Development of a High-Resolution Melting Method for the Detection of Clarithromycin-Resistant in the Gastric Microbiome.

The issue of () resistance to clarithromycin (CLR) has consistently posed challenges for clinical treatment. Hence, a rapid susceptibility testing (AST) method urgently needs to be developed. In the present study, 35 isolates of were isolated from 203 gastritis patients of the Guangzhou cohort, and the antimicrobial resistance phenotypes were associated with their genomes to analyze the relevant mutations.

The effectiveness of online breastfeeding education and support program on mothers of preterm infants: A quasi-experimental study.

Objective: To develop a WeChat-mini-program-based Online breastfeeding education and support program and explore its impacts on promoting mothers of preterm infants' breastfeeding knowledge, attitudes, self-efficacy, and rates.

Design, Setting, And Participants: This was a quasi-experimentation with two-group comparisons. The eligible preterm mother-infant pairs were recruited from the NICU within seven days postpartum.

A serum LncRNA signature for predicting prognosis of triple-negative breast cancer.

Background: Breast cancer is the leading causes of cancer-associated mortality among women, and triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer. Long non-coding RNAs (LncRNAs) have recently been studied to predict the prognosis of various cancers, but whether it is an effective marker in TNBC is inconclusive.

Methods: We used RNA-sequencing analysis to identify differentially expressed exosomal LncRNAs, and qRT-PCR assay was performed to verify dysregulated LncRNAs in multicenter validation cohorts.

Exploring Effect of Postdischarge Developmental Support Program on Preterm Infant Neurodevelopment and BDNF Gene DNA Methylation.

Background: Although developmental supportive care is an effective approach to improve the long-term psychomotor and/or neurobehavioral function of preterm infants, very limited studies have focused on the impact of after-discharge developmental support. The underlying epigenetic changes are unclear.

Purpose: This study aimed to explore the preliminary effect of an evidence-based Postdischarge Developmental Support Program (PDSP) on preterm infant neurodevelopment and underlying epigenetic changes, including brain-derived neurotrophic factor (BDNF) gene-related DNA methylation and expression.

Synergism between chemisorption and unique electron transfer pathway in S-scheme AgI/g-CN heterojunction for improving the photocatalytic H evolution.

AgI/g-CN S-scheme heterojunction with a unique electron transfer pathway was developed as a catalyst for H evolution. We discussed the behavior of chemisorption and photoexcited charge carriers in photocatalytic reduction on the S-scheme AgI/g-CN heterojunction. It was demonstrated that the path of charge transfer mediated by S-scheme AgI/g-CN heterojunction was favorable for the improvement of electron utilization in photocatalysis.

Transcranial ultrasound diagnostic value of hemodynamic cerebral changes in preterm infants for early-onset sepsis.

Background: Due to the limitation of blood culture diagnosis, this study sought to evaluate the cerebral hemodynamic changes by Doppler ultrasound for timely and objective diagnosis techniques in preterm infants with early onset-neonatal sepsis.

Methods: In this retrospective study, 86 preterm infants treated at the Department of Neonatology, Renmin Hospital of Wuhan University from January 1, 2019 to March 31, 2021, were divided into the following 2 groups: (I) the early onset neonatal sepsis (EONS) group (G1, n=41); (II) the normal control group (G2, n=45). The cerebral hemodynamic changes were examined by transcranial ultrasound.

Exosomal LncRNA RP5-977B1 as a novel minimally invasive biomarker for diagnosis and prognosis in non-small cell lung cancer.

Background: Lung cancer is the leading cause of cancer-related deaths in the world. Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancer cases. For lack of conveniently sensitive and specific biomarkers, the majority of patients are in the late stage at initial diagnosis.

Microbiologic risk factors of recurrent choledocholithiasis post-endoscopic sphincterotomy.

Background: Choledocholithiasis is a severe disorder that affects a significant portion of the world's population. Treatment using endoscopic sphincterotomy (EST) has become widespread; however, recurrence post-EST is relatively common. The bile microbiome has a profound influence on the recurrence of choledocholithiasis in patients after EST; however, the key pathogens and their functions in the biliary tract remain unclear.

Determination of Antiviral Mechanism of Centenarian Gut-Derived Against Norovirus.

Although noroviruses are the causative agents of most non-bacterial foodborne disease outbreaks, effective antivirals are currently unavailable. Certain probiotic strains have been reported as active antivirals for norovirus infections, but their mechanisms have not been fully elucidated. Herein, we examined the antiviral potential of 122 lactic acid bacteria isolates against murine norovirus (MNV), a human norovirus surrogate.

Exploration of the molecular mechanisms underlying the antibiotic resistance of Helicobacter pylori: A whole-genome sequencing-based study in Southern China.

Background: Although antimicrobial resistance (AMR) in Helicobacter pylori is a global threat to human health and the underlying molecular mechanisms have been explored previously, only a few of them are fully elucidated.

Materials And Methods: In the present study, we isolated 54 Helicobacter pylori strains from Southern China and assessed their susceptibility to five antibiotics using the agar dilution assay. Whole-genome sequencing was performed to screen the AMR genotypes of the Helicobacter pylori isolates.

Imbalanced Dermic Microbiome Aggravates Inflammation in Toenail Paronychia.

The commensal microbiome influences skin immunity, but its function in toenail health remains unclear. Paronychia is one of the most common inflammatory toenail diseases, but antibiotic treatment is seldom effective in clinical cases. In this study, we performed sequencing to investigate the characteristics of microbes associated with paronychia in order to identify the key microorganisms involved in inflammation.

Acupuncture: A therapeutic approach against acne.

Acne is a common but chronic skin disease that influence large population especially juvenile. Acne can continue, begin, or grow into severe form in adult age, affecting face, back, and chest. It may be a serious issue if not cared or treated timely.

Distribution of complete blood count constituents in gestational diabetes mellitus.

Background Trial Design: The incidence rate of gestational diabetes is high. In the long run, it harms the health of both the mother and child. In order to understand the distribution of hematological cells with gestational diabetes mellitus (GDM), a longitudinal cohort study was conducted from 2012 to 2018.

Clinical values of common biomarkers for efficacy monitoring of antibiotics in early-onset neonatal sepsis.

Background: To investigate the clinical values of the common biomarkers including blood routine (B-Rt), C-reactive protein (CRP), serum amyloid A (SAA) and procalcitonin (PCT) for efficacy monitoring of antibiotics in early-onset neonatal sepsis (EONS).

Methods: The clinical and laboratory data of 78 neonates with confirmed EONS in the neonatal intensive care unit (NICU) of our center from July 1, 2019 to June 30, 2020 were retrospectively analyzed. All the subjects were treated with cefotiam (50 mg/kg q12h) and augmentin (30 mg/kg q12h) within 12 hours after birth.

Plasmon Sensitized Heterojunction 2D Ultrathin Ag/AgI-δ-BiO for Enhanced Photocatalytic Nitrogen Fixation.

A novel 2D ultrathin Ag/AgI-δ-BiO photocatalyst was constructed by a facile hydrothermal and in situ photodeposition method, which presented a uniform nanosheet structure with an average height of 6 nm. Its composition, morphology and light-harvesting properties were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-vis spectrophotometer (UV-vis) and photoluminescence (PL) measurements in detail. The Ag/AgI-δ-BiO nanocomposites showed an excellent photocatalytic nitrogen fixation performance of 420 μmol L g h in water without any sacrificial agent.

Multilayer ultrathin Ag-δ-BiO with ultrafast charge transformation for enhanced photocatalytic nitrogen fixation.

Photocatalytic nitrogen fixation provides an attractive approach to produce reactive nitrogen compounds at benign conditions. Herein, Ag decorated δ-BiO photocatalyst, which has the features of multilayer ultrathin structure, suitable absorption edge and many exposed surface sites, is prepared by a hydrothermal and photoreduction process. Under visible light illustration, the obtained Ag-δ-BiO photocatalyst exhibits efficient photocatalytic activity for NH generation at room temperature and atmospheric pressure in aqueous solution.

Polyphenol content of plasma and litter after the oral administration of green tea and tea polyphenols in chickens.

Metabolic profiles of broiler chickens were examined after the ingestion of green tea, tea polyphenols, and (-)-epigallocatechin-3-gallate (EGCG). Solid-phase extraction of serum and litters yielded free catechins and their metabolites, which were then identified and quantified by liquid chromatography-tandem mass spectrometry. In plasma samples, (-)-gallocatechin, (+)-catechin, and EGCG were detected in the green tea group; pyrogallol acid, (epi)catechin-O-sulfate, 4'-O-methyl-(epi)gallocatechin-O-glucuronide, and (epi)catechin-3'-O-glucuronide were detected in the tea polyphenols group; and EGCG, (-)-gallocatechin gallate (GCG), and 4'-O-methyl-(epi)gallocatechin-O-glucuronides were detected in the EGCG group.

Photodegradation of new herbicide HW-02 in organic solvents.

HW-02 is a new organophosphates herbicide which is discovered and developed in China. The kinetics and mechanism of HW-02 photodegradation in the organic solvents were studied at 25 degrees C under the irradiation of ultraviolet light. The results showed that photochemical reaction of HW-02 in organic solvents such as n-hexane, methanol, dimethyl benzene and acetone under UV light could be well described by the first kinetic equation, and the photodegradation efficiency decreased with a order of n-hexane > methanol > xylene > acetone.