The de novo missense mutation F224S in GABRB2, identified in epileptic encephalopathy and developmental delay, impairs GABAR function.

Genetic variants in genes encoding subunits of the γ-aminobutyric acid-A receptor (GABAR) have been found to cause neurodevelopmental disorders and epileptic encephalopathy. In a patient with epilepsy and developmental delay, a de novo heterozygous missense mutation c.671 T > C (p.

NODULATION TRIO in Medicago truncatula: Unveiling the redundant roles of MtLYK2, MtLYK3, and MtLYK2bis.

Three Medicago truncatula LysM domain receptor kinases have redundant functions in nodulation, with multiple specificities mediating both entry and signaling responses and with distinct contributions to nodulation likely resulting from differing transcription patterns.

Changes in microbial composition explain the contrasting responses of glucose and lignin decomposition to soil acidification in an alpine grassland.

Soil acidification often suppresses microbial growth and activities, resulting in a negative impact on soil organic carbon (C) decomposition. While the detrimental effects of acidification on soil and plant properties have been extensively studied, less attention has been paid on the shifts in soil microbial communities and their influences of the decomposition of organic C with different chemical complexities. Taking advantage of an acid addition experiment in a Tibetan alpine meadow, here we examined the response of soil microbial communities to soil acidification and microbial effect on the decomposition of organic C with different chemical complexities (i.

Periplaneta americana extract attenuates hepatic fibrosis progression by inhibiting collagen synthesis and regulating the TGF-β1/Smad signaling pathway.

Introduction: Liver fibrosis is the damage repair response following chronic liver diseases. Activated hepatic stellate cells (HSCs) are the main extracellular matrix (ECM)-producing cells and key regulators in liver fibrosis. Periplaneta americana shows prominent antifibrotic effects in liver fibrosis; however, the underlying mechanisms remain undetermined.

Molecular Cloning, Expression Analysis, and Functional Analysis of Nine in (L.) Lam.

Sugar Will Eventually be Exported Transporter () genes play an important regulatory role in plants' growth and development, stress response, and sugar metabolism, but there are few reports on the role of SWEET proteins in sweet potato. In this study, nine genes were obtained via PCR amplification from the cDNA of sweet potato. Phylogenetic analysis showed that nine IbSWEETs separately belong to four clades (Clade I~IV) and contain two MtN3/saliva domains or PQ-loop superfamily and six~seven transmembrane domains.

Silencing of CPNE1-TRAF2 Axis Restrains the Development of Pancreatic Cancer.

Background: Copine 1 (CPNE1) acts as a promoter in the progression of many kinds of cancers with the exception of pancreatic cancer (PC). This research is designed to probe the function of the CPNE1-tumor necrosis factor receptor-associated factor 2 (TRAF2) axis in PC.

Methods: and models of PC were constructed, and a series of biological function tests, including MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], colony formation, flow cytometry, and immunohistochemistry, were performed.

Dual RNA-Seq Analysis Pinpoints a Balanced Regulation between Symbiosis and Immunity in - Symbiotic Nodules.

Legume-rhizobial symbiosis initiates the formation of root nodules, within which rhizobia reside and differentiate into bacteroids to convert nitrogen into ammonium, facilitating plant growth. This process raises a fundamental question: how is plant immunity modulated within nodules when exposed to a substantial number of foreign bacteria? In , a mutation in the () gene exclusively results in the formation of necrotic nodules combined with activated immunity, underscoring the critical role of in suppressing immunity within nodules. In this study, we employed a dual RNA-seq transcriptomic technology to comprehensively analyze gene expression from both hosts and symbionts in the mutant nodules at different developmental stages (6 dpi and 10 dpi).

Molecular and Clinicopathological Characteristics of Lung Cancer Concomitant Chronic Obstructive Pulmonary Disease (COPD).

Introduction: Chronic obstructive pulmonary disease (COPD) and lung cancer often coexist, but its pathophysiology and genomics features are still unclear.

Methods: In this study, we retrospectively collected lung cancer concomitant COPD (COPD-LC) and non-COPD lung cancer (non-COPD-LC) patients, who performed next generation sequencing (NGS) and had clinicopathological information simultaneously. The COPD-LC data from the TCGA cohort were collected to conduct further analysis.

Identification of anti-hepatic fibrosis components in Periplaneta americana based on spectrum-effect relationship and chemical component separation.

Periplaneta americana (PA) is used as a traditional medicine for hepatic diseases such as hepatic fibrosis in China. However, the relationship between the corresponding therapeutic effect and the chemical composition is still unclear. In this study, spectrum-effect relationship and chemical component separation were used to discover the potential of anti-hepatic fibrosis components of PA.

Application of insect-proof nets in pesticide-free rice creates an altered microclimate and differential agronomic performance.

Background: Insect-proof nets are commonly used in crop production and scientific research because of their environmental, economic, and agronomic benefits. However, insect-proof nets can unintentionally alter the microclimate inside the screenhouse and therefore greatly affect plant growth and yield. To examine the microclimate and agronomic performance of pesticide-free rice under insect-proof nets, two-year field experiments were carried out in 2011 and 2012.

Influences of climate change on area variation of Qinghai Lake on Qinghai-Tibetan Plateau since 1980s.

Qinghai-Tibetan Plateau is the most sensitive region to global warming on Earth. Qinghai Lake, the largest lake on the plateau, has experienced evident area variation during the past several decades. To quantify the area changes of Qinghai Lake, a satellite-based survey based on Landsat images from the 1980s to 2010s has been performed.

Biochar increases plant growth and alters microbial communities via regulating the moisture and temperature of green roof substrates.

Green roofs have increasingly been designed and applied to relieve environmental problems, such as water loss, air pollution as well as heat island effect. Substrate and vegetation are important components of green roofs providing ecosystem services and benefiting the urban development. Biochar made from sewage sludge could be potentially used as the substrate amendment for green roofs, however, the effects of biochar on substrate quality and plant performance in green roofs are still unclear.

[Physiological and ecological effects of sulfur fertilization on soybean].

A pot experiment with 3 levels of elemental sulfur (0, 30, and 60 mg S x kg(-1)) showed that sulfur fertilization on soybean increased the side roots number by 8.6% - 33.2%, root dry weight by 6.

[Effects of elemental sulphur and dicyandiamide on mitigating NO3- -N leaching loss from vegetable soil].

In a pot experiment with allium as test plant and NH4HCO3 as nitrogen source, this paper studied the effects of element sulphur (S0) and dicyandiamide (DCD) on mitigating the NO3- -N leaching loss from soil and on soil inorganic nitrogen (NO3- -N and NH4+ -N) content. The results showed that within the 12 weeks of the experiment, the cumulative leaching loss of soil NO3- -N in treatments S0 + DCD and S0 was 83%-86% and 83% lower, while that of soil NH4+ -N was 16.8-21.

[Dynamics of dry deposition velocities (Vd) of atmospheric SO2 on rapeseed/rice rotation systems in selected area of south China].

With the profile meteorological data collected from the farmland micro-meteorological experimental substation, Experiment Station of Red Earth Ecology, Yingtan, Jiangxi, Chinese Academy of Sciences during rapeseed/rice rotation from Nov. 1998 to Oct. 1999, the surface layer turbulence characteristic parameters (u*, theta*, L) and the atmospheric SO2 dry deposition velocities (Vd) were calculated.