Venous Endothelial Cell Transcriptomic Profiling Implicates METAP1 in Preeclampsia.

Background: Preeclampsia is a hypertensive disorder of pregnancy characterized by systemic endothelial dysfunction. The pathophysiology of preeclampsia remains incompletely understood. This study used human venous endothelial cell (EC) transcriptional profiling to investigate potential novel mechanisms underlying EC dysfunction in preeclampsia.

Bradycardia-dependent conduction block of the atrial tissue in a patient after double-chamber implantable cardioverter defibrillator implantation.

Seldom are reports of phase 4 block or bradycardia-dependent conduction block in atrial tissue found in the literature. Here, we describe the case of a patient with sick sinus syndrome with Torsade de Pointes who, following the implantation of a double-chamber implantable cardioverter defibrillator, developed intra-atrial bradycardia-dependent conduction block. The patient's optimal pacing parameters were achieved by raising the rate.

Disrupting methyl-CpG-binding protein 2 expression induces the transformation of induced pluripotent stem cell cardiomyocytes into pacemaker-like cells by insulin gene enhancer binding protein 1.

Background: The experiment will explore whether interfering with the expression of methyl-CpG-binding protein 2 (MecP2) can enhance the ability of insulin gene enhancer binding protein 1 (ISL1) to induce iPSC-CMs to differentiate into pacemaker-like cells.

Methods: Differentiation of induced pluripotent stem cells (iPSCs) into cardiomyocytes (CMs) can be induced via the regulation of the Wnt signaling pathway. Real-time quantitative PCR (qPCR), western blotting, immunofluorescence staining, and patch-clamp technique were used to analyze the ability of ISL1 to induce the transformation of iPSC-CMs into pacemaker-like cells.

A Comparative Study of Severe and Critical Influenza B in Children in the 2021-2022 Winter Season.

Introduction: Influenza B viruses are less common than influenza A viruses in most seasons and cause relatively milder forms of infection that are less studied. We witnessed a dominance of influenza B in Shijiazhuang, China, in the 2021-2022 winter season. In this study, we comparatively investigated the severe and critical influenza B in pediatric patients.

Association between chronic periodontitis and the risk of Alzheimer's disease: combination of text mining and GEO dataset.

Background: Although chronic periodontitis has previously been reported to be linked with Alzheimer's disease (AD), the pathogenesis between the two is unclear. The purpose of this study is to analyze and screen the relevant and promising molecular markers between chronic periodontitis and Alzheimer's disease (AD).

Methods: In this paper, we analyzed three AD expression datasets and extracted differentially expressed genes (DEGs), then intersected them with chronic periodontitis genes obtained from text mining, and finally obtained integrated DEGs.

Integrative analysis of key candidate genes and signaling pathways in acute coronary syndrome related to obstructive sleep apnea by bioinformatics.

Although obstructive sleep apnea (OSA) has been clinically reported to be associated with acute coronary syndrome (ACS), the pathogenesis between the two is unclear. Herein, we analyzed and screened out the prospective molecular marker. To explore the candidate genes, as well as signaling cascades involved in ACS related to OSA, we extracted the integrated differentially expressed genes (DEGs) from the intersection of genes from the Gene Expression Omnibus (GEO) cohorts and text mining, followed by enrichment of the matching cell signal cascade through DAVID analysis.

Development of an Immune-Related Prognostic Index Associated With Glioblastoma.

Although the tumor microenvironment (TME) is known to influence the prognosis of glioblastoma (GBM), the underlying mechanisms are not clear. This study aims to identify hub genes in the TME that affect the prognosis of GBM. The transcriptome profiles of the central nervous systems of GBM patients were downloaded from The Cancer Genome Atlas (TCGA).

Computational screening of potential glioma-related genes and drugs based on analysis of GEO dataset and text mining.

Background: Considering the high invasiveness and mortality of glioma as well as the unclear key genes and signaling pathways involved in the development of gliomas, there is a strong need to find potential gene biomarkers and available drugs.

Methods: Eight glioma samples and twelve control samples were analyzed on the GSE31095 datasets, and differentially expressed genes (DEGs) were obtained via the R software. The related glioma genes were further acquired from the text mining.

QTL mapping of selenium content using a RIL population in wheat.

Selenium (Se) is an essential trace element that plays various roles in human health. Understanding the genetic control of Se content and quantitative trait loci (QTL) mapping provide a basis for Se biofortification of wheat to enhance grain Se content. In the present study, a set of recombinant inbred lines (RILs) derived from two Chinese winter wheat varieties (Tainong18 and Linmai6) was used to detect QTLs for Se content in hydroponic and field trials.

[Effects of potassium on nitrogen translocation and distribution and nitrogen metabolism enzyme activities of sweet potato.].

A pot experiment with N tracing techniques was designed to study the effect of potassium application on nitrogen transfer, photosynthetic characteristics and nitrogen metabolism enzyme activities in two different growth stages of sweet potato. Results indicated that potassium application significantly increased the shoot N distribution rate. Compared with control, the N transfer rate of K treatment increased by 76.

Distribution of phthalate esters in agricultural soil with plastic film mulching in Shandong Peninsula, East China.

The content of phthalate esters (PAEs) was investigated in 36 vegetable fields with plastic film mulching in Shandong Peninsula, East China. Soils at depths of 0-10 cm, 10-20 cm, and 20-40 cm were collected, and 16 PAEs were analyzed by gas chromatography-mass spectrometry. PAEs were detected in all the analyzed samples.

Comparison of the interferon-gamma release assay with the traditional methods for detecting Mycobacterium tuberculosis infection in children.

The purpose of the article is to compare the whole blood interferon-γ release assay (IGRA) with the traditional methods for detecting Mycobacterium tuberculosis (MTB) infection in children. Fifteen childhood patients with tuberculosis and 15 healthy children were recruited. Sputa samples and venous blood were collected, and according to different procedures, IGRA, sputum smear, colloidal gold assay (CGA), fluorescence quantitation polymerase chain reaction (FQ-PCR), and tuberculosis skin test (TST) were, respectively, performed.

[Protective effects of total epimedium flavonoids against QA-induced toxicity in SH-SY5Y cells].

Objective: To investigate the protective effects and its mechanisms of total epimedium flavonoids (TEF) against Quindinince acid (QA)-induced toxicity in SH-SY5Y cells.

Methods: The MT assay was used to detect the toxicity of QA and the cell viability of pretreatment of TEF and co-incubation with QA in SH-SY5Y cells; Cell apoptosis was observed by Hochest33258 staining; SOD and GSH-Px activities as well as MDA content were measured by colorimetric method; The mitochondrial membrane potential (delta psi m) and intracellular free calcium concentration ([Ca2+]i) were monitored by FCM.

Results: TEF increased the cell viability of SH-SY5Y cells, reduced apoptosis cells significantly, improved SOD and GSH-Px activities, decreased MDA content, increased the mitochondrial membrane potential and reduced intracellular free calcium concentration( [Ca2+]i ).

Phthalic acid esters in soils from vegetable greenhouses in Shandong Peninsula, East China.

Soils at depths of 0 cm to 10 cm, 10 cm to 20 cm, and 20 cm to 40 cm from 37 vegetable greenhouses in Shandong Peninsula, East China, were collected, and 16 phthalic acid esters (PAEs) were detected using gas chromatography-mass spectrometry (GC-MS). All 16 PAEs could be detected in soils from vegetable greenhouses. The total of 16 PAEs (Σ16PAEs) ranged from 1.

[Distribution characteristics of phthalic acid esters in soils and peanut kernels in main peanut producing areas of Shandong Province, China].

Surface soil (0-20 cm) and peanut kernel samples were collected in four main peanut producing areas of Shandong Province, and the contents of six PAEs chemicals that classified by the U. S. Environmental Protection Agency as priority pollutants were determined by gas chromatography (GC).

[Phthalic acid esters (PAEs) pollution in farmland soils: a review].

The environmental pollution and food safety problems caused by phthalic acid esters (PAEs) have been attracted 'extensive attention around the world. As a large PAEs producer and consumer, China is facing severe PAEs environmental pollution problems. This paper reviewed the present pollution status of six PAEs classified by the U.

Comparing TCR beta chain variable gene profile skewness between children with tuberculosis and BCG-vaccinated children.

Background: We compared the T cell antigen receptor (TCR-BV) gene families of peripheral blood mononuclear cells (PMBC) between children with tuberculosis (TB) and those inoculated with the Bacille Calmette Guerin (BCG) vaccine.

Methods: The total RNA was extracted from PMBC of 15 TB children, 15 BCG-vaccinated children and 15 healthy controls. The RNAs were reverse-transcribed and amplified by polymerase chain reaction (PCR).

[Matrix solid-phase dispersion and gas chromatography/mass spectrometry for the determination of phthalic acid esters in vegetables].

A method based on matrix solid-phase dispersion (MSPD) and gas chromatography/mass spectrometry (GC/MS) to determine dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl phthalate (DBP), butylbenzyl phthalate (BBP) and di-2-ethylhexyl phthalate (DEHP) in vegetables was developed. The method, which required Florisil as solid dispersion sorbent, graphitized carbon black as purificant and ethyl acetate as eluting solvent, is simple, fast and suitable for the preparation of a large number of samples. The results showed that linearity of response over the five phthalic acid esters was good from 0.