Microbial community composition and cooccurrence patterns driven by co-contamination of arsenic and antimony in antimony-mining area.

In the current study, a typical Sb mine was selected to explore the microbial community composition and assembly driven by the cocontamination of As/Sb with geographic distance. Our results showed that environmental parameters, especially pH, TOC, nitrate, total and bioavailable As/Sb contents largely affected the microbial community diversity and composition. The total and bioavailable As/Sb levels were significantly positively correlated with the relative abundance of Zavarzinella, Thermosporothrix and Holophaga, while the pH presented a significant negative correlation with the three genera, potentially implying they are important taxonomic groups in acid-mining soils.

Safety evaluation of RNAi transgenic L. based on microbial diversity and metabonomic analysis.

Oleic acid desaturase () is the key enzyme that produces polyunsaturated fatty acids in rapeseed ( L), which is one of the main oil crops. RNA interference (RNAi) is an emerging technique that provides new opportunities for the generation of new traits in plants. To increase oleic acid content and reduce linoleic and linolenic acid content in rapeseed, we constructed an ihpRNA plant expression vector of the gene and obtained transgenic plants for multiple generations by stable inheritance.

A photoelectrochemical self-powered sensor for the detection of sarcosine based on NiO NSs/PbS/Au NPs as photocathodic material.

In this study, lead(II) sulfide (PbS) nanocrystals were modified on nickel(II)oxide nanosheets (NiO NSs) via the chemical bath method. Afterwards, Au nanoparticles (NPs) were also modified successfully. A photoelectrochemical (PEC) self-powered platform for detecting sarcosine with high PEC activity was constructed.

The migration, transformation, and risk assessment of heavy metals in residue and bio-oil obtained by the liquefaction of pig manure.

The total contents and chemical speciation analysis of Zn, Cu, Pb, Cr, Mn, Ni, Cd, and As in pig manure (PM), liquefaction residues (LRs), and bio-oils (BOs) derived from PM by liquefaction with ethanol as a solvent at 180-300 °C were thoroughly investigated in this study. The environment risk assessment, leachability, and bioavailability of heavy metals in PM and LRs were studied. The results showed that more than 75% of heavy metals remained in LRs.

Effects of different stoichiometric ratios on mineralisation of root exudates and its priming effect in paddy soil.

In paddy soil, the root exudates strongly influence the microbial activity and soil organic matter (SOM) mineralisation. However, the stoichiometric regulation of the mineralisation of root exudates and their priming effect on paddy soil remains unclear. Thus, we used manipulative laboratory incubations to measure the mineralisation of root exudates and the subsequent priming effect in paddy soil under different stoichiometric conditions.

Preparation and characterization of 0D Au NPs@3D BiOI nanoflower/2D NiO nanosheet array heterostructures and their application as a self-powered photoelectrochemical biosensing platform.

In this work, we demonstrate that zero-dimensional Au nanoparticles (0D Au NPs)-decorated three-dimensional bismuth oxyiodide (BiOI) nanoflower (3D BiOI NFs)/two-dimensional nickel oxide (NiO) nanosheet array (2D NiO NSAs) hybrid nanostructures can be used as a self-powered cathodic photoelectrochemical (PEC) biosensing platform. The formation of 3D BiOI NFs on 2D NiO NSAs was carried out by a chemical bath deposition method, while 0D Au NPs were coated on 3D BiOI NFs/2D NiO NSAs through a dip-coating method. Subsequently, glucose oxidase (GOD) as an enzyme model was immobilized on the surface of a Au@BiOI/NiO electrode the adhesion of poly-(diallyldimethylammonium chloride) (PDDA).

Repression of miR-143 mediates Cr (VI)-induced tumor angiogenesis via IGF-IR/IRS1/ERK/IL-8 pathway.

Hexavalent chromium [Cr (VI)] is a well-known human carcinogen associated with the increased risk of lung cancer. However, the mechanism underlying the Cr (VI)-induced carcinogenesis remains unclear due to the lack of suitable experimental models. In this study, we developed an in vitro model by transforming nontumorigenic human lung epithelial BEAS-2B cells through long-term exposure to Cr (VI).

A regulatory circuit of miR-148a/152 and DNMT1 in modulating cell transformation and tumor angiogenesis through IGF-IR and IRS1.

Dysregulation of microRNAs is a common feature in human cancers, including breast cancer (BC). Here we describe the epigenetic regulation of miR-148a and miR-152 and their impact on BC cells. Due to the hypermethylation of CpG island, the expression levels of both miR-148a and miR-152 (miR-148a/152) are decreased in BC tissues and cells.

MiR-145 inhibits tumor angiogenesis and growth by N-RAS and VEGF.

MiR-145 is known as a tumor suppressor in numerous human cancers. However, its role in tumor angiogenesis remains poorly defined. In this study, we found that miR-145 was significantly downregulated in breast cancer tissues by using 106 cases of normal and cancer tissues as well as in breast cancer cells.

Peritoneal relative to venous serum biomarker concentrations for diagnosis of ectopic pregnancy.

Purpose: To retrospectively analyze the relationships of peritoneal serum relative to venous serum (R (p/v)) ratios for human chorionic gonadotropin, CA-125, and creatine kinase to the ectopic pregnancy (EP).

Methods: Intra-abdominal fluid and venous blood samples of 118 subjects with suspected EP were obtained for biomarker measurements. R (p/v-hCG) >1 was considered indicative of EP, and final diagnosis was based on surgical finding of an ectopic chorionic villous or gynecological ultrasound finding of an intrauterine gestational sac.

Analysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer.

Purpose: To investigate expression, regulation, potential role and targets of miR-195 and miR-497 in breast cancer.

Experimental Design: The expression patterns of miR-195 and miR-497 were initially examined in breast cancer tissues and cell lines by Northern blotting and quantitative real-time PCR. Combined bisulfite restriction analysis and bisulfite sequencing were carried out to study the DNA methylation status of miR-195 and miR-497 genes.

microRNA-146b inhibits glioma cell migration and invasion by targeting MMPs.

MicroRNAs (miRNAs) are a class of endogenous, small non-protein coding single-stranded RNA molecules, which are crucial post-transcriptional regulators of gene expression. Previous studies have shown that miRNAs participate in a wide range of biological functions and play important roles in various human diseases including glioma. However, the role of miRNAs in mediating glioblastoma cell migration and invasion has not been elucidated.

MicroRNA-15b regulates cell cycle progression by targeting cyclins in glioma cells.

MicroRNAs (miRNAs) are non-protein-coding RNAs that function as post-transcriptional gene regulators. Recent evidence has shown that miRNA plays a pivotal role in the development of many cancers including glioma, a lethal brain cancer. We have recently compared the miRNA expression profiles between normal brain and glioma tissues from Chinese patients by miRNA microarray and identified a panel of differentially expressed miRNAs.

Two-dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinoma.

Diethylnitrosamine (DEN) is a known carcinogen that can alkylate DNA molecules. In rats, DEN-induced hepatocellular carcinoma (HCC) model is well established. In this study, we used a two-dimensional differential gel electrophoresis (2D-DIGE) system and liquid chromatography/mass spectrometry/mass spectrometry to identify the differential expression protein profiles between the DEN-induced HCC and healthy liver cells.

Preparation and biomedical application of a non-polymer coated superparamagnetic nanoparticle.

We report the preparation of a non-polymer coated superparamagnetic nanoparticle that is stable and biocompatible both in vitro and in vivo. The non-polymer, betaine, is a natural methylating agent in mammalian liver with active surface property. Upon systemic administration, the nanoparticle has preferential biodistribution in mammalian liver and exhibits good reduction of relaxivity time and negative enhancement for the detection of hepatoma nodules in rats using MRI.