Elucidating the downstream pathways triggered by HS signaling in under drought stress via transcriptome analysis.

Hydrogen sulfide (HS) is a crucial signaling molecule in plants. Recent studies have shown that HS plays an equally important role as nitric oxide (NO) and hydrogen peroxide (HO) in plant signaling. Previous studies have demonstrated the involvement of HS in regulating drought and other stressful environmental conditions, but the exact downstream molecular mechanisms activated by the HS signaling molecule remain unclear.

Exogenous hydrogen sulphide promotes plant flowering through the Arabidopsis splicing factor AtU2AF65a.

Alternative splicing (AS) is an important regulatory mode at the post-transcriptional level, through which many flowering genes regulate floral transition by producing multiple transcripts, and splicing factors have essential roles in this process. Hydrogen sulphide (HS) is a newly found gasotransmitter that has critical physiological roles in plants, and one of its potential modes of action is via persulfidation of target proteins at specific cysteine sites. Previously, it has been shown that both the splicing factor AtU2AF65a and HS are involved in the regulation of plant flowering.

Transcriptomic analysis reveals the functions of HS as a gasotransmitter independently of Cys in .

Numerous studies have revealed the gasotransmitter functions of hydrogen sulfide (HS) in various biological processes. However, the involvement of HS in sulfur metabolism and/or Cys synthesis makes its role as a signaling molecule ambiguous. The generation of endogenous HS in plants is closely related to the metabolism of Cys, which play roles in a variety of signaling pathway occurring in various cellular processes.

HS-mediated balance regulation of stomatal and non-stomatal factors responding to drought stress in Chinese cabbage.

Increased evidence has shown that hydrogen sulfide (HS), a novel gasotransmitter, could enhance drought resistance in plants by inducing stomatal closure, with concurrent enhancement of photosynthetic efficiency, but little is known about the mechanism behind this contradictory phenomenon. This study examined the regulating mechanism of HS in response to drought stress from stomatal and non-stomatal factors in Chinese cabbage. The results showed that exogenous HS could increase the accumulation of photosynthetic pigments and alleviate the damage caused by drought stress.

Functional characterization of the Serine acetyltransferase family genes uncovers the diversification and conservation of cysteine biosynthesis in tomato.

Sulfur-containing compounds are essential for plant development and environmental adaptation, and closely related to the flavor and nutrition of the agricultural products. Cysteine, the first organic sulfur-containing molecule generated in plants, is the precursor for most of these active substances. Serine acetyltransferase (SERAT) catalyzes the rate-limiting step of its formation.

Gasotransmitter HS accelerates seed germination via activating AOX mediated cyanide-resistant respiration pathway.

Hydrogen sulfide (HS) has been witnessed as a crucial gasotransmitter involving in various physiological processes in plants. HS signaling has been reported to involve in regulating seed germination, but the underlying mechanism remains poorly understood. Here, we found that endogenous HS production was activated in germinating Arabidopsis seeds, correlating with upregulated both the transcription and the activity of L-cysteine desulfhydrase (EC 4.

An atlas of plant full-length RNA reveals tissue-specific and monocots-dicots conserved regulation of poly(A) tail length.

Poly(A) tail is a hallmark of eukaryotic messenger RNA and its length plays an essential role in regulating mRNA metabolism. However, a comprehensive resource for plant poly(A) tail length has yet to be established. Here, we applied a poly(A)-enrichment-free, nanopore-based method to profile full-length RNA with poly(A) tail information in plants.

AtPRMT5-mediated AtLCD methylation improves Cd2+ tolerance via increased H2S production in Arabidopsis.

Arabidopsis (Arabidopsis thaliana) PROTEIN ARGININE METHYLTRANSFERASE5 (PRMT5), a highly conserved arginine (Arg) methyltransferase protein, regulates multiple aspects of the growth, development, and environmental stress responses by methylating Arg in histones and some mRNA splicing-related proteins in plants. Hydrogen sulfide (H2S) is a recently characterized gasotransmitter that also regulates various important physiological processes. l-cysteine desulfhydrase (LCD) is a key enzyme of endogenous H2S production.

Identification and functional characterization of a cystathionine β-lyase (CBL) enzyme for HS production in Arabidopsis thaliana.

Sulfide or sulfur metabolism plays an important role in the growth and development of plants. Cystathionine β-lyase (CBL) is an important enzyme in methionine synthesis, but a comprehensive understanding of CBL functions is limited. As the third gasotransmitter, hydrogen sulfide (HS) plays important physiological roles in plants.

Hydrogen sulfide inhibits the abscission of tomato pedicel through reconstruction of a basipetal auxin gradient.

Abscission is an important developmental process and an essential agricultural trait. Auxin and ethylene are two phytohormones with important roles in the complex, but still elusive signaling network of abscission. Here, we found that hydrogen sulfide (HS), a newly identified gasotransmitter, inhibits the initiation of tomato pedicel abscission.

Profiling Cystathionine β/γ-Lyase in Complex Biosamples Using Novel Activatable Fluorogens.

Cystathionine lyase, the key enzyme in transsulfuration and reverse transsulfuration pathways, is involved in a wide array of physiological and pathophysiological processes in both mammals and nonmammals. Though the biological significance of the hydrogen sulfide/cystathionine lyase system in disease states is extensively discussed, the absence of molecular methods for direct monitoring of cystathionine lyase in complex biosamples renders the result unreliable and perplexing. Here, we present the first attempt at designing and developing effective activatable fluorescent probes for cystathionine lyase based on the naphthylamide scaffold.

Lipidomic, Transcriptomic, and BSA-660K Single Nucleotide Polymorphisms Profiling Reveal Characteristics of the Cuticular Wax in Wheat.

Plant epidermal wax helps protect plants from adverse environmental conditions, maintains the function of tissues and organs, and ensures normal plant development. However, the constituents of epidermal wax and the regulatory mechanism of their biosynthesis in wheat have not been fully understood. Wheat varieties with different wax content, Jinmai47 and Jinmai84, were selected to comparatively analyze their waxy components and genetic characteristics, using a combination of lipidomic, transcriptomic, and BSA-Wheat 660K chip analysis.

HS aids osmotic stress resistance by S-sulfhydration of melatonin production-related enzymes in Arabidopsis thaliana.

Hydrogen sulfide closed Arabidopsis thaliana stomata by increasing the transcription of melatonin-producing enzymes and the post-translational modification levels to combat osmotic stress. Hydrogen sulfide (HS) and melatonin (MEL) reportedly have similar functions in many aspects of plant growth, development and stress response. They regulate stomatal movement and enhance drought resistance.

The GA 20-Oxidase Encoding Gene Controls the Main Stem Elongation in .

Plant height is an important agronomic trait that is closely related to biomass yield and crop production. Despite legumes comprise one of the largest monophyletic families that are second only to grasses in terms of economic and nutritional values, due to an ancient genome duplication event, most legume plants have complex genomes, thus the molecular mechanisms that determine plant height are less known in legumes. Here, we report the identification and characterization of (), which is required for the plant height in the model legume .

Cystathionine gamma-lyase/H S signaling facilitates myogenesis under aging and injury condition.

Hydrogen sulfide (H S) can be endogenously produced and belongs to the class of signaling molecules known as gasotransmitters. Cystathionine gamma-lyase (CSE)-derived H S is implicated in the regulation of cell differentiation and the aging process, but the involvements of the CSE/H S system in myogenesis upon aging and injury have not been explored. In this study, we demonstrated that CSE acts as a major H S-generating enzyme in skeletal muscles and is significantly down-regulated in aged skeletal muscles in mice.

HS Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis.

Hydrogen sulfide (HS) is a gasotransmitter along with nitric oxide and carbon oxide, which is involved in plant growth and development as well as biotic and abiotic stress resistance. In a previous study, we reported that mitogen-activated protein kinases, especially MPK4, are important downstream components of HS involved in alleviating cold stress; however the underlying mechanism is unclear. In this study, we determined that the ability of HS to alleviate cold stress is impaired in mutants, but not in the upstream and mutants.

Hydrogen sulfide promotes flowering in heading Chinese cabbage by S-sulfhydration of BraFLCs.

Heading Chinese cabbage (Brassica rapa L. syn. B.

ATP-Binding Cassette G Transporters SGE1 and MtABCG13 Control Stigma Exsertion.

Stigma exsertion is an important agricultural trait that facilitates the application of heterosis in crop breeding. Although several quantitative trait loci associated with stigma exsertion have been fine-mapped or cloned, the underlying genetic basis, particularly in legumes, remains unclear. In this study, we identified and characterized the exserted stigma mutant () in the model legume The exserted stigma phenotype of is mainly caused by physical interaction between floral organs, in which normal petal and stamen elongation are inhibited due to flower cuticle defects.

[Prokaryotic expression, purification and functional identification of epidermal pattern factors in Arabidopsis thaliana].

Stomatal density is important for crop yield. In this paper, we studied the epidermal pattern factors (EPFs) related to stomatal development. Prokaryotic expression vectors were constructed to obtain EPFs.

Cystathionine gamma-lyase/HS system suppresses hepatic acetyl-CoA accumulation and nonalcoholic fatty liver disease in mice.

Aims: Hydrogen sulfide (HS) as a novel gasotransmitter can be endogenously produced in liver by cystathionine gamma-lyase (CSE). The dysfunctions of CSE/HS system have been linked to various liver diseases. Acetyl-CoA is the key intermediate from the metabolism of lipid.

Hydrogen sulfide inhibits ethylene-induced petiole abscission in tomato ( L.).

Abscission is a dynamic physiological process that is ubiquitous in plants and can also be an essential agronomic trait in crops, thus attracting attention from plant growers and breeders. In general, the process of plant organ abscission can be divided into four steps, among which the step to obtain the competence to respond to abscission signals (step 2) is the most complex; however, the molecular mechanism underlying this process remains unclear. In this study, we found that hydrogen sulfide (HS) inhibited the abscission of the tomato petiole in a dose-dependent manner, and the abscission of the petiole was accelerated when an HS scavenger was applied.

SMALL LEAF AND BUSHY1 controls organ size and lateral branching by modulating the stability of BIG SEEDS1 in Medicago truncatula.

Organ size is a major agronomic trait that determines grain yield and biomass production in crops. However, the molecular mechanisms controlling organ size, especially in legumes, are poorly understood. Using forward genetic approaches in a Tnt1 insertion mutant population of the model legume Medicago truncatula, we identified SMALL LEAF AND BUSHY1 (SLB1), which is required for the control of organ size and lateral branching.

Golgi Stress Response, Hydrogen Sulfide Metabolism, and Intracellular Calcium Homeostasis.

The physiological and pathological importance of hydrogen sulfide (HS) as a novel gasotransmitter has been widely recognized. Cystathionine gamma-lyase (CSE) is one of the major HS-producing enzymes and it regulates diverse functions in connection with intracellular calcium (Ca). The aim of this study is to examine the role of HS in Golgi stress-related cell injury and skeletal muscle disorders.

Vinegar residue supported nanoscale zero-valent iron: Remediation of hexavalent chromium in soil.

A composite material comprising of nanoscale zero-valent iron (nZVI) supported on vinegar residue (nZVI@VR) was prepared and applied for remediation of soils contaminated by hexavalent chromium (Cr(VI)). Sedimentation test results revealed that the nZVI@VR displayed enhanced stability in comparison to the bare-nZVI. Remediation experiments exhibited the immobilization efficiency of Cr(VI) and Cr was 98.