Constructing fecal-derived electrocatalysts for CO upcycling: simultaneously tackling waste and carbon emissions.

The escalating global fecal waste and rising CO levels present dual significant environmental challenges, further intensified by urbanization. Traditional fecal waste management methods are insufficient, particularly in addressing the related health risks and environmental threats. This study explores the synthesis of biochar from pig manure as a carbon substrate to disperse and stabilize Cu nanoparticles, resulting in the formation of an efficient Cu-NB-2000 electrocatalyst for electrocatalytic CO reduction (ECR).

Studies on the metabolism of paeoniflorin in human intestinal microflora by high performance liquid chromatography/electrospray ionization/Fourier transform ion cyclotron resonance mass spectrometry and quadrupole time-of-flight mass spectrometry.

In this study, a high performance liquid chromatography/electrospray ionization/Fourier transform ion cyclotron resonance mass spectrometry and quadrupole time-of-flight mass spectrometry (HPLC-ESI-FT-ICR MS and HPLC-ESI-QTOF MS) based on chemical profiling method was established to study the metabolites of paeoniflorin in human intestinal microflora (HIM). By virtue of the high resolution, high speed of HPLC and the accurate mass measurement of FT-ICR MS and QTOF MS, 31 metabolites in methyl tert-butyl ether (MTBE) layer were detected, and the structures of 16 metabolites were identified. Among them, 13 metabolites (including two new compounds) were found for the first time in HIM transformation in vitro.

[Determination of sedative and β-receptor hormone residues in feed by ultra high performance liquid chromatography-tandem mass spectrometry].

An analysis and detection method for eight sedatives and fifteen β-receptor hormones in feed was established by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The sample was extracted with acetonitrile-1% (v/v) trichloroacetic acid aqueous solution (7:3, v/v), and purified by cationic solid phase extraction column. The extracts were separated on an Agilent Zorbax Eclipse Plus C chromatographic column (100 mm×3.

Study on the interaction of paeoniflorin with human serum albumin (HSA) by spectroscopic and molecular docking techniques.

The interaction of paeoniflorin with human serum albumin (HSA) was investigated using fluorescence, UV-vis absorption, circular dichroism (CD) spectra and molecular docking techniques under simulative physiological conditions. The results clarified that the fluorescence quenching of HSA by paeoniflorin was a static quenching process and energy transfer as a result of a newly formed complex (1:1). Paeoniflorin spontaneously bound to HSA in site I (subdomain IIA), which was primarily driven by hydrophobic forces and hydrogen bonds (ΔH° = - 9.

Metabolic study of paeoniflorin and total paeony glucosides from Paeoniae Radix Rubra in rats by high-performance liquid chromatography coupled with sequential mass spectrometry.

A clear understanding of the metabolism of Traditional Chinese Medicines is extremely important in their rational clinical application and effective material foundation research. A novel and reliable strategy was performed to find more metabolites of paeoniflorin, determine the metabolites of total paeony glucosides (TPG) by means of determining those metabolites of paeoniflorin, and compare the metabolism differences between paeoniflorin and TPG by intragastric administration. This strategy was characterized as follows.

Probing the binding reaction of cytarabine to human serum albumin using multispectroscopic techniques with the aid of molecular docking.

Cytarabine is a kind of chemotherapy medication. In the present study, the molecular interaction between cytarabine and human serum albumin (HSA) was investigated via fluorescence, UV-vis absorption, circular dichroism (CD) spectroscopy and molecular docking method under simulative physiological conditions. It was found that cytarabine could effectively quench the intrinsic fluorescence of HSA through a static quenching process.

Effects of different metal ions on the fluorescence of CdSe/ZnS quantum dots capped with various thiolate ligands.

In this report, we systematically studied the effects of different metal ions on the fluorescence of common thiolate ligand capped quantum dots. Generally, heavy metal ions exhibited much more significant fluorescence quenching. Also, the coordination capability and steric hindrance of the ligand jointly affect the quenching efficiency.