Gentiopicroside targeting AKT1 activates HIF-1α/VEGF axis promoting diabetic ulcer wound healing.

Backgound: Gentiopicroside (GSP) have been proven to accelerate the healing of diabetic ulcers (DU), but the underlying molecular mechanisms remain unclear. This study aims to explore the mechanism by which GSP accelerates the healing of DU.

Method: The targets of GSP were firstly predicted using the SuperPred, SwissTargetPrediction, and Pharmmapper databases; DU-related transcriptome data were obtained from the GEO database, including GSE147890, GSE68183, and GSE199939; differential expression analysis was conducted using the Limma package, and DU-related targets were identified after summarization and de-duplication.

Comparative Analysis of Egg Yolk Phospholipid Unsaturation and Its Impact on Neural Health in Alzheimer Disease Mice.

The mechanism of egg yolk phosphatidylcholine (PC) in alleviating Alzheimer's disease (AD) has not yet been clear. The fatty acid composition of PC, especially the ratio of polyunsaturated fatty acids (PUFA), may be a critical determinant of their structural and functional roles. This study aimed to conduct a comparative analysis of the unsaturation levels of egg yolk PC and their impact on neurological health in a murine model of AD.

The Role of Primary Cilia in Modulating the Luteinization Process of Ovarian Granulosa Cells in Mice.

The corpus luteum is the principal progesterone-secreting gland, while primary cilia function as pivotal organelles in intercellular signal transduction. Together, they play an essential role in the establishment and maintenance of pregnancy. However, the mechanisms underlying the role of primary cilia in granulosa cell luteinization in mouse ovaries remain poorly understood.

Residential greenness, air pollution, genetic predisposition and the risk of lung cancer.

Background: Growing evidence suggests that greenness is associated with multiple health outcomes, but its association with lung cancer risk remains limited and far from consistent. In particular, it is unclear whether this association varies across different types of lung cancer and whether it is influenced by factors such as genetics, air pollution, and physical activity.

Method: This prospective cohort study included 425108 participants aged 37-73 years from the UK Biobank.

UK Biobank data demonstrate long-term exposure to floods is a risk factor for incident dementia.

Background: Flooding has emerged as the most prevalent natural disaster, impacting billions of individuals worldwide. However, the long-term effects of flooding exposure on dementia remain unclear.

Methods: With a nested case-control design, a risk-set sampling method was used to match cases and controls.

Enhancing SLAM algorithm with Top-K optimization and semantic descriptors.

To address the computational challenges faced by edge devices using deep learning to process LiDAR point cloud data, this paper proposes a SLAM algorithm incorporating Top-K optimization to generate semantic descriptors and global semantic map for laser data efficiently. This approach aims to reduce computational complexity while enhancing processing speed. The algorithm extracts semantic information from LiDAR data, constructs two-dimensional semantic descriptors, and improves the robot's semantic understanding of its surrounding environment.

Deep reinforcement learning trajectory planning for robotic manipulator based on simulation-efficient training.

The paper proposes a new M2ACD(Multi-Actor-Critic Deep Deterministic Policy Gradient) algorithm to apply trajectory planning of the robotic manipulator in complex environments. First, the paper presents a general inverse kinematics algorithm that transforms the inverse kinematics problem into a general Newton-MP iterative method. The M2ACD algorithm based on multiple actors and critics is structured.

Terahertz Spin-to-Charge Conversion in Strontium Iridate-Based Magnetic Heterostructures.

Oxide interfaces have enormous potential for future electronics with many applications, such as large spin Hall conductance, phase transitions, topological states, and superconductivity. However, previous investigations have predominantly focused on gigahertz frequencies; whilst the possibilities to fabricate devices operational at terahertz frequencies are demonstrated. A model solution is proposed employing 5d rare-earth, strontium iridate (SrIrO) heterostructure with cobalt (Co) ultrathin layers.

Evolution of the Asian summer monsoon and regional karst ecological environment since the middle ages in Southwest China.

The frequent droughts and floods, closely associated with the Asian summer monsoon (ASM), has profoundly affected the ecological environment and economy in East Asia. While changes in the ASM are closely related to precipitation patterns, the specific mechanism still requires further investigation. This study utilized stalagmite records from the Feilong Cave in southwest China to reconstruct the evolution of the ASM since the Medieval Warm Period (MWP).

Dynamic Nomogram for Predicting the Fall Risk of Stroke Patients: An Observational Study.

Background: Common fall risk assessment scales are not ideal for the prediction of falls in stroke patients. The study aimed to develop and verify a dynamic nomogram model for predicting the falls risk in stroke patients during rehabilitation.

Methods: An observational study design was adopted, 488 stroke patients were treated in a tertiary hospital from March to September 2022 were investigated for fall risk factors and related functional tests.

Feasibility of Serum Galectin-1 as a Diagnostic Biomarker for Metabolic Dysfunction-Associated Steatotic Liver Disease: A Study on a Segment of the Chinese Population Using Convenience Sampling.

: Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) is commonly considered as a hepatic manifestation of metabolic syndrome, posing considerable public health and economic challenges due to its high prevalence. This study investigates the diagnostic potential of serum galectin-1 levels in MASLD patients. : A total of 128 participants were analyzed for this study, comprising 68 healthy controls and 60 MASLD patients.

Individualized tumor-reactive T cells exhibit a potent anti-tumor response in prostate cancer.

Background: Cellular immunotherapy exhibits promise in treating blood tumors. However, its application for solid tumors is impeded by their heterogeneity and complex microenvironments. The development of individualized multitarget therapy may be the key to overcoming the challenge of tumor heterogeneity.

Evaluation of twin-arginine translocation substrate proteins as potential antigen candidates for serodiagnosis of brucellosis.

Introduction: Brucellosis, an infectious zoonotic disease caused by members of the genus , results in chronic multi-organ injury. Improving the specificity and sensitivity of serological methods for diagnosing brucellosis necessitates the development of novel diagnostic antigens. The twin-arginine translocation (Tat) pathway is responsible for transporting folded proteins across the cytoplasmic membrane and has been implicated in the virulence of .

Placental quantitative susceptibility mapping and T2* characteristics for predicting birth weight in healthy and high-risk pregnancies.

Background: The human placenta is critical in supporting fetal development, and placental dysfunction may compromise maternal-fetal health. Early detection of placental dysfunction remains challenging due to the lack of reliable biomarkers. This study compares placental quantitative susceptibility mapping and T2* values between healthy and high-risk pregnancies and investigates their association with maternal and fetal parameters and their ability to predict birth weight (BW).

Metabolizable and net energy evaluation of corn, soybean meal, and wheat bran in growing male pheasants.

The apparent metabolizable energy(AME), AME corrected to zero-nitrogen retention(AMEn) and net energy (NE) values of corn, soybean meal and wheat bran were determined in growing male pheasants with reference diet substitution method. Reference diet was formulated according to standard Pheasants requirement, and test diets contained 40 % of corn, 20 % of soybean meal and 30 % of the wheat bran samples of thereference diet. Ninety male pheasants at the age of 12 wk with similar body weights were randomly divided into four groups.

Aromatic and sensorial profiles of Guichang kiwi wine fermented by indigenous Saccharomyces cerevisiae strains.

The indigenous Saccharomyces cerevisiae (S. cerevisiae) serves as an essential tool for enhancing the sensory and aromatic profiles of fruit wines from specific regions. This study aims to investigate the effects of inoculating indigenous S.

The clinical significance and biological function of tropomyosin 3 in ulcerative colitis.

Background: Ulcerative colitis (UC) is a lifelong chronic inflammatory disease that is characterized by the absence of specific markers for diagnosis and prognosis. TPM3 is an integral component of the thin filament, responsible for the structural stability of actin filaments and modulation of cytoskeletal function. This study investigated the regulatory role of TPM3 in UC and its potential mechanisms.

Carboxymethylated polysaccharides from Chrysanthemum indicum with antimicrobial and hemostatic properties for promoting wound healing.

Seeking potential active ingredients with suitable functions to promote the entire wound repair process remains an unmet requirement. In this study, the polysaccharide (CIP) with an average molecular weight of 5.85 × 10 g/mol was isolated from dried Chrysanthemum indicum flowers.

Investigating the molecular interactions of two long-chain PFASs with human serum albumin: Insights from multispectral analysis and computational methods.

Long-chain perfluoroalkyl substances (FPAS) are commonly detected in environmental and biological contexts. This study investigated the interactions of perfluoroundecanoic acid (PFUnDA) and perfluorotridecanoic acid (PFTrDA) with human serum albumin (HSA). Fluorescence quenching experiments demonstrated static quenching of HSA's intrinsic fluorescence by both PFUnDA and PFTrDA, resulting in the formation of stable HSA-PFAS complexes.

Energy-Confinement 3D Flower-Shaped Cages for AI-Driven Decoding of Metabolic Fingerprints in Cardiovascular Disease Diagnosis.

Rapid and accurate detection plays a critical role in improving the survival and prognosis of patients with cardiovascular disease, but traditional detection methods are far from ideal for those with suspected conditions. Metabolite analysis based on nanomatrix-assisted laser desorption/ionization mass spectrometry (NMALDI-MS) is considered to be a promising technique for disease diagnosis. However, the performance of core nanomatrixes has limited its clinical application.

Aroma analysis and biomarker screening of 27 tea cultivars based on four leaf color types.

Green is no longer the only color used to describe tea leaves. As tea plants with different leaf colors-white, yellow, and purple-yield significant economic benefits, scholars are growing increasingly curious about whether these differently colored leaves possess unique aromatic characteristics. Headspace solid-phase microextraction (HS-SPME) combined with GC-MS was used to analyze the volatile metabolites of buds and leaves from 7 white-leaf tea plants, 9 yellow-leaf tea plants, 4 purple-leaf tea plants, and 7 normal (green) tea plants.

Separating nanobubble nucleation for transfer-resistance-free electrocatalysis.

Electrocatalytic gas-evolving reactions often result in bubble-covered surfaces, impeding the mass transfer to active sites. Such an issue will be worsened in practical high-current-density conditions and can cause sudden cell failure. Herein, we develop an on-chip microcell-based total-internal-reflection-fluorescence-microscopy to enable operando imaging of bubbles at sub-50 nm and dynamic probing of their nucleation during hydrogen evolution reaction.

Theoretical study of the formation of HO by lytic polysaccharide monooxygenases: the reaction mechanism depends on the type of reductant.

Lytic polysaccharide monooxygenases (LPMOs) are a unique group of monocopper enzymes that exhibit remarkable ability to catalyze the oxidative cleavage of recalcitrant carbohydrate substrates, such as cellulose and chitin, by utilizing O or HO as the oxygen source. One of the key challenges in understanding the catalytic mechanism of LPMOs lies in deciphering how they activate dioxygen using diverse reductants. To shed light on this intricate process, we conducted in-depth investigations using quantum mechanical/molecular mechanical (QM/MM) metadynamics simulations, molecular dynamics (MD) simulations, and density functional theory (DFT) calculations.

An Ultrasensitive Duplex Aptasensor Featuring the Target-Triggered Lanthanide Luminescence Antenna Effect for Wash-Free Detection of Epithelial Cancerous Exosomes.

Fluorescence sensing is widely used in in vitro detection due to its high sensitivity and rapid result delivery. However, detection systems based on nanomaterials involving complex and cumbersome purification steps can lead to sample loss and significantly reduce the accuracy of the results. To address this issue, we proposed a lanthanide-based aptasensor featuring the target-triggered antenna effect to significantly enhance the time-resolved luminescence (TRL) of chelated Tb combined with a wash-free strategy.

Identifying the healthy places to live in Australia with a new environmental quality health index.

Background: Existing environmental quality indices often fail to account for the varying health impacts of different exposures and exclude socio-economic status indicators (SES).

Objectives: To develop and validate a comprehensive Environmental Quality Health Index (EQHI) that integrates multiple environmental exposures and SES to assess mortality risks across Australia.

Methods: We combined all-cause, cardiovascular, and respiratory mortality data (2016-2019) from 2,180 Statistical Areas Level 2 with annual mean values of 12 environmental exposures, including PM, ozone, temperature, humidity, normalized difference vegetation index, night light, road and building density, and socioeconomic status.