network was constructed.