Management of Severe Dysphonia and Dysphagia Following Lateral Skull Base Surgery.

Objectives: Lateral skull base surgeries pose a risk of injuring the lower cranial nerves, leading to potential postoperative complications such as dysphonia and dysphagia. Conservative treatments have shown limited efficacy in addressing these resultant voice and swallowing dysfunctions, significantly impacting patient quality of life. This study aims to evaluate the safety and effectiveness of a combined surgical approach involving autologous fat injection laryngoplasty (AFIL) and transcervical cricopharyngeal myotomy (TCPM) in patients suffering from severe dysphonia and dysphagia following lateral skull base surgery.

Transoral Endoscopic Excision of Laryngeal Schwannoma.

Objective: Schwannomas originating within the larynx are rare and there is no clear consensus on the diagnostic workup or treatment. This study aims to explore the clinical features of laryngeal schwannoma and evaluate the surgical method of transoral endoscopic excision, especially with coblation.

Methods: Data from patients diagnosed with laryngeal schwannoma who underwent surgery at Peking Union Medical College Hospital from 2017 to 2022 were retrospectively analyzed.

Cross-modal attention network for retinal disease classification based on multi-modal images.

Multi-modal eye disease screening improves diagnostic accuracy by providing lesion information from different sources. However, existing multi-modal automatic diagnosis methods tend to focus on the specificity of modalities and ignore the spatial correlation of images. This paper proposes a novel cross-modal retinal disease diagnosis network (CRD-Net) that digs out the relevant features from modal images aided for multiple retinal disease diagnosis.

Online electrochemistry coupling liquid chromatography-mass spectrometry for rapid investigation on the phase I and phase II simulated metabolic reactions of flavonoids.

In this study, an online electrochemistry coupling high-performance liquid chromatography-mass spectrometry (EC-HPLC-MS) technology has been developed for simulating metabolic reactions and rapid analysis of metabolites of flavone, quercetin, and rutin, which are not only widely present compounds with pharmacological activity in nature, but also have structural similarity and variability. The simulated metabolic processes of the substrates (phase I and phase II metabolism) were implemented on the surface of glassy carbon electrode (GCE) by using different electrochemical methods. After online chromatographic separation, the products were transmitted to a mass spectrometer for detection, in order to speculate relevant reaction pathways and structural information of the reaction product.

Effect of silver sulfadiazine on mature mixed bacterial biofilms on voice prostheses.

Background: Biofilm formation on voice prostheses disrupts the function and limits the lifespan of voice prostheses. There is still no effective clinical strategy for inhibiting or removing these biofilms. Silver sulfadiazine (SSD), as an exogenous antibacterial agent, has been widely used in the prevention and treatment of infection, however, its effect on voice prosthesis biofilms is unknown.

[Efficacy of Activated Autologous Hematopoietic Stem Cell Transplantation in the Treatment of Acute Myeloid Leukemia].

Objective: To compare the efficacy of activated autologous bone marrow and peripheral blood hematopoietic stem cell transplantation (Auto-HSCT) and matched sibling donor allogeneic hematopoietic stem cell transplantation (MSD-HSCT) for the first complete remission of adult acute myeloid leukemia (AML-CR1).

Methods: For 86 adult patients with first complete remission of AML who underwent auto-HSCT (41 cases) and MSD-HSCT (45 cases) in our hospital from June 2012 to June 2020, the patients were treated with modified MAC ［Malflane 160 mg/(m·d), -3 days, Ara-C 2 g/(m·2), -3 days 21∶00, -2 days 9∶00, CTX 60 mg/(kg·d),-3 d, -2 d］, the stem cells were activated by IL-2 (1 000 U/ mL), IFN-α (100 U/ mL) and IFN-γ (100 U/ml). The overall survival (OS), leukemia free survival (LFS), cumulative incidence of recurrence (CIR) and non-recurrence mortality (NRM) of patients with different types of transplantation were compared.

Efficacy and safety of microneedling, fractional CO laser, and cryotherapy combined with 5-aminolevulinic acid photodynamic therapy in the treatment of actinic keratosis: A multicenter prospective randomized controlled study.

Background: Photodynamic therapy (PDT) for actinic keratosis (AK) is limited by the depth of treatment. Microneedling or fractional CO laser can facilitate the penetration of photosensitizer, while cryotherapy can treat deeper tissues but is not suitable for field cancerization.

Objective: To investigate the efficacy of microneedling, fractional CO laser, and cryotherapy in combination with PDT for AK.

Inhibition and Removal of Mature Mixed-Bacteria Biofilms on Voice Prostheses by Sodium Selenite.

Purpose: Biofilms on voice prostheses are important factors shortening their service life. Sodium selenite has been used to prevent and treat various diseases. Whether sodium selenite can inhibit and remove mature biofilms on voice prostheses is still unknown.

Spatial Allocation Method of Evacuation Guiders in Urban Open Public Spaces: A Case Study of Binjiang Green Space in Xuhui District, Shanghai, China.

Evacuation guiders play an important role when emergency events occur in urban open public spaces. Considering the shortcomings of the existing studies, an optimization method based on the Particle Swarm Optimization (PSO) algorithm and gradual covering model for spatial allocation of evacuation guiders in urban open public spaces is proposed. This method considers the impact of obstacles on intervisibility between guiders and evacuees, and the non-linear changing characteristics of the evacuation guiding quality based on the distances between guiders and evacuees to optimize the space allocation of evacuation guiders in urban open public spaces.

In-situ growth of nitrogen-doped carbonized polymer dots on black phosphorus for electrochemical DNA biosensor of Escherichia coli O157: H7.

Sensitive and accurate detection technology for pathogenic bacteria is of great social and economic significance in foodborne disease and food safety. In this paper, a novel portable electrochemical DNA biosensor for the detection of specific DNA sequence of Escherichia coli (E. coli) O157: H7 was constructed.

Clinical study of injectable collagen scaffold with autologous fat cells for repair of severe vocal fold injury.

Cell retention and survival after transplantation remains a major problem for long-term efficiency in therapy of severe vocal fold injury with autologous cells. In this study, injectable collagen scaffold was used to deliver autologous fat cells (AFCs) for repairing of severe vocal fold injury. We found injectable collagen scaffold could enhance the retention and survival of green fluorescent protein (GFP) labeled fat cells in the transplantation sites in rats.

Non-classic splicing mutation in the CPLANE1 (C5orf42) gene cause Joubert syndrome in a fetus with severe craniocerebral dysplasia.

Backgroud: Joubert syndrome is a rare neurodevelopmental disorder characterized by clinical and genetic heterogeneity. The characteristic molar tooth sign, which resulted from cerebellar vermis hypoplasia and midbrain anomalies, is expected to be the key diagnostic feature for this disease. However, it is not easy to make a definite diagnosis in prenatal only based on the imageology due to its clinical heterogeneity.

Post-COVID-19 Epidemic: Allostatic Load among Medical and Nonmedical Workers in China.

Background: As the fight against the COVID-19 epidemic continues, medical workers may have allostatic load.

Objective: During the reopening of society, medical and nonmedical workers were compared in terms of allostatic load.

Methods: An online study was performed; 3,590 Chinese subjects were analyzed.

Effects of Antiacid Therapy on Granuloma after Transoral Type IV-VI Cordectomy in Patients with Early-Stage Glottic Cancer.

Objective: To investigate whether preventive administration of a proton pump inhibitor (PPI) can reduce the occurrence and development of traumatic granuloma (TG) following type IV-VI cordectomy.

Methods: We retrospectively analyzed the status of postoperative granulomas in 37 patients who underwent type IV-VI cordectomy due to glottic cancer and determined whether postoperative administration of a PPI had any impact on granuloma formation and development.

Results: The percentage and number of patients with granuloma in the PPI treatment group (experimental group) at the 1st, 2nd, 3rd, and 6th month following surgery were 81.

COVID-19 pandemic: study on simple, easy, and practical relaxation techniques while wearing medical protective equipment.

Background: No studies have reported on how to relieve distress or relax in medical health workers while wearing medical protective equipment in coronavirus disease 2019 (COVID-19) pandemic. The study aimed to establish which relaxation technique, among six, is the most feasible in first-line medical health workers wearing medical protective equipment.

Methods: This was a two-step study collecting data with online surveys.

Fabrication of ZIF-67@three-dimensional reduced graphene oxide aerogel nanocomposites and their electrochemical applications for rutin detection.

Three-dimensional reduced graphene oxide aerogel (3D rGA) was synthesized by hydrothermal method and cobalt imidazolate framework-67 (ZIF-67) was further grown in situ on the 3D rGA matrix directly. The resultant ZIF-67@3D rGA nanocomposite was checked by different techniques including scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectrophotometry (FT-IR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and thermo-gravimetric analysis (TGA). The presence of 3D rGA acted as the backbone for the loading of ZIF-67, and the resultant ZIF-67@3D rGA nanocomposite exhibited an interconnected porous structure with large surface area and high conductivity due to synergistic effects, which was applied to the electrode modification and used for rutin detection.

[Genotype and phenotype studies on fetuses of 22q11.2 deletion syndrome].

Objective: To study the genotype and phenotype of fetuses with 22q11.2 microdeletion and other abnormalities such as cardiac malformation and cleft palate.

Methods: Fetal ultrasound was carried out at 12 weeks to 20 to 24 weeks of gestation.

Perchlorate bioreduction by anaerobic granular sludge immobilised with Fe-HA complex: Performance, extracellular polymeric substances and microbial community structure.

An iron-humic acid (Fe-HA) complex was used as a redox mediator in perchlorate (ClO) bioreduction. Bioreduction performance, extracellular polymeric substances (EPS), and microbial community structure were comprehensively explored using different types of anaerobic granular sludge (AnGS) immobilised without the Fe-HA complex (AnGS) and with the Fe-HA complex (AnGS). The ClO was completely removed by AnGS by day 20, while the ClO was completely removed by AnGS by day 6.

ZnO-reduced graphene oxide composite based photoelectrochemical aptasensor for sensitive Cd(II) detection with methylene blue as sensitizer.

In this paper a photoelectrochemical (PEC) aptasensor based on specific recognition with conformational changed after the target Cd(II) identification was fabricated. A ZnO and reduced graphene oxide (ZnO-rGO) nanocomposite with enhanced PEC activity was designed as photoactive material. After the further incorporation of gold nanoparticles (AuNPs) with ZnO-rGO nanocomposite, the enhanced photocurrent signal could be detected owing to the localized surface plasmon resonance and good conductivity of AuNPs.

Synthesis and utilization of CoO doped carbon nanofiber for fabrication of hemoglobin-based electrochemical sensor.

In this paper cobalt oxide (CoO) nanoparticles were mixed with polyacrylonitrile to prepare CoO doped carbon nanofiber (CNF) composite by electrospinning and carbonization, which was further used to modify on carbon ionic liquid electrode (CILE). Hemoglobin (Hb) was immobilized on CoO-CNF/CILE surface with Nafion acted as the protective film to fabricate an electrochemical biosensor (Nafion/Hb/CoO-CNF/CILE). Electrochemical behavior of Hb on the electrode was investigated with a pair of quasi-reversible redox peak appeared on cyclic voltammogram and electrochemical parameters were calculated.

Photoelectrochemical aptasensor for lead(II) by exploiting the CdS nanoparticle-assisted photoactivity of TiO nanoparticles and by using the quercetin-copper(II) complex as the DNA intercalator.

A photoelectrochemical (PEC) aptasensor for Pb(II) detection is described. A nanocomposite consisting of CdS (2.5 μm) and TiO nanoparticles (10 nm) was used as a photoactive material, and gold nanochains (Au NCs) as the support for immobilization of the Pb(II)-binding aptamer.