Altered LY6E and TRIM6 expression in PBMCs correlated with HBsAg clearance and response to Peg-IFN-α treatment in HBeAg-negative chronic hepatitis B patients.

Background: Pegylated interferon alpha (Peg-IFN-α) has the potential to eradicate hepatitis B surface antigen (HBsAg). This study aimed to investigate whether the expression levels of lymphocyte antigen 6 family member E (LY6E) and tripartite motif-containing protein 6 (TRIM6) mRNAs in peripheral blood mononuclear cells (PBMCs) of hepatitis B e antigen (HBeAg)-negative chronic hepatitis B virus (HBV) patients is associated with the response to Peg-IFN-α treatment and HBsAg clearance.

Methods: In this prospective study, HBeAg-negative chronic HBV patients treated with Peg-IFN-α were followed for 48 weeks.

Family Physicians' Use of, Barriers to, and Attitudes Toward Remote Diagnosis and Treatment in China: A National Web-Based Survey.

China has introduced a series of policies to encourage family physicians (FPs) to provide remote diagnosis and treatment (RDT) services, with the ultimate goal of providing more continuous, convenient, and efficient medical services for all residents, especially elderly patients and patients with chronic diseases. However, few studies have focused on this important issue. The aim of this study was to provide a comprehensive description of FPs' use of, barriers to, and attitudes toward RDT in China.

Association between estimated glucose disposal rate and incident cardiovascular disease in a population with Cardiovascular-Kidney-Metabolic syndrome stages 0-3: insights from CHARLS.

Objective: Estimated glucose disposal rate (eGDR) is a reliable marker of insulin resistance (IR), which has been proven to be strongly linked to cardiovascular and renal diseases. However, the link between eGDR and the occurrence of cardiovascular disease (CVD) in individuals exhibiting Cardiovascular-Kidney-Metabolic (CKM) syndrome stages 0-3 remains ambiguous.

Methods: The data employed in this investigation was procured from the China Health and Retirement Longitudinal Study (CHARLS).

Exosomal lncRNA Mir100hg from lung cancer stem cells activates H3K14 lactylation to enhance metastatic activity in non-stem lung cancer cells.

The mean survival of metastatic lung adenocarcinoma is less than 1 year, highlighting the urgent need to understand the mechanisms underlying its high mortality rate. The role of Extracellular vesicles (EVs) in facilitating the interactions between cancer cells and the metastatic microenvironment has garnered increasing attention. Previous studies on the role of EVs in metastasis have been primarily focused on cancer cell-derived EVs in modulating the functions of stromal cells.

FBXW7 Directly Ubiquitinates and Degrades CTNNB1 Mediating the Suppression of ENKUR in Endometrial Cancer.

Enkurin (ENKUR) is a tumor suppressor in some malignancies. However, its role in endometrial cancer (EC) remains unknown. Here, we firstly observed that reduced ENKUR expression promotes progression and poor prognosis in EC.

Generation of an induced pluripotent stem cell (iPSC) line (INNDSUi008-A) from a patient with Spinocerebellar Ataxia Type 3.

Abnormal trinucleotide CAG repeat expansions in exon 10 of the ataxin-3 (ATXN3) gene has been identified as the cause of Spinocerebellar Ataxia Type 3 (SCA3). We generated and characterized a human induced pluripotent stem cell (iPSC) line from skin fibroblasts of a patient with genetically confirmed SCA3. The pluripotency of these iPSCs was verified by the expression of several undifferentiated hPSCs markers at both RNA and protein levels, as well as their capability to differentiate into all three germ layers.

Experimental evidence-based construction of electroacupuncture for ischemic stroke: a meta-analysis and systematic review.

Objective: Ischemic stroke represents a leading cause of disability and mortality worldwide, necessitating effective and complementary therapeutic strategies. Electroacupuncture (EA), a modern extension of traditional acupuncture, has garnered attention for its potential neuroprotective effects in ischemic stroke rehabilitation. This meta-analysis and systematic review aim to synthesize current experimental evidence on the efficacy of EA in ischemic stroke models, focusing on neurological outcomes, infarct volumes, and underlying molecular mechanisms.

Design, Synthesis, and Biological Evaluation of Novel Fms-Like Tyrosine Kinase 3/VEGFR2/Histone Deacetylase Inhibitors for the Treatment of Acute Myeloid Leukemia.

The concurrent targeting of Fms-like tyrosine kinase 3 (FLT3)/VEGFR2/Histone deacetylase (HDAC) represents a novel and promising therapeutic strategy for acute myeloid leukemia. In this work, we hybridized essential pharmacophores from sorafenib and SAHA (vorinostat) and then conducted structure-activity relationship studies to identify two lead compounds and that potently inhibit FLT3, VEGFR2, and HDAC in a nanomolar range. In cell evaluation, compounds and exhibited potent proliferative activities against a panel of leukemia cells including MV4-11 and MOLM-13.

Development and validation of a prediction model for estimating patency rates after treatment of haemodialysis access dysfunction.

Objective: This study aims to develop a risk assessment model for predicting haemodialysis access dysfunction and to construct a nomogram.

Method: The clinical data of patients with haemodialysis access dysfunction treated at our hospital from October 2020 to January 2024 were retrospectively analysed. The least absolute shrinkage and selection operator regression method was used to filter variables and select predictors, while Cox regression was applied to filter variables and construct a nomogram.

Association Between Triglyceride-Glucose Index and Carotid Plaque Stability in Different Glycemic Status: A Single-Center Retrospective Study.

Background: The triglyceride-glucose (TyG) index has been proposed as a reliable marker of insulin resistance. However, its value in patients with carotid plaque stability remains unclear. This study investigated the association between the TyG index and unstable carotid plaque.

Hyperbaric oxygen therapy for paroxysmal sympathetic hyperactivity syndrome after brain injury: a multicenter, retrospective cohort study.

Hyperbaric oxygen has been used to treat many diseases. However, there are few reports on hyperbaric oxygen treatment for paroxysmal sympathetic hyperactivity at home and abroad, and the clinical experience is very limited. To understand the efficacy of hyperbaric oxygen treatment for paroxysmal sympathetic hyperactivity after brain injury, this retrospective study was conducted in the adult intensive care units of five medical centers in central China.

Development and validation of a risk prediction model for gestational diabetes mellitus in women of advanced maternal age during the first trimester.

With the global rise in advanced maternal age (AMA) pregnancies, the risk of gestational diabetes mellitus (GDM) increases. However, few GDM prediction models are tailored for AMA women. This study aims to develop a practical risk prediction model for GDM in AMA women.

Tailoring the Porous Structure of Carbon for Enhanced Oxidative Cleavage and Esterification of C(CO)-C Bonds.

The cleavage and functionalization of carbon-carbon bonds are crucial for the reconstruction and upgrading of organic matrices, particularly in the valorization of biomass, plastics, and fossil resources. However, the inherent kinetic inertness and thermodynamic stability of C-C σ bonds make this process challenging. Herein, we fabricated a glucose-derived defect-rich hierarchical porous carbon as a heterogeneous catalyst for the oxidative cleavage and esterification of C(CO)-C bonds.

RNA-seq and Ribosome Profiling Reveal the Translational Landscape of Rice in Response to Rice Stripe Virus Infection.

Rice is a crucial staple food for over half the global population, and viral infections pose significant threats to rice yields. This study focuses on the Rice Stripe Virus (RSV), which is known to drastically reduce rice productivity. We employed RNA-seq and ribosome profiling to analyze the transcriptional and translational responses of RSV-infected rice seedlings.

The Astragalus Membranaceus Herb Attenuates Leukemia by Inhibiting the FLI1 Oncogene and Enhancing Anti-Tumor Immunity.

Astragalus membranaceus (AM) herb is a component of traditional Chinese medicine used to treat various cancers. Herein, we demonstrate a strong anti-leukemic effect of AM injected (Ai) into the mouse model of erythroleukemia induced by Friend virus. Chemical analysis combined with mass spectrometry of AM/Ai identified the compounds Betulinic acid, Kaempferol, Hederagenin, and formononetin, all major mediators of leukemia inhibition in culture and in vivo.

Review on synthesis of lactic acid and lactates from biomass derived carbohydrates via chemocatalysis routes.

The utilization of renewable lignocellulosic biomass resources is a promising solution to deal with the deficit of fossil resources and the associated environmental concerns. Among diverse biomass-derived products, lactic acid (LA) stands out as one of the most successful commodities and also a platform to connect raw biomass feedstocks with value-added chemicals and degradable polymers. Herein, we critically review the recent advances in the design and development of base, acid, and multifunctional catalytic systems for the conversion of different carbohydrates to LA and alkyl lactates via chemical routes.

Effects of polysaccharides on the structure, functionality, emulsion stability and rheological properties of soybean meal hydrolysate-proanthocyanidin complexes.

In this study, the structure, functionality, physicochemical property, emulsion storage stability, and rheological properties of soybean meal hydrolysate-proanthocyanidin (SMH-PC) conjugates in ternary complex with glucan, sodium alginate, or soybean polysaccharides were investigated. Following complexing, the proteins unfolded and their disordered structures positively promoted the emulsifying properties of ternary complexes. The SMH-PC-glucan complex showed the best antioxidant activity and the highest emulsifying activity index (94.

Generation of an induced pluripotent stem cell (iPSC) line (INNDSUi007-A) from a patient with Kennedy disease.

Abnormal trinucleotide CAG repeat expansions in exon 1 of the Androgen Receptor (AR) gene has been identified as the cause of Kennedy disease (KD). We generated and characterized a human induced pluripotent stem cell (iPSC) line from peripheral blood mononuclear cells (PBMC) of a patient with genetically confirmed KD. The pluripotency of these iPSCs was verified by the expression of several pluripotency markers at both RNA and protein levels, as well as their capability to differentiate into all three germ layers.

Improved Point-of-Care Mass Spectrometry Analysis with Thin-Layer Chromatography-Based Two-Dimensional Separation and Spray Ionization.

Point-of-care testing (POCT) involves administering rapid on-site analysis to provide fast biochemical testing results. POCT reduces delays in clinical decision-making and eliminates the need to transport and prepare clinical samples for immediate diagnosis or clinical intervention by healthcare professionals. Herein, a novel methodology integrating thin-layer chromatography-based two-dimensional separation with miniature mass spectrometry was developed for rapid on-site clinical analysis.

Non-metallic surface-modified X-Cu (XÂ =Â F, Cl, Br) metal catalysts for all-pH hydrogen evolution reaction with high performance.

Producing hydrogen through water electrolysis represents a clean and sustainable solution that is crucial in addressing the energy crisis. Nonetheless, the slow process of water electrolysis leads to large different kinetics for hydrogen evolution reaction (HER) under different pH solutions. Here, we designed surface modified metallic X-Cu catalysts (X = F, Cl, Br) with different non-metallic elements on nickel foam (NF) using an electrochemical deposition method, which realizes high performance for all-pH range.

Higher Intraocular Levels of Inflammatory Factors are Related to Retinal Vascular and Neurodegeneration in Myopic Retinopathy.

Purpose: In this study, we aimed to investigate the relationship between the intraocular levels of inflammatory factors and myopia-related retinal vascular and neuronal degeneration.

Patients And Methods: One hundred and forty-seven patients with Implantable Collamer Lens (ICL) implantation were enrolled and all participants received comprehensive ophthalmic examination. About 100~150 ul of aqueous humor was collected immediately before ICL surgery.

Insulin resistance, vulnerable plaque and stroke risk in patients with carotid artery stenosis.

Insulin resistance (IR) is linked to both the vulnerable plaque and the stroke risk. However, the precise extent of this correlation and its impact on stroke risk in carotid artery stenosis patients remain unclear. Therefore, this study aims to investigate the relationship between vulnerable plaque and IR and stroke risk and the mediating role of vulnerable plaque in patients with carotid artery stenosis.

Mitochondria-Associated Gene SLC25A32 as a Novel Prognostic and Immunotherapy Biomarker: From Pan-Cancer Multiomics Analysis to Breast Cancer Validation.

Background: Mutations in SLC25A32 in humans cause late-onset exercise intolerance, which is associated with various neurological and metabolic diseases. However, its specific mechanism of action in tumour development is poorly understood owing to the lack of multiomics integrated analysis of SLC25A32 in pan-cancer.

Methods: We used various analytical tools to comprehensively investigate the transcription, protein level, and promoter methylation of SLC25A32.

The analgesic effectiveness of auriculotherapy for acute postoperative pain: A systematic review and meta-analysis.

Background: Postoperative pain is a significant yet inadequately managed complication following surgery, and auriculotherapy to alleviate acute postoperative pain (APP) and reduce the use of opioids remains controversial.

Methods: We searched the MEDLINE, Web of Science, Embase, Cochrane Library, CINAHL Complete, and ClinicalTrials.gov from inception to January 23, 2024 for all randomized controlled trials (RCTs) of auriculotherapy in the treatment of APP.