Atp1a2 and Kcnj9 Are Candidate Genes Underlying Sensitivity to Oxycodone-Induced Locomotor Activation and Withdrawal-Induced Anxiety-Like Behaviors in C57BL/6 Substrains.

Opioid use disorder is heritable, yet its genetic etiology is largely unknown. C57BL/6J and C57BL/6NJ mouse substrains exhibit phenotypic diversity in the context of limited genetic diversity which together can facilitate genetic discovery. Here, we found C57BL/6NJ mice were less sensitive to oxycodone (OXY)-induced locomotor activation versus C57BL/6J mice in a conditioned place preference paradigm.

The Interplay Between Stigma and Asian Cultural Values in People with Parkinson's Disease and Their Caregivers.

Parkinson's Disease (PD), the second most prevalent neurodegenerative disorder globally, often carries stigma for those affected; however, stigma's impact on PD caregivers is not well understood. This study investigates PD stigma and its interplay with cultural values, as well as explores the role of depressive symptomatology in shaping help-seeking attitudes among individuals with PD and the provision of support by PD caregivers. Using cross-sectional data collected from people living with PD and their caregivers, this study examines how adherence to Asian values influences the link between self-stigma and help-seeking tendencies in PD patients, as well as how affiliate stigma is linked to caregivers' willingness to help.

Study on pH-Responsive Delayed, Cross-Linking and Weighted Fracturing Fluid.

Hydraulic fracturing of deep, high-temperature reservoirs poses challenges due to elevated temperatures and high fracture pressures. Conventional polymer fracturing fluid (QCL) has high viscosity upon adding cross-linking agents and significantly increases wellbore friction. This paper examines a polymer fracturing fluid with pH response and low friction.

How socioeconomic and structural barriers influence dental care among transgender people.

Background: While recent US policies restrict access to healthcare and resulting health disparities among the transgender community, little is known about oral health access and utilization among this population. This study assessed self-reported access to dental care among transgender adults living in the United States.

Method: The study sample included 1,284,526 observations representing a weighted population of 290,000,163 from Behavioral Risk Factor and Surveillance Survey (BRFSS) datasets.

Comprehensive effects of sediment dredging on environmental risk and bioavailability of heavy metals from the sediment of Lake Taihu, China.

The comprehensive effects of environmental dredging on heavy metals (HM) are still uncertain. This study comprehensively evaluates the long-term effects of dredging on the environmental risk and bioavailability of HM (Cu, Ni, Zn, Pb, Cd, Cr, and As) in Lake Taihu, China, by comparing simulated dredged treated (D) and undredged (UD) sediment cores under in-situ conditions for one year. Threshold effect level (TEL), geological accumulation index (I), potential ecological risk index (RI), and ratios of secondary phase and primary phase (RSP) methods were used to assess the environmental risk of sediment HM; and the diffusive gradient in thin-films (DGT) technique was applied to assess the bioavailability of sediment HM.

Tooth Root Development and Homeostasis during Eruptive and Post-eruptive Movement.

Tooth eruption is the process whereby the developing tooth moves to its functional position in the occlusal plane and it occurs concomitantly with formation of the tooth root, which is a critical component of the tooth anchored to surrounding alveolar bone through the periodontal ligament. Post-eruptive tooth movement ensues that once occlusion is achieved, the teeth maintain their alignment within the alveolar bone to facilitate proper bite function through periodontium remodelling. Tooth overeruption presents a clinically significant issue, yet the precise mechanisms by which alterations in occlusal forces are translated into periodontal remodelling remain largely unexplored.

Interpretable multi-modal artificial intelligence model for predicting gastric cancer response to neoadjuvant chemotherapy.

Neoadjuvant chemotherapy assessment is imperative for prognostication and clinical management of locally advanced gastric cancer. We propose an incremental supervised contrastive learning model (iSCLM), an interpretable artificial intelligence framework integrating pretreatment CT scans and H&E-stained biopsy images, for improved decision-making regarding neoadjuvant chemotherapy. We have constructed and tested iSCLM using retrospective data from 2,387 patients across 10 medical centers and evaluated its discriminative ability in a prospective cohort (132 patients; ChiCTR2300068917).

Programmed BRD9 Degradation and Hedgehog Signaling Activation via Silk-Based Core-Shell Microneedles Promote Diabetic Wound Healing.

Wound healing impairment in diabetes mellitus is associated with an excessive inflammatory response and defective regeneration capability with suppressed Hedgehog (Hh) signaling. The bromodomain protein BRD9, a subunit of the non-canonical BAF chromatin-remodeling complex, is critical for macrophage inflammatory response. However, whether the epigenetic drug BRD9 degrader can attenuate the sustained inflammatory state of wounds in diabetes remains unclear.

Making waves: Harnessing stormwater for resilient water supply - A blueprint for vulnerable continental coasts and oceanic islands.

At the forefront of climate change and natural disasters, small islands like Puerto Rico confront immense challenges in sustaining an adaptive water supply. The challenges are further exacerbated when the islands' constraints, such as geographic isolation, limited resources, aging infrastructure, constrained capacity, and environmental fragility, are compounded. As these issues increasingly impact low-lying continental coasts, home to approximately 40 % of the global population, similar water supply crises have already emerged or are anticipated in these regions.

Topical Protease Inhibitor Increases Tumor-Free and Overall Survival in CD4-Depleted Mouse Model of Anal Cancer.

Patients with immunodeficiencies and older age are at an increased risk of anal cancer. Transgenic mice with established high-grade anal dysplasia were treated topically at the anus with the protease inhibitor saquinavir (SQV) in the setting of CD4+ T-cell depletion to mimic immunodeficiency. To ensure tumor development, specific groups were treated with a topical carcinogen (7,12-Dimethylbenz[a]anthracene (DMBA)).

Microglia synchronizes with the circadian rhythm of the glymphatic system and modulates glymphatic system function.

Microglia, as immune cells in the central nervous system, possess the ability to adapt morphologically and functionally to their environment. Glymphatic system, the principal waste clearance system in the brain, exhibits circadian rhythms. However, the impact of microglia on the glymphatic system function remains unknown.

Ethnic-specific characteristics associated with longitudinal response patterns from prebirth to 12 years: evidence from .

Background: Longitudinal studies can generate valuable scientific knowledge, but can be compromised by systematic attrition. Previous research shows that sociodemographic characteristics (eg, ethnicity, age, educational level, socioeconomic circumstances) are associated with attrition rates. However, little is known about whether these characteristics differ by ethnicity, and how this impacts cohort retention strategies.

STOP-HCV-HCC Program: Privacy-Preserving Innovation for Remote Data Access Analytics at Federally Qualified Health Centers in South Texas.

STOP-HCV-HCC program to screen and treat hepatitis C, vaccinate for hepatitis B, and prevent hepatocellular carcinoma is implementing a cloud-based privacy-preserving platform to overcome electronic health record barriers to reporting, without data transfer, at four federally qualified health centers in South Texas, USA.

Differential toxic effects of nano-titanium dioxide on clams (Meretrix meretrix) with various individuality.

Nano-TiO is inevitably released into aquatic environment with increasing of nanotechnology industries. Study pointed that different individuality showed divergent behavioral and physiological response when facing environmental stress. However, the effects of nano-TiO on tolerance of bivalves with different individualities remain unknown.

Knowledge, attitudes, and practices of orthopedic operating room personnel regarding the use of pneumatic tourniquets.

Introduction: Pneumatic tourniquets (PTs) play a crucial role in hemostasis during orthopedic surgery. This study aimed to investigate the current knowledge, attitudes, and practices (KAP) of orthopedic operating room personnel concerning the use of PTs.

Methods: This cross-sectional study was conducted from Jul.

ARID1A safeguards the canalization of the cell fate decision during osteoclastogenesis.

Chromatin remodeler ARID1A regulates gene transcription by modulating nucleosome positioning and chromatin accessibility. While ARID1A-mediated stage and lineage-restricted gene regulation during cell fate canalization remains unresolved. Using osteoclastogenesis as a model, we show that ARID1A transcriptionally safeguards the osteoclast (OC) fate canalization during proliferation-differentiation switching at single-cell resolution.

The ultrasonic vocalization (USV) syllable profile during neonatal opioid withdrawal and a kappa opioid receptor component to increased USV emissions in female mice.

Rationale: Opioid use during pregnancy can lead to negative infant health outcomes, including neonatal opioid withdrawal syndrome (NOWS). NOWS comprises gastrointestinal, autonomic nervous system, and neurological dysfunction that manifest during spontaneous withdrawal. Variability in NOWS severity necessitates a more individualized treatment approach.

Optimizing acid microemulsions for cleaner gas production: A study on enhanced adsorption characteristics and implications in retardation.

The ultralow interfacial tension between the oil and aqueous phases and the solubilization characteristics in microemulsion systems make them useful for surface cleaning and enhanced oil recovery applications. Microemulsions can form an adsorbed barrier on rock, reducing the acid-rock reaction rate. However, as acid retardation additives, the adsorption patterns of microemulsions are not clearly defined.

An artificial intelligence grading system of apical periodontitis in cone-beam computed tomography data.

Objectives: In order to assist junior doctors in better diagnosing apical periodontitis (AP), an artificial intelligence AP grading system was developed based on deep learning (DL) and its reliability and accuracy were evaluated.

Methods: One hundred and twenty cone-beam computed tomography (CBCT) images were selected to construct a classification dataset with four categories, which were divided by CBCT periapical index (CBCTPAI), including normal periapical tissue, CBCTPAI 1-2, CBCTPAI 3-5, and young permanent teeth. Three classic algorithms (ResNet50/101/152) as well as one self-invented algorithm (PAINet) were compared with each other.

and are candidate genes underlying sensitivity to oxycodone-induced locomotor activation and withdrawal-induced anxiety-like behaviors in C57BL/6 substrains.

Opioid use disorder is heritable, yet its genetic etiology is largely unknown. C57BL/6J and C57BL/6NJ mouse substrains exhibit phenotypic diversity in the context of limited genetic diversity which together can facilitate genetic discovery. Here, we found C57BL/6NJ mice were less sensitive to oxycodone (OXY)-induced locomotor activation versus C57BL/6J mice in a conditioned place preference paradigm.

Heat-inducible CAR-T overcomes adverse mechanical tumor microenvironment in a 3D bioprinted glioblastoma model.

Glioblastoma (GBM) presents a significant therapeutic challenge due to the limited efficacy of existing treatments. Chimeric antigen receptor (CAR) T-cell therapy offers promise, but its potential in solid tumors like GBM is undermined by the physical barrier posed by the extracellular matrix (ECM). To address the inadequacies of traditional 2D cell culture, animal models, and Matrigel-based 3D culture in mimicking the mechanical characteristics of tumor tissues, we employed biomaterials and digital light processing-based 3D bioprinting to fabricate biomimetic tumor models with finely tunable ECM stiffness independent of ECM composition.