Epigenetic modifications of histone H3 during the transdifferentiation of Thy-1(+) Lin(‑) bone marrow cells into hepatocytes.

The epigenetic modifications during the transdifferentiation of adult stem cells remain to be fully elucidated. In the present study, the histone H3 modifications during the transdifferentiation of rat Thy‑1(+) Lin(‑) bone marrow cells into hepatocytes in vitro were examined, which involved performing hepatocyte growth factor-mediated transdifferentiation of bone marrow Thy-1(+) Lin(‑) cells into hepatic lineage cells. Subsequently, the hepatocyte-specific markers, cytokeratin‑18 (CK‑18), albumin (ALB) and α‑fetoprotein (AFP) were examined by immunofluorescence staining or reverse transcription-quantitative polymerase chain reaction (RT-qPCR).

[Effects of abdominal compartment syndrome on circulation and respiratory function in rabbits].

Objective: To establish a rabbit model of abdominal compartment syndrome (ACS) and evaluate the impact of ACS on cardiovascular and respiratory functions and blood electrolyte levels in rabbits.

Methods: Twenty-four New Zealand rabbits were randomly allocated into 4 equal groups, namely the normal control group, ACS(5>\) group [intra-abdominal pressure (IAP)=5 mmHg], ACS(10) group (IAP=10 mmHg) and ACS(20) group (IAP=20 mmHg). ACS model was established by intra- abdominal bleeding (IAB) with intra-abdominal hypertension (IAH).

[Effects of somatostatin in a rabbit model of abdominal compartment syndrome induced by prolonged intra-abdominal hypertension].

Objective: To establish a rabbit model of abdominal compartment syndrome (ACS) induced by prolonged intra-abdominal hypertension (IAH) and evaluate the therapeutic effect of somatostatin on ACS.

Methods: Twelve New Zealand rabbits were randomized equally into normal saline (NS) group and somatostatin group. ACS model was established by intra-abdominal bleeding (IAB) and intra-abdominal infusion with nitrogen gas to achieve an intra-abdominal pressure of 15 mmHg.

Dieulafoy's lesion with intra-abdominal haemorrhage: a novel association.

Dieulafoy's lesion is an uncommon but important cause of gastrointestinal bleeding, especially with respect to the upper gastrointestinal tract wherein massive, life-threatening haemorrhage occurs from a calibre-persistent submucosal artery. This report describes a case of a 60-year-old man with gastric Dieulafoy's lesion presenting with exogastric haemorrhage, which was diagnosed following a pathological examination.

[Laparoscopic detection and treatment of closed abdominal trauma in 39 cases].

Objective: To study the clinical value of laparoscopy in the detection and treatment of closed abdominal trauma.

Methods: A retrospective analysis was conducted in 39 patients with closed abdominal trauma detected and treated laparoscopically.

Results: All the injuries were successfully diagnosed with laparoscopy, and surgical treatment was performed under laparoscope or with the assistance of laparoscopy in 35 cases, with the other 4 cases requiring conventional laparotomy.

Clinical significance of positron emission tomography for lymphoma patients.

Objective: To explore the characteristics and clinical significance of positron emission tomography (PET) examination for malignant lymphoma.

Methods: PET examination was performed in 20 patients with malignant lymphoma, among whom 16 also received examination by computed tomography (CT) or magnetic resonance imaging (MRI).

Results: PET was found to have better diagnostic efficiency than CT or MRI examination and the former provided referential information in all the cases for estimation of the clinical stage, while CT and MRI were helpful in only 66.