Single-cell sequencing uncovers the mechanistic role of DAPK1 in glioma and its diagnostic and prognostic implications.

Background: We conducted an investigation into the characteristics of single-cell differentiation data in gliomas, with a focus on developing DAPK1-based prognostic markers to predict patient outcomes. Dysregulated expression of DAPK1 has been associated with the invasive behavior of various malignancies, including gliomas. However, the precise role and underlying mechanisms of DAPK1 in gliomas remain inadequately understood.

HS Regulates the Phenotypic Transformation of Astrocytes Following Cerebral Ischemia/Reperfusion via Inhibiting the RhoA/ROCK Pathway.

The role of hydrogen sulfide (HS) on the phenotypic change of astrocytes following cerebral ischemia/reperfusion (I/R) in mice was investigated in present study. We tested the expression of glial fibrillary acidic protein (GFAP), A2 phenotype marker S100a10, and A1 phenotype marker C3 protein and assessed the change of BrdU/GFAP-positive cells, GFAP/C3-positive cells, and GFAP/S100a10-positive cells in mice hippocampal tissues to evaluate the change of astrocyte phenotypes following cerebral I/R. The role of HS on the phenotypic change of astrocytes following cerebral I/R in mice was investigated by using HS synthase cystathionine-γ-lyase (CSE) knockout mice (KO).

Total flavones of Rhododendron induce the transformation of A1/A2 astrocytes via promoting the release of CBS-produced HS.

Background: We previously found that total flavones of Rhododendron (TFR) protected against the cerebral ischemia/reperfusion (I/R) injury. But the detailed mechanism is not clear. Recent research revealed that reactive astrocytes were divided into A1 and A2 phenotypes for their morphological and functional remodeling and neurotoxic- vs-neuroprotective effect on the injury of the central nervous system (CNS).

CSE/HS ameliorates colitis in mice protection of enteric glial cells and inhibition of the RhoA/ROCK pathway.

The enteric glial cells (EGCs) participate in the homeostasis of the gastrointestinal tract, and RhoA/ROCK signaling pathway plays a vital role in colonic tight junctions. Hydrogen sulfide (HS) has been reported to alleviate colitis. However, the effect and mechanism of endogenous HS on colitis remain unclear.

Rutin Inhibits the Progression of Osteoarthritis Through -Mediated Signaling.

Osteoarthritis (OA) is a chronic joint disease characterized by the deterioration of cartilage and subchondral bone in the joints. Currently, there is no complete cure for OA, only treatments designed to temporarily relieve pain and improve function. Compared with the high cost of surgical treatment, medical treatment of OA is more acceptable and cost-effective.

A julolidine-chalcone-based fluorescent probe for detection of Al in real water sample and cell imaging.

A fluorescent probe 1 based on julolidine-chalcone derivative, which can specifically recognize aluminum ion with high selectivity and anti-interference, was developed. Probe 1 has good fluorescence stability and can detect Al with turn-on fluorescence in a wide pH range of 4.0-9.

Synergy of Oxygen Vacancies and Acid Sites on N-Doped WO Nanobelts for Efficient C-C Coupling Synthesis of Benzoin Isopropyl Ether.

The surface property of a photocatalyst, including surface acid sites and oxygen vacancies, plays a pivotal role in photocatalytic organic synthesis reactions. Benzoin isopropyl ether (BIE) is usually produced via polycondensation of benzaldehyde and catalyzed with highly toxic . Here, we report a green photocatalytic approach for the selective synthesis of BIE over WO driven by a green-light-emitting diode.

Hydrogen sulphide protects mice against the mutual aggravation of cerebral ischaemia/reperfusion injury and colitis.

This study was undertaken to determine whether ischaemia/reperfusion (I/R)-induced brain injury and dextran sulfate sodium (DSS)-induced colitis in mice are related. A cerebral I/R model of mice was established by blocking the bilateral common carotid arteries; 3% DSS in drinking water was administered to mice for 7 days to induce colitis; mice with cerebral I/R and colitis were administered DSS for 7 days from the third day onwards after acute cerebral I/R. Brain damage and intestinal inflammation were also tested.

CSE-Derived HS Inhibits Reactive Astrocytes Proliferation and Promotes Neural Functional Recovery after Cerebral Ischemia/Reperfusion Injury in Mice Via Inhibition of RhoA/ROCK Pathway.

The effect of cystathionine-γ-lyase (CSE)-derived hydrogen sulfide (HS) on the reactive proliferation of astrocytes and neural functional recovery over 30 d after acute cerebral ischemia and reperfusion (I/R) was determined by applying wild-type (WT) and CSE knockout (KO) mice. The changes of glial fibrillary acidic protein (GFAP) expression in hippocampal tissues was tested. Besides, we assessed the changes of mice spatial learning memory ability, neuronal damage, RhoA, Rho kinase 2 (ROCK), and myelin basic protein (MBP) expressions in hippocampal tissues.