Integration strategies involving 16S rDNA sequencing combined with untargeted metabolomics revealed the mechanism of Selaginella tamariscina (Beauv.) Spring in db/db diabetic mice.

Selaginella tamariscina extracts (STS), total flavonoids of Selaginella tamariscina (TFST) and the main active component amentoflavone (AMT) have hypoglycaemic-mitigating effects, but their efficacy and mechanism of action in db/db mice are unknown. This study aimed to evaluate the hypoglycaemic effects of Selaginella tamariscina and its extracts in db/db diabetic mice and explore their mechanisms through gut microbiota modulation and metabolomics. Sixty male db/db mice were divided into model (db/db), STS (1.

Diurnal variation characteristics and meteorological causes of autumn ozone in the Pearl River Delta, China.

This study investigates the diurnal variation of ozone (O) in the Pearl River Delta (PRD) during autumn from 2016 to 2021, focusing on the main O modes and their relationship with meteorological conditions. Utilizing K-means clustering, four patterns of O variation were identified: Cluster 1 (extremely low O), Cluster 2 (close to autumn average), Cluster 3 (abnormally high O at night), and Cluster 4 (extremely high O). In Cluster 1, the PRD was situated on the northwest side of the western Pacific subtropical high (WPSH), resulting in increased cloud cover, weakened radiation, and the lowest O growth rate during the day, with weak nighttime changes.

Clinicopathological value of the upregulation of cyclin-dependent kinases regulatory subunit 2 in osteosarcoma.

Background: Cyclin-dependent kinase subunit 2 (CKS2) is a member of cyclin dependent kinase subfamily and the relationship between CKS2 and osteosarcoma (OS) remains to be further analyzed.

Methods: 80 OS and 41 non-tumor tissue samples were arranged to perform immunohistochemistry (IHC) to evaluate CKS2 expression between OS and non-tumor samples. The standard mean deviation (SMD) was calculated based on in-house IHC and tissue microarrays, and exterior high-throughput datasets for further verification of CKS2 expression trend in OS.

The Clinical Significance and Potential Molecular Mechanism of Upregulated CDC28 Protein Kinase Regulatory Subunit 1B in Osteosarcoma.

Background: CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B) is a member of cyclin-dependent kinase subfamily and the relationship between CKS1B and osteosarcoma (OS) remains to be explored.

Methods: 80 OS and 41 nontumor tissue samples were arranged to conduct immunohistochemistry (IHC) to evaluate CKS1B expression between OS and nontumor samples. The standard mean deviation (SMD) was calculated based on in-house IHC and tissue microarrays and exterior high-throughput datasets for further verification of CKS1B expression in OS.