Differences in the pathological, transcriptomic, and prognostic implications of lymphoid structures between primary and metastatic cutaneous melanomas.

Background: While the prognostic role of tertiary lymphoid structures (TLS) has been well studied in solid cancers, the prevalence and impact of immature precursor lymphoid structures known as lymphoid aggregates (LA) remain unresolved in relation to the disease process. In this study, we examined characteristics and the prognostic utility of LA and TLS status in histological samples from patients with melanoma.

Methods: We assessed The Cancer Genomic Atlas-skin cutaneous melanoma digital slides and melanoma specimens from the University of Pittsburgh for the presence of LA and TLS using H&E staining, multiplex immunofluorescence (mIF) and transcriptomic analyses.

Biodegradation of commercial polyester polyurethane by a soil-borne bacterium Bacillus velezensis MB01B: Efficiency, degradation pathway, and in-situ remediation in landfill soil.

Polyurethane (PU), a widely used and durable plastic, persists in the environment, resulting in significant waste management challenges. Therefore, developing eco-friendly degradation technologies, such as screening for efficient biodegrading microorganism strains, is urgently needed to address this issue. Bacillus velezensis MB01B, an efficient polyester PU-degrading bacterium, was isolated from landfill soil and demonstrated the ability to degrade 91.

Porous and Homogeneous Nanoheterojunction-Accumulating PdO@ZnO Structure for Exhaled Breath Ammonia Sensing.

The functional gas sensor device plays a pivotal role in intelligent medical treatment, among which metal oxide semiconductors are widely studied because of their inexpensiveness and ease of fabrication. However, the metal oxide sensors present a significant challenge in detecting NH at ppm levels within complex exhaled gases. Herein, the ZnO/PdO- series were prepared by in situ loading palladium particles and calcining using nano-ZIF-8 as a precursor, which not only provided more transport path for ammonia adsorption but also achieved homogeneous nanoheterojunction accumulation structure.

Nanobiohybrid Extracellular Vesicle Nanoreactor with Improving Metabolical Activity for Biocatalytic Therapy.

Extracellular vesicles (EVs) hosting enzymatic activities that function as independent metabolic units are attractive natural biocatalytic platforms. However, directly using these metabolically active nanoreactors for effective biocatalytic applications remains challenging, mainly due to their constrained catalytic capabilities. Here, we construct an EV-templated nanobiohybrid system by engineering an EV surface with a photoresponsive zeolitic imidazolate framework (ZIF).

Model of superior semicircular canal dehiscence: asymmetrical vestibular dysfunction induces reversible balance impairment.

Background: Superior semicircular canal dehiscence (SSCD) is a vestibular-cochlear disorder in humans in which a pathological third mobile window of the otic capsule creates changes to the flow of sound pressure energy through the perilymph/endolymph. The primary symptoms include sound-induced dizziness/vertigo, inner ear conductive hearing loss, autophony, headaches, and visual problems. We have developed an animal model of this human condition in the Mongolian Gerbil that uses surgically created SSCD to induce the condition.

An integrated microbiome- and metabolome-genome-wide association study reveals the role of heritable ruminal microbial carbohydrate metabolism in lactation performance in Holstein dairy cows.

Background: Despite the growing number of studies investigating the connection between host genetics and the rumen microbiota, there remains a dearth of systematic research exploring the composition, function, and metabolic traits of highly heritable rumen microbiota influenced by host genetics. Furthermore, the impact of these highly heritable subsets on lactation performance in cows remains unknown. To address this gap, we collected and analyzed whole-genome resequencing data, rumen metagenomes, rumen metabolomes and short-chain fatty acids (SCFAs) content, and lactation performance phenotypes from a cohort of 304 dairy cows.

High speed innovations in photoacoustic microscopy.

Photoacoustic microscopy (PAM) is a key implementation of photoacoustic imaging (PAI). PAM merges rich optical contrast with deep acoustic detection, allowing for broad biomedical research and diverse clinical applications. Recent advancements in PAM technology have dramatically improved its imaging speed, enabling real-time observation of dynamic biological processes in vivo and motion-sensitive targets in situ, such as brain activities and placental development.

Circulating Galectin-3: A Prognostic Biomarker in Hepatocellular Carcinoma.

Introduction: Galectin-3 plays critical roles in the adhesion, proliferation, and differentiation of tumor cells. Recent data have suggested that galectin-3 plays a role in the development of hepatocellular carcinoma (HCC); however, its prognostic value has not been validated. The aim of our study was to evaluate the clinical and prognostic value of galectin-3 in patients with HCC.

Radio-pathomic estimates of cellular growth kinetics predict survival in recurrent glioblastoma.

A radio-pathomic machine learning (ML) model has been developed to estimate tumor cell density, cytoplasm density (Cyt) and extracellular fluid density (ECF) from multimodal MR images and autopsy pathology. In this multicenter study, we implemented this model to test its ability to predict survival in patients with recurrent glioblastoma (rGBM) treated with chemotherapy. Pre- and post-contrast T-weighted, FLAIR and ADC images were used to generate radio-pathomic maps for 51 patients with longitudinal pre- and post-treatment scans.

Biomarkers of rheumatoid arthritis-associated interstitial lung disease: a systematic review and meta-analysis.

Background: Interstitial Lung Disease (ILD) represents the most common extra-articular manifestation of Rheumatoid Arthritis (RA) and is a major cause of mortality. This study aims to identify and evaluate biomarkers associated with Rheumatoid Arthritis-Associated Interstitial Lung Disease (RA-ILD).

Methods: We searched PubMed, Cochrane Library, EMBASE, and Web of Science databases for studies related to biomarkers of RA-ILD up until October 7, 2023.

Correlation analysis between urinary crystals and upper urinary calculi.

Objective: This study aimed to analyze the correlation between urinary crystals and urinary calculi.

Methods: Clinical data, including urinary crystal types, were collected from 237 patients with urinary calculi. The detection rate of urine crystals and their correlation with stone composition were analyzed.

Prepatellar bursa mucosa: An unreported postoperative complication of patellar fracture.

Background: Patellar fracture is a common injury caused by overstrain of the extensor mechanism or a direct trauma to the knee. The most common complications after patellar fracture are nonunion, infection, post-traumatic arthritis, joint fibrosis, symptomatic hardware, and extensor mechanism failure. These are attributed to the post-fracture flexion and extension movements, the primary damage to the articular cartilage, and the thin soft tissue coverage over the knee.

The Development, Essence and Perspective of Nitrogen Reduction to Ammonia.

Ammonia plays a pivotal role in agriculture and meanwhile holds promising potential as an energy vector for the hydrogen economy, where the nitrogen reduction to ammonia is a critical pathway for achieving sustainable development. Over the past hundred years, ammonia synthesis has undergone several breakthrough developments from Haber-Bosch process to photo/electro-catalysis and Li-mediated strategy, but still faces the challenges of low yield rate, selectivity and efficiency. Therefore, there is a pressing demand to develop efficient and green ammonia synthesis from nitrogen.

Spatiotemporal modeling of molecular holograms.

Quantifying spatiotemporal dynamics during embryogenesis is crucial for understanding congenital diseases. We developed Spateo (https://github.com/aristoteleo/spateo-release), a 3D spatiotemporal modeling framework, and applied it to a 3D mouse embryogenesis atlas at E9.

Three-Dimensional Semiconductor Network as Regulators of Energy Metabolism Drives Angiogenesis in Bone Regeneration.

Insufficient vascularization is a primary cause of bone implantation failure. The management of energy metabolism is crucial for the achievement of vascularized osseointegration. In light of the bone semiconductor property and the electric property of semiconductor heterojunctions, a three-dimensional semiconductor heterojunction network (3D-NTBH) implant has been devised with the objective of regulating cellular energy metabolism, thereby driving angiogenesis for bone regeneration.

Deep tissue photoacoustic imaging with light and sound.

Photoacoustic computed tomography (PACT) can harvest diffusive photons to image the optical absorption contrast of molecules in a scattering medium, with ultrasonically-defined spatial resolution. PACT has been extensively used in preclinical research for imaging functional and molecular information in various animal models, with recent clinical translations. In this review, we aim to highlight the recent technical breakthroughs in PACT and the emerging preclinical and clinical applications in deep tissue imaging.

Single-cell transcriptomic and cross-species comparison analyses reveal distinct molecular changes of porcine testes during puberty.

The pig is an important model for studying human diseases and is also a significant livestock species, yet its testicular development remains underexplored. Here, we employ single-cell RNA sequencing to characterize the transcriptomic landscapes across multiple developmental stages of Bama pig testes from fetal stage through infancy, puberty to adulthood, and made comparisons with those of humans and mice. We reveal an exceptionally early onset of porcine meiosis shortly after birth, and identify a distinct subtype of porcine spermatogonia resembling transcriptome state 0 spermatogonial stem cells identified in humans, which were previously thought to be primate specific.

Research on prediction of surrounding rock deformation and optimization of construction parameters of high ground stress tunnel based on WOA-LSTM.

To accurately understand the deformation behavior of surrounding rock in the jiugongshan No.2 high ground stress tunnel and optimize construction parameters for improved efficiency, on-site monitoring was used to gather data on rock deformation and initial support contact pressure. A WOA-LSTM regression prediction model was proposed, and excavation advance depth was optimized using numerical simulation.

Effect of Laser on the Interface and Thermal Conductivity of Metallized Diamond/Cu Composite Coatings Deposited by Supersonic Laser Deposition.

To achieve the rapid heat dissipation of components in the industrial field, the heat dissipation coating is prepared on the surface, which is conducive to improving the service life of the parts and greatly reducing the industrial costs. In this paper, metallized diamond/Cu composite coatings were fabricated on 1060Al substrate by supersonic laser deposition. The composite coatings were prepared at a nitrogen pressure of 3.